LOCUS       sequence-273523-        9563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-tagged peroxisome marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-PTS1-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..9563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             73..706
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    753..878
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1375..1608
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1793..1837
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2100..2215
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2409..3591
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2640..3582
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3539..3559
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3635..3644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3641..3643,3644..3646,3647..4357)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3641..3643,3644..3646,3647..4357)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3686..3707)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3947..3966)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4294..4315
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4385..4959,4960..4962,4963..4971)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4552..4569)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4779..4798
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4991..5590
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4991..5010)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5487..5507
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5604..6192
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5657..5677)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6075..6086)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6399..7032
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7160..7176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7160..7176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7173..7195)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7184..7200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7208..7214,7215..7232,7233..7238))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7253..7274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7391..7408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7562..8150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7642..7661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8321..9112,9113..9181))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8944..8963
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9182..9286)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9334..9468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9371..9390)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9425..9444
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
       61 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
      121 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
      181 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
      241 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
      301 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
      361 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
      421 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
      481 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
      541 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      601 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      661 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      721 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      781 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
      841 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
      901 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
      961 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1021 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1081 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1141 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1201 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1261 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1321 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1381 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1441 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1501 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1561 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1621 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1681 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1741 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1801 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1861 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1921 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1981 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2041 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcct
     2101 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2161 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2221 tttattacag ggacagcaga gatccagttt atcgatgagt aattcataca aaaggactcg
     2281 cccctgcctt ggggaatccc agggaccgtc gttaaactcc cactaacgta gaacccagag
     2341 atcgctgcgt tcccgccccc tcacccgccc gctctcgtca tcactgaggt ggagaagagc
     2401 atgcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2461 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2641 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     2701 cttgaattac ttccacgccc ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     2761 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     2821 gttgaggcct ggcttgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     2881 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     2941 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     3001 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     3061 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     3121 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     3181 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     3241 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     3301 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     3361 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     3421 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     3481 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     3541 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggatctatt
     3601 tccggtgaat tcctcgagac tagcgctacc ggtcgccacc atggtgagca agggcgagga
     3661 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3721 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3781 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     3841 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3901 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3961 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4021 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4081 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4141 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4201 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4261 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4321 cgccgggatc actctcggca tggacgagct gtacaagtcc aagctgtagc ggccgcggat
     4381 cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4441 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4501 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4561 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4621 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4681 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4741 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4801 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4861 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4921 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag
     4981 acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5041 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5101 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5161 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5221 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5281 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5341 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     5401 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5461 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5521 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     5581 cggtgcctag acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
     5641 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5701 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5761 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5821 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5881 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5941 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     6001 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     6061 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     6121 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     6181 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
     6241 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6301 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6361 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca
     6421 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     6481 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     6541 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     6601 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     6661 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     6721 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     6781 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     6841 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6901 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6961 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7021 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     7081 aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct
     7141 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7201 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7261 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7321 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7381 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7981 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     8041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8221 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8281 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8341 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8401 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8461 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8521 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8581 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8641 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8701 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8761 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8821 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8881 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8941 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9001 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9061 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9121 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9181 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9241 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9301 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
     9361 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     9421 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     9481 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     9541 ctgtggtttc atttactcta aac
//
