LOCUS       sequence-273473-        3937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr686.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr686
FEATURES             Location/Qualifiers
     source          1..3937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     90..112
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     104..121
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     105..121
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     128..147
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        128..146
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      177..186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      181..190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1206..1224)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1207..1224)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1242..1258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1242..1258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1255..1277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1266..1282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1290..1296,1297..1314,1315..1320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1335..1356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1473..1490)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1644..2232)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1724..1743)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2403..3194,3195..3263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3026..3045
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3264..3368)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3446..3901)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3583..3604)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3795..3814
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
       61 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      121 tgaattgtaa tacgactcac tatagggcga attgggcccg acgtcgcatg ctcccggccg
      181 ccatggcggc cgcgggaatt cgattccaca tactttcttc ttgactggca tcccaggact
      241 gactgctgcc catgtttgga tatcccttcc cttctgcttc atgtttgtcc tgtcactaac
      301 tgggaatgca gtcctgcttt ctctcatctg gatagaacac aggctccacc agcccatgtt
      361 tttgtttctt gcaatgctct cttttgttga tctggtcctc tccctttcca ctctgcccaa
      421 gatgctggct attttctggt ttggtgccac agccatcagc tcctatgcct gtctgtccca
      481 aatgttcctc atccatgcat tctctgccat ggagtcaggg gtgctggtgg ccatggcctt
      541 ggaccgattt gtggctatct gtaacccatt gcattatgca accatcctta cgcctgaagt
      601 tgttgccaag attggaggcc tggtggcact gcgaggtgtg ggtttaacta tcttctttcc
      661 aagcctggct tgtcgactgt cttattgtgg ttcacacaca attgcttaca cttactgtga
      721 acacatgtcc gtggtgaagc tggcttgtgg ggccataact gtggacagtc tctatgcttt
      781 tgctgtggca atctttcttg gtgtggggga tatggccttt attgcctact cttatggaca
      841 gattgtgaag acagtgatgc gttttccttc tccagaggca cgtggcaaag caggaagtac
      901 atgcacagcc catgtctgtg tcatcctctt cttctatgga cctggctttc tttctgtggt
      961 catgcagcgc tttgggccat ccacagcttc tgctgccaag gtcatccttg ccaatctcta
     1021 cttgcttttt ccccctgctt tggaccccat tgtatatggg gttaagacca aacagatcag
     1081 agagtgtctg ttcacaatta taggttccaa gaagattgaa cccacaatca ctagtgaatt
     1141 cgcggccgcc tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg
     1201 agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1261 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1321 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1381 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1441 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1501 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1561 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1621 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1681 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1741 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1801 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1861 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1921 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1981 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2041 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2101 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2161 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2221 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2281 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2341 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2401 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2461 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2521 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2581 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2641 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2701 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2761 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2821 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2881 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2941 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3001 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3061 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3361 gttccgcgca catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg
     3421 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     3481 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     3541 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     3601 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     3661 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     3721 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     3781 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     3841 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     3901 tccattcgcc attcaggctg cgcaactgtt gggaagg
//
