LOCUS       sequence-273329-        8741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A single vector mammalian expression system containing a CAG 
            promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 
            LMD9:LMG18311 chimera) and its U6-driven sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Agudelo D, Carter S, Velimirovic M, Duringer A, Rivest JF, Levesque 
            S, Loehr J, Mouchiroud M, Cyr D, Waters PJ, Laplante M, Moineau S, 
            Goulet A, Doyon Y
  TITLE     Versatile and robust genome editing with Streptococcus thermophilus 
            CRISPR1-Cas9.
  JOURNAL   Genome Res. 2020 Jan;30(1):107-117. doi: 10.1101/gr.255414.119. Epub
            2020 Jan 3.
  PUBMED    31900288
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.255414.119"; journalName: "Genome Res"; date: "2020-01";
            volume: "30"; issue: "1"; pages: "107-117"
COMMENT     Sequence Label: U6_sgRNA_CAG_hSt1Cas9_LMG18311
FEATURES             Location/Qualifiers
     source          1..8741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        94..342
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     94..114
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     265..284
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        514..817
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1095..2111
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2035..2057
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2119..2138
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2204..2213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2219..2239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2264..5626
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus 
                     thermophilus Type II CRISPR/Cas system (Esvelt et al., 
                     2013)"
                     /label=St1Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="SDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVR
                     RTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEEL
                     FIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQ
                     LRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKR
                     KYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLLN
                     DLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRIDK
                     SGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADGS
                     FSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKTT
                     SSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNEDD
                     EKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERCL
                     YTGKTISIHDLINNSNQFEVDHILPLSITFDDSLANKVLVYATANQEKGQRTPYQALDS
                     MDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTRYASRVVL
                     NALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNLW
                     KKQKNTLVSYSEEQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSYQ
                     VDSKFNRKISDATIYATRQAKVGKDKKDETYVLGKIKDIYTQDGYDAFMKIYKKDKSKF
                     LMYRHDPQTFEKVIEPILENYPNKQMNEKGKEVPCNPFLKYKEEHGYIRKYSKKGNGPE
                     IKSLKYYDSKLLGNPIDITPENSKNKVVLQSLKPWRTDVYFNKNTGKYEILGLKYADLQ
                     FEKKTGTYKISQEKYNGIMKEEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRT
                     MPNVKYYVELKPYSKDKFEKNESLIEILGSADKSGRCIKGLGKSNISIYKVRTDVLGNQ
                     HIIKNEGDKPKLDF"
     CDS             5639..5659
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5763..5782)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5828..5883
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5882..5901)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6244..6260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6244..6260)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6257..6279)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6268..6284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6292..6298,6299..6316,6317..6322))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6337..6358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6417..6612
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6463..6598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6618..6752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6655..6674)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6709..6728
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6820..6837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6991..7579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7071..7090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7750..8541,8542..8610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8373..8392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8611..8715)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagctgtaca aaaaagcagg ctttaaagga accaattcag
       61 tcgactggat ccggtaccaa ggtcgggcag gaagagggcc tatttcccat gattccttca
      121 tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac
      181 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
      241 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
      301 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca cccgagacga ttaatgcgtc
      361 tcggtctttg tactctggta ccagaagcta caaagataag gcttcatgcc gaaatcaaca
      421 ccctgtcatt ttatggcagg gtgttttttt tactagttat taatagtaat caattacggg
      481 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      541 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      601 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      661 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      721 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      781 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      841 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      901 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgccrggg gggggggggg
      961 gggggggggg gsggggsggg gsgrggsggr gaggtgcggc ggcagccaat cagagcggcg
     1021 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     1081 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
     1141 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     1201 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
     1261 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
     1321 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
     1381 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     1441 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     1501 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     1561 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     1621 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     1681 tgccgggcgg ggcggggccg cctcgggccg gggrgggctc gggggagggg cgcggcggcc
     1741 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     1801 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     1861 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     1921 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     1981 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     2041 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     2101 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     2161 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tgggcgcccc
