LOCUS       sequence-27311-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: FLAG-Ago2
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..104
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    117..141
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagaat taaccatgga ctacaaagac catgacggtg
       61 attataaaga tcatgacatc gattacaagg atgacgatga caagcttgcg gccatcacaa
      121 gtttgtacaa aaaagcaggc tctttaaagg aaccaattca gtcgactgga tccatgcacc
      181 cattccagtg gtgtaacgca cttgcacctc ctgcgccgcc gccccccatc caaggatatg
      241 ccttcaagcc tccacctaga cccgactttg ggacctccgg gagaacaatc aaattacagg
      301 ccaatttctt cgaaatggac atccccaaaa ttgacatcta tcattatgaa ttggatatca
      361 agccagagaa gtgcccgagg agagttaaca gggaaatcgt ggaacacatg gtccagcact
      421 ttaaaacaca gatctttggg gatcggaagc ccgtgtttga cggcaggaag aatctataca
      481 cagccatgcc ccttccgatt gggagggaca aggtggagct ggaggtcacg ctgccaggag
      541 aaggcaagga tcgcatcttc aaggtgtcca tcaagtgggt gtcctgcgtg agcttgcagg
      601 cgttacacga tgcactttca gggcggctgc cccagcgtcc cttttgagac gatccaggcc
      661 ctggacgtgg tcatgaggca cttgccatcc atgaggtaca cccccgtggg ccgctccttc
      721 ttcaccgcgt ccgaaggctg ctctaaccct cttggcgggg gccgagaagt gtggtttggc
      781 t
//
