LOCUS       sequence-272720-       16689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Myc and Klf4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16689)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 16689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S EM2K
FEATURES             Location/Qualifiers
     source          1..16689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..1175
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          244..1166
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    2385..2958
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2408..2960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    3977..4111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4644..5806
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4875..5797
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             5938..7257
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     misc_feature    7324..7897
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7347..7899
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     polyA_signal    9384..9518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      10251..12044
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(12346..14271)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGGGGXGGGGGGGGGGGAGGAGGAGAGGGGGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGXGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGGGGAGGGGGGGGGGAG
                     GGXGGGGGGGGGGGGGGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(14710..14728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        14796..14900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14901..14969,14970..15761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15119..15138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15932..16520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16421..16440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagcttcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
       61 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      121 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      181 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      241 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      301 gtgccttgaa ttacttccac ctggctccag tacgtgattc ttgatcccga gctggagcca
      361 ggggcgggcc ttgcgcttta ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      421 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      481 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      541 atagtcttgt aaatgcgggc caggatctgc acactggtat ttcggttttt ggggccgcgg
      601 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      661 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      721 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      781 cgtgagcgga aagatggccg cttcccggcc ctgctccagg gggctcaaaa tggaggacgc
      841 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaggggcc tttccgtcct
      901 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
      961 tctggagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1021 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1081 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1141 ttcaaagttt ttttcttcca tttcaggtgt cgtgaacacg tggtcgcggc caattctgca
     1201 gtcgagctca agcttcgaac tagtgattat ggcgggacac ctggcttcgg atttcgcctt
     1261 ctcgccccct ccaggtggtg gaggtgatgg gccagggggg ccggagccgg gctgggttga
     1321 tcctcggacc tggctaagct tccaaggccc tcctggaggg ccaggaatcg ggccgggggt
     1381 tgggccaggc tctgaggtgt gggggattcc cccatgcccc ccgccgtatg agttctgtgg
     1441 ggggatggcg tactgtgggc cccaggttgg agtggggcta gtgccccaag gcggcttgga
     1501 gacctctcag cctgagggtg aagcaggagt cggggtggag agcaactccg atggggcctc
     1561 cccggagccc tgcaccgtca cccctggtgc cgtgaagctg gagaaggaga agctggagca
     1621 aaacccggag gagtcccagg acatcaaagc tctgcagaaa gaactcgagc aatttgccaa
     1681 gctcctgaag cagaagagga tcaccctggg atatacacag gccgatgtgg ggctcaccct
     1741 gggggttcta tttgggaagg tattcagcca aacgaccatc tgccgctttg aggctctgca
     1801 gcttagcttc aagaacatgt gtaagctgcg gcccttgctg cagaagtggg tggaggaagc
     1861 tgacaacaat gaaaatcttc aggagatatg caaagcagaa accctcgtgc aggcccgaaa
     1921 gagaaagcga accagtatcg agaaccgagt gagaggcaac ctggagaatt tgttcctgca
     1981 gtgcccgaaa cccacactgc agcagatcag ccacatcgcc cagcagcttg ggctcgagaa
     2041 ggatgtggtc cgagtgtggt tctgtaaccg gcgccagaag ggcaagcgat caagcagcga
     2101 ctatgcacaa cgagaggatt ttgaggctgc tgggtctcct ttctcagggg gaccagtgtc
     2161 ctttcctctg gccccagggc cccattttgg taccccaggc tatgggagcc ctcacttcac
     2221 tgcactgtac tcctcggtcc ctttccctga gggggaagcc tttccccctg tctccgtcac
     2281 cactctgggc tctcccatgc attcaaactg aggtgcctgc cctctagttt acgcgtgggg
     2341 gcggccggcc gctttctcga ggccatcgat gtttaaacga tccgcccctc tccctccccc
     2401 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     2461 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     2521 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     2581 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     2641 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     2701 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     2761 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     2821 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt
