LOCUS       sequence-272529-       12358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PguCas13b in mammalian cells, localized in the cytoplasm. 
            For cloning of guide RNAs compatible with PguCas13b. Contains a 3' 
            direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12358)
  AUTHORS   Wessels HH, Mendez-Mancilla A, Guo X, Legut M, Daniloski Z, Sanjana 
            NE
  TITLE     Massively parallel Cas13 screens reveal principles for guide RNA 
            design.
  JOURNAL   Nat Biotechnol. 2020 Jun;38(6):722-727. doi: 
            10.1038/s41587-020-0456-9. Epub 2020 Mar 16.
  PUBMED    32518401
REFERENCE   2  (bases 1 to 12358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0456-9"; journalName: "Nat Biotechnol"; date: 
            "2020-06"; volume: "38"; issue: "6"; pages: "722-727"
COMMENT     Sequence Label: pLentiRNACRISPR_002 -
            hU6-DR_BsmBI-EFS-PguCas1...
FEATURES             Location/Qualifiers
     source          1..12358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2371..2619
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2371..2391
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2542..2561
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     CDS             complement(2641..2652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2732..2943
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6499..6525
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             6544..6600
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6601..7197
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7094..7114
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7213..7801
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7266..7286)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7684..7695)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7873..8106
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8132..8149)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8138..8362
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8408..8836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8495..8514)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8705..8726
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8845..8865)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8850..9179
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9030..9165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9092..9111
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9227..9274
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9293..9667
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9797..9918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9834..9853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9888..9907
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9967..9983)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9967..9983)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9980..10002)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9991..10007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10015..10021,10022..10039,10040..10045))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10060..10081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10198..10215)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10369..10957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10449..10468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11128..11919,11920..11988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11751..11770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11989..12093)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12168..12187)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat tccttcatat
     2401 ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca
     2461 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
     2521 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
     2581 ttcttggctt tatatatctt gtggaaagga cgaaacaccg agacggacgt ctctgttgga
     2641 tctaccctct atttgaaggg tacacacaac ttttttgaat tcgctagcta ggtcttgaaa
     2701 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2761 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
     2821 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2881 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2941 caggaccggt tctagagcgc tgccaccatg acagagcaga gcgagaggcc ctacaacggc
     3001 acctactaca ccctggaaga taagcacttc tgggccgcct ttctgaacct ggccagacac
     3061 aacgcctaca tcaccctgac acacatcgac cggcagctgg cctacagcaa ggccgacatc
     3121 accaacgacc aggacgtgct gagcttcaag gccctgtgga agaacttcga caacgacctg
     3181 gaaagaaaga gccggctgcg gagcctgatc ctgaagcact tcagctttct ggaaggcgcc
     3241 gcttacggca agaagctgtt cgagtctaag agcagcggca acaagagcag caagaacaaa
     3301 gagctgacca agaaagagaa agaggaactg caggccaacg ctctgagcct ggacaacctg
     3361 aagtctatcc tgttcgactt cctgcagaag ctgaaggact tccggaacta ctacagccac
     3421 taccggcaca gcggaagcag cgagctgcct ctgttcgacg gcaacatgct gcagcggctg
     3481 tacaacgtgt tcgacgtgtc cgtgcagcgc gtgaagatcg accacgagca caacgatgag
     3541 gtggaccctc actaccactt caaccacctc gtgcggaagg gcaagaagga cagatacggc
     3601 cacaacgaca accccagctt caagcaccac ttcgtggacg gcgaaggcat ggttacagaa
     3661 gccggcctgc tgttcttcgt gtccctgttc ctggaaaagc gggacgccat ctggatgcag
     3721 aagaagatcc ggggcttcaa aggcggcacc gagacatacc agcagatgac caacgaggtg
     3781 ttctgccggt ccagaatcag cctgcctaag ctgaagctgg aaagcctgcg gatggacgac
     3841 tggatgctgc tggacatgct gaacgaactc gtgcggtgcc ccaagcctct gtacgacaga
     3901 ctgagagagg acgaccgggc ctgctttaga gtgcccgtgg atatcctgcc tgacgaggac
     3961 gatacagatg gcggcggaga ggaccccttc aagaataccc tcgtccggca ccaggacaga
     4021 ttcccttact tcgccctgcg gtacttcgac ctgaagaagg tgttcaccag cctgcggttc
     4081 cacatcgatc tgggcaccta ccactttgcc atctacaaga agatgatcgg cgagcagccc
     4141 gaggacagac acctgaccag aaacctgtac ggcttcggcc ggatccagga cttcgccgaa
     4201 gaacacagac ccgaggaatg gaagcggctc gtcagagatc tggactactt cgagacaggc
     4261 gacaagccct acatcagcca gacaagccca cactaccaca tcgagaaggg aaagatcggc
     4321 ctgagattca tgcccgaggg ccagcatctt tggccctctc cagaagtggg caccaccaga
     4381 accggcagat ctaagtacgc ccaggacaag agactgaccg ccgaggcctt tctgtctgtg
     4441 cacgagctga tgcctatgat gttctactac ttcctgctgc gcgagaagta cagcgaggaa
     4501 gtgtctgccg agagagtgca gggcagaatc aagcgcgtga tcgaggatgt gtacgccgtg
     4561 tacgatgcct ttgccaggga cgagatcaac acccgggatg agctggatgc ctgcctggcc
     4621 gataagggca tcagaagagg ccatctgcct cgccagatga tcgccatcct gagccaagag
     4681 cacaaggaca tggaagagaa gattcggaag aaactgcaag agatgatggc cgacaccgac
     4741 caccggctgg atatgctgga tagacagacc gaccggaaga tcagaatcgg ccggaagaat
     4801 gccggactgc ctaagagcgg agtgatcgcc gattggctcg tgcgggacat gatgagattt
     4861 cagcccgtgg ccaaggacgc cagcggaaag cctctgaaca actccaaggc caacagcacc
     4921 gagtaccgga tgctgcagag agccctggct ctgtttggag gcgagaaaga gaggctgacc
     4981 ccatacttcc ggcagatgaa tctgaccggc ggaaacaacc ctcatccttt tctgcacgaa
     5041 actcgctggg agagccacac caacatcctg tccttttacc ggtcctacct gagagcccgc
     5101 aaggccttcc tcgaaaggat cggcagatcc gacagagtgg aaaaccggcc atttctgctg
     5161 ctgaaagagc ccaagaccga cagacagaca ctggtggctg gatggaaggg cgagttccat
     5221 ctgccaagag gcatcttcac agaggccgtg cgggattgcc tgatcgagat gggacacgat
     5281 gaagtggcca gctacaaaga agtgggattc atggccaagg ccgtgccact gtactttgag
