LOCUS       sequence-273389-        8469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM034Env
FEATURES             Location/Qualifiers
     source          1..8469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3967..4008
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4018..4035
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4058..4075)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4064..4288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4334..4762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4421..4440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4631..4652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4771..4791)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4776..5105
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4956..5091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5018..5037
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5172..5966
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5226..5245)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5836..5855
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6142..6263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6179..6198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6233..6252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6312..6328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6312..6328)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6325..6347)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6336..6352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6360..6366,6367..6384,6385..6390))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6405..6426
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6543..6560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6714..7302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6794..6813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7473..8264,8265..8333))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8096..8115
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8334..8438)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccagtac ccttcaccga tcaagcttta ggcatctcct
      961 atggcaggaa gaagcggaag caccgacgaa gaactcctcg gagctgtgag gatcatcaaa
     1021 atcctctatc aaagcagtaa gtaataagta tatgtaatga tacctttgga aattagtgca
     1081 atagtaggac tgatagtagc gctaatctta gcaatagtag tgtggactat agtagctata
     1141 gaacttaaga aaatactaga gcagagaaaa atagacaggt taattaagag aataagagaa
     1201 agagcagaag acagtggaaa tgagagtgag ggagacacag atgaattggc caaacttgtg
     1261 gagatgggga actttgatcc ttgggttggt gataatttgt agtgcctcag acaacttgtg
     1321 ggttacagtt tattatgggg ttcctgtgtg gagagatgca gataccaccc tattctgcgc
     1381 atcagatgcc aaagcacatg acacagaagt gcacaatgtc tgggccacat atgcttgtgt
     1441 acccacagac cccaacccac aagaaataca cctggaaaat gtaacagaaa attttaacat
     1501 gtggaaaaat aacatggtag agcagatgca ggaggatgta atcagtttat gggatcaaag
     1561 tctaaagcca tgtgtaaagt taactcctct ctgcgtcact ttaacttgta ctactgctaa
     1621 tttgaacaat accactgaag cccataacaa ctcaacagga aatatgatag gaaatctgac
     1681 agatgaagta agaaactgtt cttttaatat gaccacagaa ataagagata agaagcagaa
     1741 ggcctatgca cttttttata agcttgatat agtaccaata catggggacg atatcacgta
     1801 taggttaata aattgtaata cttcagtcat taagcaggct tgtccaaagg tatcctttga
     1861 tccaattcct atacattatt gtactccagc tggttatgcg attttaaagt gtaatgataa
     1921 aaaattcaat gggacagggc cgtgtaaaaa tgttagctca gtacaatgca cacatggaat
     1981 taagccagtg gtatcaactc aactactgtt aaatggcagt ctagcagaag aagagataat
     2041 aatcagatct gaaaatatca cagacaatac caaaaacata atagtgcacc ttaatcagac
     2101 tgtagaaatc aattgtacca gacccaacaa caatacaata acaagcttaa ctataggacc
     2161 aggacaagtg ttctatagaa caggagacat aaaaggagat ataagaaaag catattgtga
     2221 gattaatgca acaaaatggt ataaagtttt aaaacaggta acagaaaaat taaaagaaca
     2281 cgttaataag acaataaact ttaaaccacc ttcaggagga gatctagaaa ttacaacgca
     2341 tcattttaat tgtagagggg aatttttcta ttgcaataca acacaactgt ttaataatac
     2401 acaccgggta aatgatactg gcataaacgg gacgatcata cttccatgca ggataaaaca
     2461 aatcataaac atgtggcagg gagtaggaca agcaatgtat gctcctccca tcagtggaag
     2521 aattaattgt gtatcaaata ttacaggaat actattgaca agtgatggtg atgaaaatgc
     2581 caccttcaga ccggggggag gagatataaa ggacaattgg agaagtgaat tatataaata
     2641 taaagtagta caaattgaac cactaggaat agcacccacc agggcaaaga gaagagtggt
     2701 ggagagagaa aaaagagcag tgggaatagg agctatgatc tttgggttct tgggagcagc
     2761 aggaagcact atgggcgcgg cgtcaataac gctgacggta caagccagac aattattgtc
     2821 gggtatagtg caacagcaaa gcaatttgct gagggctata gaggcgcagc agcatctgtt
     2881 gcaactcaca gtctggggca ttaaacagct ccaggcaaga gtcctggctg tggaaagata
     2941 cctaaaagat cagacgttcc taggactttg gggctgctct ggaaaaatca tctgcaccac
     3001 taatgtgccc tggaactcca cttggagtaa taaatctttt aaagagattt gggataacat
     3061 gacatggata gaatgggaga gagaaattag caattacaca aaccaaatat atgagatact
     3121 tacagaatcg cagaaccagc aggacagaaa tgaaaaggag ttattagaat tggataagtg
     3181 ggcaagtctg tggaattggt ttgacataac aaattggctg tggtatataa aaatatttat
     3241 aatgatagta ggaggcttaa taggtttaag aataattttt gctgtacttt ctatagtgaa
     3301 tagagttagg cagggatact cacctttgtc gttccagacc cctacccatc atcagaggga
     3361 acccgacaga cccgaaagaa tcgaagaagg aggtggcgag caaggcagag acagatccgt
     3421 gagattagtg agcgggttct tagcacttgt ctgggacgat ctacggagcc tgtgcctctt
     3481 cagctaccac cgcttgagag acttcggctt gattgcagcg aggactgtgg aaattctggg
     3541 acacagcagt ctcaagggac tgagacgggg gtgggaagcc ctcaaatatc tggggaatct
     3601 tctgttatat tggggtcagg aacaaaaaat tagtgctatt tccttgctta atgctacagc
     3661 aatagtagta gcggagggga cagatagggt tatagaaata gtacaaagag cttggagagc
     3721 ctttctccac atacctagaa gaatcagaca gggcttagaa aggactttgc tataatatgg
     3781 gaggcaagtg gtcaaaaagt agcatagtgg gatggcctca ggtcagggaa aggataaagc
     3841 aaacccctcc agcagcagaa ggagtaggag cagtatctca agacggatcc agctaagggt
     3901 caagacaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
     3961 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
     4021 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     4081 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4141 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4201 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4261 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     4321 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4381 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4441 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4501 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4561 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4621 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4681 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4741 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4801 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4861 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4921 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4981 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5041 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5101 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     5161 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5221 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5281 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5341 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5401 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5461 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5521 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5581 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5641 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5701 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5761 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5821 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5881 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5941 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa
     6001 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6061 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6121 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6241 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     6601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     6661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7141 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     7621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     7681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8461 cctgacgtc
//