     2881 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     2941 tgaaaaacac gatgataata tggccacacc gcgaattaat tcgattccgc atgtacaaca
     3001 tgatggagac ggagctgaag ccgccgggcc cgcagcaaac ttcggggggc ggcggcggca
     3061 actccaccgc ggcggcggcc ggcggcaacc agaaaaacag cccggaccgc gtcaagcggc
     3121 ccatgaatgc cttcatggtg tggtcccgcg ggcagcggcg caagatggcc caggagaacc
     3181 ccaagatgca caactcggag atcagcaagc gcctgggcgc cgagtggaaa cttttgtcgg
     3241 agacggagaa gcggccgttc atcgacgagg ctaagcggct gcgagcgctg cacatgaagg
     3301 agcacccgga ttataaatac cggccccggc ggaaaaccaa gacgctcatg aagaaggata
     3361 agtacacgct gcccggcggg ctgctggccc ccggcggcaa tagcatggcg agcggggtcg
     3421 gggtgggcgc cggcctgggc gcgggcgtga accagcgcat ggacagttac gcgcacatga
     3481 acggctggag caacggcagc tacagcatga tgcaggacca gctgggctac ccgcagcacc
     3541 cgggcctcaa tgcgcacggc gcagcgcaga tgcagcccat gcaccgctac gacgtgagcg
     3601 ccctgcagta caactccatg accagctcgc agacctacat gaacggctcg cccacctaca
     3661 gcatgtccta ctcgcagcag ggcacccctg gcatggctct tggctccatg ggttcggtgg
     3721 tcaagtccga ggccagctcc agcccccctg tggttacctc ttcctcccac tccagggcgc
     3781 cctgccaggc cggggacctc cgggacatga tcagcatgta tctccccggc gccgaggtgc
     3841 cggaacccgc cgcccccagc agacttcaca tgtcccagca ctaccagagc ggcccggtgc
     3901 ccggcacggc cattaacggc acactgcccc tctcacacat gtgagggccg gaaatcacta
     3961 gctagttcga aggatcgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4021 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4081 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     4141 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     4201 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     4261 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4321 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgacc
     4381 aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca
     4441 ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat
     4501 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
     4561 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca
     4621 ttgattattg actagcttcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     4681 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     4741 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     4801 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     4861 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     4921 atggcccttg cgtgccttga attacttcca cctggctcca gtacgtgatt cttgatcccg
     4981 agctggagcc aggggcgggc cttgcgcttt aggagcccct tcgcctcgtg cttgagttga
     5041 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     5101 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     5161 tttctggcaa gatagtcttg taaatgcggg ccaggatctg cacactggta tttcggtttt
     5221 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     5281 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     5341 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     5401 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctccag ggggctcaaa
     5461 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaggggc
     5521 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     5581 cctcgattag ttctggagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     5641 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     5701 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     5761 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaacac gtggtcgcgg
     5821 ccaattctgc agtcgagctc aagcttcgaa ctagggccgc gactctagaa gctgggtacc
     5881 agctgctagc gctaccggac tcagatctcg agctcaagct tcgaattcgc gatcgccatg
     5941 cccctcaacg ttagcttcac caacaggaac tatgacctcg actacgactc ggtgcagccg
     6001 tatttctact gcgacgagga ggagaacttc taccagcagc agcagcagag cgagctgcag
     6061 cccccggcgc ccagcgagga tatctggaag aaattcgagc tgctgcccac cccgcccctg
     6121 tcccctagcc gccgctccgg gctctgctcg ccctcctacg ttgcggtcac acccttctcc
     6181 cttcggggag acaacgacgg cggtggcggg agcttctcca cggccgacca gctggagatg
     6241 gtgaccgagc tgctgggagg agacatggtg aaccagagtt tcatctgcga cccggacgac
     6301 gagaccttca tcaaaaacat catcatccag gactgtatgt ggagcggctt ctcggccgcc
     6361 gccaagctcg tctcagagaa gctggcctcc taccaggctg cgcgcaaaga cagcggcagc
     6421 ccgaaccccg cccgcggcca cagcgtctgc tccacctcca gcttgtacct gcaggatctg
     6481 agcgccgccg cctcagagtg catcgacccc tcggtggtct tcccctaccc tctcaacgac
     6541 agcagctcgc ccaagtcctg cgcctcgcaa gactccagcg ccttctctcc gtcctcggat
     6601 tctctgctct cctcgacgga gtcctccccg cagggcagcc ccgagcccct ggtgctccat
     6661 gaggagacac cgcccaccac cagcagcgac tctgaggagg aacaagaaga tgaggaagaa
     6721 atcgatgttg tttctgtgga aaagaggcag gctcctggca aaaggtcaga gtctggatca
     6781 ccttctgctg gaggccacag caaacctcct cacagcccac tggtcctcaa gaggtgccac
     6841 gtctccacac atcagcacaa ctacgcagcg cctccctcca ctcggaagga ctatcctgct
     6901 gccaagaggg tcaagttgga cagtgtcaga gtcctgagac agatcagcaa caaccgaaaa
     6961 tgcaccagcc ccaggtcctc ggacaccgag gagaatgtca agaggcgaac acacaacgtc
     7021 ttggagcgcc agaggaggaa cgagctaaaa cggagctttt ttgccctgcg tgaccagatc
     7081 ccggagttgg aaaacaatga aaaggccccc aaggtagtta tccttaaaaa agccacagca
     7141 tacatcctgt ccgtccaagc agaggagcaa aagctcattt ctgaagagga cttgttgcgg
     7201 aaacgacgag aacagttgaa acacaaactt gaacagctac ggaactcttg tgcgtaagtt
     7261 taaacatcga tggcctcgag aaagcggccg ctttctcgag gccatcgatg tttaaacgat