     5341 agagcctgcg aggacagggt gcagcccttt tacgacagcc ccttcaacgt gggcaacagc
     5401 ctgaagccta agaagggcag attcctgagc aaagaggaac gggccgaaga gtgggagcgg
     5461 ggcaaagaga gattccggga tctcgaagcc tggtcctata gcgccgccag aagaatcgag
     5521 gacgcctttg ccggcatcga gtatgctagc cccggaaaca agaagaaaat cgagcagctc
     5581 ctgcgggacc tgagcctgtg ggaagccttt gagagcaagc tgaaagtgcg ggccgaccgg
     5641 atcaatctgg ccaagctgaa gaaagaaatc ctggaagccc aagaacaccc ctaccacgac
     5701 ttcaagagct ggcagaagtt cgagcgcgag ctgcggctgg tcaagaacca ggatatcatc
     5761 acctggatga tgtgccgcga cctgatggaa gaaaacaagg tggaaggcct ggacaccggc
     5821 acactgtacc tgaaggacat cagacccaac gtgcaagagc agggcagcct gaacgtgctg
     5881 aacagagtga agcccatgag actgcccgtg gtggtgtaca gagccgatag cagaggccac
     5941 gtgcacaaag aagaagcccc tctggccacc gtgtacatcg aggaaagaga caccaagctg
     6001 ctgaagcagg gcaacttcaa gtccttcgtg aaggacaggc ggctgaacgg cctgttctcc
     6061 ttcgttgata ctggcggact ggccatggaa cagtacccca tctccaagct gagagtggaa
     6121 tacgagctgg ccaagtacca gaccgccaga gtgtgtgtgt tcgagctgac cctgagactg
     6181 gaagagtccc tgctgacccg gtatcctcat ctgcccgacg agagcttcag agagatgctg
     6241 gaatcttgga gcgaccctct gctggccaaa tggcctgaac tgcacggaaa agtgcggctg
     6301 ctgatcgccg tgcggaatgc ctttagccac aatcagtacc ctatgtacga cgaggccgtg
     6361 ttcagctcca tccggaagta cgatcctagc agccccgacg ccatcgaaga gagaatgggc
     6421 ctgaatatcg cccaccgcct gtctgaggaa gtgaagcagg ccaaagaaac cgtggaacgg
     6481 atcattcagg cccttcaact gcctccactt gaaagactga cactgggtag ttccggatcc
     6541 ggcgcaacaa acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg
     6601 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6661 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6721 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6781 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6841 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6901 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6961 gcgtggttcc tggccaccgt cggagtctcg cccgaccacc agggcaaggg tctgggcagc
     7021 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7081 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7141 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaacg
     7201 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7261 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7321 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7381 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7441 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7501 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7561 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     7621 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7681 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7741 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7801 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7861 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7921 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7981 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8041 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8101 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     8161 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     8221 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     8281 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     8341 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     8401 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8461 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8521 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8581 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     8641 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8701 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     8761 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     8821 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     8881 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     8941 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     9001 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     9061 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     9121 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     9181 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     9241 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     9301 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     9361 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     9421 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     9481 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     9541 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     9601 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     9661 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     9721 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     9781 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9841 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9901 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     9961 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10021 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10081 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10141 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10201 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10261 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10321 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10381 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10441 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10501 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10561 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10621 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10681 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10741 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10801 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10861 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10921 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10981 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11041 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11101 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11161 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11221 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11281 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11341 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11401 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11461 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11521 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11581 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11641 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11701 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11761 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11821 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11881 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11941 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12001 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12061 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    12121 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
    12181 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
    12241 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
    12301 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
