LOCUS       sequence-272644-       10349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  R615A UPF1 inserted with GFP tagged on the N-terminus used to stably
            integrate into cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Fischer JW, Busa VF, Shao Y, Leung AKL
  TITLE     Structure-Mediated RNA Decay by UPF1 and G3BP1.
  JOURNAL   Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 
            10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3.
  PUBMED    32017897
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.021"; journalName: "Mol Cell"; date: 
            "2020-04-2- 2"; volume: "78"; issue: "1"; pages: "70-84.e6"
COMMENT     Sequence Label: pci-EGFP-UPF1-R615A
FEATURES             Location/Qualifiers
     source          1..10349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     182..203
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3684..3805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3695..3714)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3749..3768
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3991..4446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4563..4920
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4563..4754
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4586..4606)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4771..4906
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4971..5765
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5025..5044)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5635..5654
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5829..5877
                     /note="synthetic polyadenylation signal"
     promoter        complement(5953..5971)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5985..6001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5985..6001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5998..6020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6009..6025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6033..6039,6040..6057,6058..6063))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6078..6099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6216..6233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6387..6975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6467..6486)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7146..7937,7938..8006))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7769..7788
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8007..8111)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8138..8593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     8719..8741
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8733..8750
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8734..8750
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        8911..9290
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9291..9494
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9445..9465
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9661..9793
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     9776..9795
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        9838..9856
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      9872..9881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9878..9880,9881..9883,9884..10349,1..245)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9878..9880,9881..9883,9884..10349,1..245)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9923..9944)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10184..10203)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
       61 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      121 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      181 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      241 acaagcttag cgtggaggcg tacgggccca gctcgcagac tctcactttc ctggacacgg
      301 aggaggccga gctgcttggc gccgacacac agggctccga gttcgagttc accgacttta
      361 ctcttcctag ccagacgcag acgccccccg gcggccccgg cggcccgggc ggtggcggcg
      421 cgggaggccc gggcggcgcg ggcgcgggcg ctgcggcggg acagctcgac gcgcaggttg
      481 ggcccgaagg catcctgcag aacggggctg tggacgacag tgtagccaag accagccagt
      541 tgttggctga gttgaacttc gaggaagatg aagaagacac ctattacacg aaggacctcc
      601 ccatacacgc ctgcagttac tgtggaatac acgatcctgc ctgcgtggtt tactgtaata
      661 ccagcaagaa gtggttctgc aacggacgtg gaaatacttc tggcagccac attgtaaatc
      721 accttgtgag ggcaaaatgc aaagaggtga ccctgcacaa ggacgggccc ctgggggaga
      781 cagtcctgga gtgctacaac tgcggctgtc gcaacgtctt cctcctcggc ttcatcccgg
      841 ccaaagctga ctcagtggtg gtgctgctgt gcaggcagcc ctgtgccagc cagagcagcc
      901 tcaaggacat caactgggac agctcgcagt ggcagccgct gatccaggac cgctgcttcc
      961 tgtcctggct ggtcaagatc ccctccgagc aggagcagct gcgggcacgc cagatcacgg
     1021 cacagcagat caacaagctg gaggagctgt ggaaggaaaa cccttctgcc acgctggagg
     1081 acctggagaa gccgggggtg gacgaggagc cgcagcatgt cctcctgcgg tacgaggacg
     1141 cctaccagta ccagaacata ttcgggcccc tggtcaagct ggaggccgac tacgacaaga
     1201 agctgaagga gtcccagact caagataaca tcactgtcag gtgggacctg ggccttaaca
     1261 agaagagaat cgcctacttc actttgccca agactgactc tgacatgcgg ctcatgcagg
     1321 gggatgagat atgcctgcgg tacaaagggg accttgcgcc cctgtggaaa gggatcggcc
     1381 acgtcatcaa ggtccctgat aattatggcg atgagatcgc cattgagctg cggagcagcg
     1441 tgggtgcacc tgtggaggtg actcacaact tccaggtgga ttttgtgtgg aagtcgacct
     1501 cctttgacag gatgcagagc gcattgaaaa cgtttgccgt ggatgagacc tcggtgtctg
     1561 gctacatcta ccacaagctg ttgggccacg aggtggagga cgtaatcatc aagtgccagc
     1621 tgcccaagcg cttcacggcg cagggcctcc ccgacctcaa ccactcccag gtttatgccg
     1681 tgaagactgt gctgcaaaga ccactgagcc tgatccaggg cccgccaggc acggggaaga
     1741 cggtgacgtc ggccaccatc gtctaccacc tggcccggca aggcaacggg ccggtgctgg
     1801 tgtgtgctcc gagcaacatc gccgtggacc agctaacgga gaagatccac cagacggggc
     1861 taaaggtcgt gcgcctctgc gccaagagcc gtgaggccat cgactccccg gtgtcttttc
     1921 tggccctgca caaccagatc aggaacatgg acagcatgcc tgagctgcag aagctgcagc
     1981 agctgaaaga cgagactggg gagctgtcgt ctgccgacga gaagcggtac cgggccttga
     2041 agcgcaccgc agaggcagag ctgctgatga acgcagatgt catctgctgc acatgtgtgg
     2101 gcgccggtga cccgaggctg gccaagatgc agttccgctc cattttaatc gacgaaagca
     2161 cccaggccac cgagccggag tgcatggttc ccgtggtcct cggggccaag cagctgatcc
     2221 ttgtaggcga ccactgccag ctgggcccag tggtgatgtg caagaaggcg gccaaggccg
     2281 ggctgtcaca gtcgctcttc gagcgcctgg tggtgctggg catccggccc atccgcctgc
     2341 aggtccagta ccggatgcac cctgcactca gcgccttccc atccaacatc ttctacgagg
     2401 gctccctcca gaatggtgtc actgcagcgg atcgtgtgaa gaagggattt gacttccagt
     2461 ggccccaacc cgataaaccg atgttcttct acgtgaccca gggccaagag gagattgcca
     2521 gctcgggcac ctcctacctg aacaggaccg aggctgcgaa cgtggagaag atcaccacga
     2581 agttgctgaa ggcaggcgcc aagccggacc agattggcat catcacgccc tacgagggcc
     2641 agcgctccta cctggtgcag tacatgcagt tcagcggctc cctgcacacc aagctctacc
     2701 aggaggtgga gatcgccagt gtggacgcct ttcagggacg cgagaaggac ttcatcatcc
     2761 tgtcctgtgt gcgggccaac gagcaccaag gcattggctt tttaaatgac cccaggcgtc
     2821 tgaacgtggc cctgaccaga gcaaggtatg gcgtcatcat tgtgggcaac ccgaaggcac
     2881 tatcaaagca gccgctctgg aaccacctgc tgaactacta taaggagcag aaggtgctgg
     2941 tggaggggcc gctcaacaac ctgcgtgaga gcctcatgca gttcagcaag ccacggaagc
     3001 tggtcaacac tatcaacccg ggagcccgct tcatgaccac agccatgtat gatgcccggg
     3061 aggccatcat cccaggctcc gtctatgatc ggagcagcca gggccggcct tccagcatgt
     3121 acttccagac ccatgaccag attggcatga tcagtgccgg ccctagccac gtggctgcca
