LOCUS       sequence-273400-        3572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr536.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr536
FEATURES             Location/Qualifiers
     source          1..3572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     138..157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     391..408
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    525..546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(561..566,567..584,585..591)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    599..615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     604..626
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     623..639
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..639
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        657..675
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     657..674
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1326..1335
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1330..1339
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1369..1388)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1370..1388)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1395..1412)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1395..1411)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1404..1426)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1552..2007
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1639..1658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1849..1870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2085..2189
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2190..2258,2259..3050)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2408..2427)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3221..3572,1..237)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
       61 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      121 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      181 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      241 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      301 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      361 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      421 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      481 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      541 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      601 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctattt
      661 aggtgacact atagaatact caagctatgc atccaacgcg ttgggagctc tcccatatgg
      721 tcgacctgca ggcggccgcg aattcactag tgatttttcc ccgttgatga ctttgagctt
      781 ggagaagagc tggcaaggag ctcaagggat cactgaaacc ttcctttctg taacatctga
      841 ctccggaggg cacaggctaa gttatagcct gggacagctg tccaaagtct gtatacagaa
      901 aatcatgctg tctacaagat gttttagtaa gctttagcca tttctacttc atacatagat
      961 gtgtgtgctt ataagttcaa tgctcttaaa tgttaagatg cttctatgac tgaaccaaat
     1021 gtagttaaat attgagtata gtccttttaa tgacctattt aaaattccct gatcctctgt
     1081 gtcactggct gcaagtgaat gcttatagag ataaagaaac atgactccct ctgctcccac
     1141 ccccgccata cacacatctg ctctgagcat ttgctggcct gatttggctg ttttcatctt
     1201 tcttattctt atctcacagt ttacactggt gcttggatca tttaatagtt gaaggaagga
     1261 aactaataaa caaactgagt tagggctggg tatgggggcc taggatctct aatcgaattc
     1321 ccgcggccgc catggcggcc gggagcatgc gacgtcgggc ccaattcgcc ctatagtgag
     1381 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1441 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1501 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct
     1561 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     1621 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     1681 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     1741 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     1801 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     1861 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     1921 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     1981 ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg
     2041 cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2101 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2161 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2221 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2281 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2341 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2401 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2461 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2521 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2581 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2641 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2701 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2761 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2821 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2881 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2941 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3001 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3061 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3121 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3181 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3241 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3301 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3361 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3421 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3481 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3541 cggggggttc gtgcacacag cccagcttgg ag
//