     7321 ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     7381 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     7441 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     7501 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     7561 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     7621 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     7681 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     7741 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     7801 gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     7861 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaccg cgatcgcgaa
     7921 ttcgcgatcg ccatggctgt cagcgacgcg ctgctcccat ctttctccac gttcgcgtct
     7981 ggcccggcgg gaagggagaa gacactgcgt caagcaggtg ccccgaataa ccgctggcgg
     8041 gaggagctct cccacatgaa gcgacttccc ccagtgcttc ccggccgccc ctatgacctg
     8101 gcggcggcga ccgtggccac agacctggag agcggcggag ccggtgcggc ttgcggcggt
     8161 agcaacctgg cgcccctacc tcggagagag accgaggagt tcaacgatct cctggacctg
     8221 gactttattc tctccaattc gctgacccat cctccggagt cagtggccgc caccgtgtcc
     8281 tcgtcagcgt cagcctcctc ttcgtcgtcg ccgtcgagca gcggccctgc cagcgcgccc
     8341 tccacctgca gcttcaccta tccgatccgg gccgggaacg acccgggcgt ggcgccgggc
     8401 ggcacgggcg gaggcctcct ctatggcagg gagtccgctc cccctccgac ggctcccttc
     8461 aacctggcgg acatcaacga cgtgagcccc tcgggcggct tcgtggccga gctcctgcgg
     8521 ccagaattgg acccggtgta cattccgccg cagcagccgc agccgccagg tggcgggctg
     8581 atgggcaagt tcgtgctgaa ggcgtcgctg agcgcccctg gcagcgagta cggcagcccg
     8641 tcggtcatca gcgtcagcaa aggcagccct gacggcagcc acccggtggt ggtggcgccc
     8701 tacaacggcg ggccgccgcg cacgtgcccc aagatcacgc aggaggcggt ctcttcgtgc
     8761 acccacttgg gcgctggacc ccctctcagc aatggccacc ggccggctgc acacgacttc
     8821 cccctggggc ggcagctccc cagcaggact accccgaccc tgggtcttga ggaagtgctg
     8881 agcagcaggg actgtcaccc tgccctgccg cttcctcccg gcttccatcc ccacccgggg
     8941 cccaattacc catccttcct gcccgatcag atgcagccgc aagtcccgcc gctccattac
     9001 caagagctca tgccacccgg ttcctgcatg ccagaggagc ccaagccaaa gaggggaaga
     9061 cgatcgtggc cccggaaaag gaccgccacc cacacttgtg attacgcggg ctgcggcaaa
     9121 acctacacaa agagttccca tctcaaggca cacctgcgaa cccacacagg tgagaaacct
     9181 taccactgtg actgggacgg ctgtggatgg aaattcgccc gctcagatga actgaccagg
     9241 cactaccgta aacacacggg gcaccgcccg ttccagtgcc aaaaatgcga ccgagcattt
     9301 tccaggtcgg accacctcgc cttacacatg aagaggcatt tttaaatccc agacagtgga
     9361 tatgacccgt ttctagttcg aaggatccag acatgataag atacattgat gagtttggac
     9421 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     9481 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     9541 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     9601 aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa
     9661 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     9721 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag
     9781 gtcgatcgac tctagctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga
     9841 catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact
     9901 tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc
     9961 tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc
    10021 atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact
    10081 cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga
    10141 ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc
    10201 ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc
    10261 atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt
    10321 tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc
    10381 gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag
    10441 ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac
    10501 tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg
    10561 agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc
    10621 agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc
    10681 tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa
    10741 caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat
    10801 ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg
    10861 tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga
    10921 aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt
    10981 aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg
    11041 aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta
    11101 aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact
    11161 tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca
    11221 agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc
    11281 caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg
    11341 ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
    11401 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
    11461 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
    11521 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
    11581 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
    11641 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
    11701 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
    11761 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
    11821 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
    11881 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata
    11941 tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc
    12001 acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt gctcccattc