     2221 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccagcgacc tggtgctggg
     2281 cctggacatc ggcatcggca gcgtgggcgt gggcatcctg aacaaggtga ccggcgagat
     2341 catccacaag aacagtcgca tcttccctgc tgctcaggct gagaacaacc tggtgcgccg
     2401 caccaaccgc cagggtcgcc ggcttgctcg ccgcaagaag caccggcgcg tgcgcctgaa
     2461 ccgcctgttc gaggagagcg gcctgatcac cgacttcacc aagatcagca tcaacctgaa
     2521 cccctaccag ctgcgcgtga agggcctgac cgacgagctg agcaacgagg agctgttcat
     2581 cgccctgaag aacatggtga agcaccgcgg catcagctac ctggacgacg ccagcgacga
     2641 cggcaacagc agcgtgggcg actacgccca gatcgtgaag gagaacagca agcagctgga
     2701 gaccaagacc cccggccaga tccagctgga gcgctaccag acctacggcc agctgcgcgg
     2761 cgacttcacc gtggagaagg acggcaagaa gcaccgcctg atcaacgtgt tccccaccag
     2821 cgcctaccgc agcgaggccc tgcgcatcct gcagacccag caggagttca acccccagat
     2881 caccgacgag ttcatcaacc gctacctgga gatcctgacc ggcaagcgca agtactacca
     2941 cggccccggc aacgagaaga gccgcaccga ctacggccgc taccgcacca gcggcgagac
     3001 cctggacaac atcttcggca tcctgatcgg caagtgcacc ttctaccccg acgagttccg
     3061 cgccgccaag gccagctaca ccgcccagga gttcaatctg ctgaacgacc tgaacaacct
     3121 gaccgtgccc accgagacca agaagctgag caaggagcag aagaaccaga tcatcaacta
     3181 cgtgaagaac gagaaggcta tgggccccgc caagctgttc aagtacatcg ccaagctgct
     3241 gagctgcgac gtggccgaca tcaagggcta ccgcatcgac aagagcggca aggccgagat
     3301 ccacaccttc gaggcctacc gcaagatgaa gaccctggag accctggaca tcgagcagat
     3361 ggaccgagag accctggaca agctggccta cgtgctgacc ctgaacaccg agcgcgaggg
     3421 catccaggag gccctggagc acgagttcgc cgacggcagc ttcagccaga aacaggtgga
     3481 cgagctggtg cagttccgca aggccaacag cagcatcttc ggcaagggct ggcacaactt
     3541 cagcgtgaag ctgatgatgg agctgatccc cgagctgtac gagaccagcg aggagcagat
     3601 gaccatcctg acccgcctgg gcaagcagaa gaccaccagc agcagcaaca agaccaagta
     3661 catcgacgag aagctgctga ccgaggagat ctacaacccc gtggtggcca agagcgtgcg
     3721 ccaggccatc aagatcgtga acgccgccat caaggagtac ggcgacttcg acaacatcgt
     3781 gatcgagatg gcccgcgaga ccaacgagga cgacgagaag aaggccatcc agaagatcca
     3841 gaaggccaac aaggacgaga aggacgccgc catgctgaag gccgccaacc agtacaacgg
     3901 caaggccgag ctgccccaca gcgtgttcca cggccacaag cagctggcca ccaagatccg
     3961 cctgtggcac cagcagggcg agcgctgcct gtacaccggc aagaccatca gcatccacga
     4021 cctgatcaac aacagcaacc agttcgaggt ggaccacatc ctgcccctga gcatcacctt
     4081 cgacgacagc ctggccaaca aggtgctggt gtacgccacc gccaaccagg agaagggcca
     4141 gcgcaccccc taccaggccc tggacagcat ggacgacgcc tggagcttcc gcgagctgaa
     4201 ggccttcgtg cgcgagagca agaccctgag caacaagaag aaggagtatc tgctgaccga
     4261 ggaggacatc agcaagttcg acgtgcgcaa gaagttcatc gagcgcaacc tggtggacac
     4321 ccgctacgcc agccgcgtgg tgctgaacgc cctgcaggag cacttccgcg cccacaagat
     4381 cgacaccaag gtgagcgtgg tgcgcggcca gttcaccagc cagctgcgcc gccactgggg
     4441 catcgagaag acccgcgaca cctaccacca ccacgccgtg gacgccctga tcattgcggc
     4501 ttctagccag ctgaacctgt ggaagaagca gaagaacacc ctggtgagct acagcgagga
     4561 gcagctgctg gacatcgaga ccggcgagct gatcagcgac gacgagtaca aggagagcgt
     4621 gttcaaggcc ccctaccagc acttcgtgga caccctgaag agcaaggagt tcgaggacag
     4681 catcctgttc agctaccagg tggacagcaa gttcaaccgc aagatcagcg acgccaccat
     4741 ctacgccacc cgccaggcca aggtgggcaa ggacaagaag gacgagacct acgtgctggg
     4801 caagatcaag gacatctaca cccaggacgg ctacgacgcc ttcatgaaga tctacaagaa
     4861 ggacaagagc aagttcctga tgtaccgcca cgacccccag accttcgaga aggtgatcga
     4921 gcccatcctg gagaactacc ccaacaagca gatgaacgag aaaggcaagg aggtgccctg
     4981 caaccccttc ctgaagtaca aggaggagca cggctacatc cgcaagtaca gcaagaaggg
     5041 caacggcccc gagatcaaga gcctgaagta ctacgacagc aagctgctgg gcaaccccat
     5101 cgacatcacc cccgaaaaca gcaagaacaa ggtggtgctg cagagcctta agccctggcg
     5161 caccgacgtg tacttcaaca agaacaccgg caagtacgag atcctggggc tgaagtacgc
     5221 cgatctgcag tttgagaaaa agacaggcac ctacaagatc agccaggaga agtacaacgg
     5281 catcatgaag gaggagggcg tggacagcga cagcgagttc aagttcaccc tgtacaagaa
     5341 cgaccttctg ctggtgaagg acaccgagac caaggagcaa cagctgttcc gcttcctgag
     5401 ccgcaccatg cccaacgtga agtactacgt ggagctgaag ccctacagca aggacaagtt
     5461 cgagaagaac gagagcctga tcgagatcct gggcagcgcc gacaagagcg gcaggtgcat
     5521 caagggcctg ggcaagagca acatcagcat ctacaaggtg cgcaccgacg tgctgggcaa
     5581 ccagcacatc atcaagaacg agggcgacaa gcccaagttg gacttcagca gggctgaccc
     5641 caagaagaag aggaaggtgg gatcctgata agaattcgcg gccgcactcc tcaggtgcag
     5701 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     5761 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     5821 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     5881 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     5941 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     6001 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     6061 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     6121 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     6181 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
     6241 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6301 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
     6421 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6481 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6541 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6601 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6661 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6721 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
     6781 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6841 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6901 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6961 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7021 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7081 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7141 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7201 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7261 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7321 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7381 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7441 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7501 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7561 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7621 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7681 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7741 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7801 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7861 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7921 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7981 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8041 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8101 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8161 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8221 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8281 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8341 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8401 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8461 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8521 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8581 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8641 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8701 aataggggtt ccgcgcacat ttccccgaaa agtgccacct g
//