     3181 tgaacattcc catccccttc aacctggtca tgccacccat gccaccgcct ggctattttg
     3241 gacaagccaa cgggcctgct gcagggcgag gcaccccgaa aggcaagact ggtcgtgggg
     3301 gacgccagaa gaaccgcttt gggcttcctg gacccagcca gactaacctc cccaacagcc
     3361 aagccagcca ggatgtggcg tcacagccct tctctcaggg cgccctgacg cagggctaca
     3421 tctccatgag ccagccttcc cagatgagcc agcccggcct ctcccagccg gagctgtccc
     3481 aggacagtta ccttggtgac gagtttaaat cacaaatcga cgtggcgctc tcacaggact
     3541 ccacgtacca gggagagcgg gcttaccagc atggcggggt gacggggctg tcccagtatt
     3601 aaaaggtggc ggcggaagag ctaaagagtc gacccgggcg gccgcttccc tttagtgagg
     3661 gttaatgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3721 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3781 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     3841 tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     3901 ccgataagga tcgatccggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     3961 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     4021 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4081 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4141 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4201 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4261 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4321 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4381 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4441 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     4501 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     4561 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4621 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4681 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4741 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4801 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     4861 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4921 aagcttgatt cttctgacac aacagtctcg aacttaaggc tagagccacc atgattgaac
     4981 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5041 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5101 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5161 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5221 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5281 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5341 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5401 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5461 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     5521 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5581 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5641 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5701 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5761 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5821 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     5881 cgatagcgat aaggatcccc taggcttttg caaaaagctc ctcgaggaac tgaaaaacca
     5941 gaaagttaat tccctatagt gagtcgtatt aaattcgtaa tcatgtcata gctgtttcct
     6001 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6061 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6121 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6181 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6241 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6301 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6361 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6421 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6481 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6541 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6601 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6661 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6721 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6781 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6841 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6901 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6961 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7021 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7081 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7141 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7201 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7261 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7321 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7381 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7441 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7501 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7561 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7621 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7681 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7741 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7801 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7861 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7921 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7981 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8041 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8101 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta
     8161 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8221 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8281 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8341 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8401 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8461 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8521 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8581 acgcttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     8641 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     8701 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga
     8761 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
     8821 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
     8881 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
     8941 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     9001 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9061 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9121 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
     9181 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
     9241 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     9301 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     9361 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     9421 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     9481 atataagcag agctcgttta gtgaaccgtc agaattgatc taccatgagc tttattgcgg
     9541 tagtttatca cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact
     9601 taagctgcag tgactctctt aaggtagcct tgcagaagtt ggtcgtgagg cactgggcag
     9661 gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga
     9721 cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc
     9781 tttctctcca caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa
     9841 tacgactcac tataggctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct
     9901 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     9961 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
    10021 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
    10081 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
    10141 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
    10201 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
    10261 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
    10321 ccacaacgtc tatatcatgg ccgacaagc
//