    12061 ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg tctgattgct caccaggtaa
    12121 atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt gacgtgacaa
    12181 catgggtatg cccaattgcc ccatgttggg aggacgaaaa tggtgacaag acagatggcc
    12241 agaaatacac caacagcacg catgatgtct actggggatt tattctttag tgcgggggaa
    12301 tacacggctt ttaatacgat tgagggcgtc tcctaacaag ttacatcact cctgcccttc
    12361 ctcaccctca tctccatcac ctccttcatc tccgtcatct ccgtcatcac cctccgcggc
    12421 agccccttcc accataggtg gaaaccaggg aggcaaatct actccatcgt caaagctgca
    12481 cacagtcacc ctgatattgc aggtaggagc gggctttgtc ataacaaggt ccttaatcgc
    12541 atccttcaaa acctcagcaa atatatgagt ttgtaaaaag accatgaaat aacagacaat
    12601 ggactccctt agcgggccag gttgtgggcc gggtccaggg gccattccaa aggggagacg
    12661 actcaatggt gtaagacgac attgtggaat agcaagggca gttcctcgcc ttaggttgta
    12721 aagggaggtc ttactacctc catatacgaa cacaccggcg acccaagttc cttcgtcggt
    12781 agtcctttct acgtgactcc tagccaggag agctcttaaa ccttctgcaa tgttctcaaa
    12841 tttcgggttg gaacctcctt gaccacgatg ctttccaaac caccctcctt ttttgcgcct
    12901 gcctccatca ccctgacccc ggggtccagt gcttgggcct tctcctgggt catctgcggg
    12961 gccctgctct atcgctcccg ggggcacgtc aggctcacca tctgggccac cttcttggtg
    13021 gtattcaaaa taatcggctt cccctacagg gtggaaaaat ggccttctac ctggaggggg
    13081 cctgcgcggt ggagacccgg atgatgatga ctgactactg ggactcctgg gcctcttttc
    13141 tccacgtcca cgacctctcc ccctggctct ttcacgactt ccccccctgg ctctttcacg
    13201 tcctctaccc cggcggcctc cactacctcc tcgaccccgg cctccactac ctcctcgacc
    13261 ccggcctcca ctgcctcctc gaccccggcc tccacctcct gctcctgccc ctcctgctcc
    13321 tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
    13381 tcctcctccc cctcctcccc ctcctcctcc tccccctcct scccctcctc ctgctcctcc
    13441 ccctcctccc cctcctcctc ctcctgcccc tcctccccct cctgctcctg cccctcctgc
    13501 ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc
    13561 tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
    13621 ccctcctgct cctgcccctc ctsccccccc tgcccctcct gctcctgccc ctcctcctgc
    13681 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc
    13741 tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc
    13801 tgcccctcct gcccctcctc ctgctcctcc ccctcctcct gctcctgccc ctcctgcccc
    13861 tcctgcccct cctcctcctc ctccccctcc tcctcctcct scccctcctc cccctcctgc
    13921 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc
    13981 ccctcccgct cctgctcctg ctcctgttcc accgtgggtc cctttgcagc caatgcaact
    14041 tggacgtttt tggggtctcc ggacaccatc tctatgtctt ggccctgatc ctgagccgcc
    14101 cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt
    14161 atcaccccct cttctttgag gtccactgcc gccggagcct tctggtccag atgtgtctcc
    14221 cttctctcct aggccatttc caggtcctgt acctggcccc tcgtcagaca tgattcacac
    14281 taaaagagat caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac
    14341 tcctgccccc tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat
    14401 ccaggtctga aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca
    14461 gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct
    14521 caaattcctc gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct
    14581 cttcctcttc aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg
    14641 cggcctgtga ggatcagctt atcgatgata agctgtcaaa catgagaatt aattcttgaa
    14701 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
    14761 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
    14821 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
    14881 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
    14941 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
    15001 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
    15061 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
    15121 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
    15181 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
    15241 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
    15301 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
    15361 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
    15421 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
    15481 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
    15541 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
    15601 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
    15661 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
    15721 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
    15781 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
    15841 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
    15901 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
    15961 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
    16021 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
    16081 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
    16141 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
    16201 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
    16261 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
    16321 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
    16381 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
    16441 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
    16501 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
    16561 gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
    16621 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
    16681 ctcgcgtcg
//
