LOCUS       sequence-273532-        5592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5592)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-024   Lamp1-Neptune//BsaI
FEATURES             Location/Qualifiers
     source          1..5592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2976..3097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3013..3032)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3067..3086
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      3898..3927
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3949..4536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4437..4456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4548..4575)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4599..5390,5391..5459))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5222..5241
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5460..5562)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5563..5592,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggs ggggsgrggg gsggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tggccgcacc cggtgcaaga cgccctcttc tgctgctgct gctggccggc ctcgctcact
      901 cagctcccgc actctttgag gtgaaagaca acaacggaac tgcgtgtatt atggcatctt
      961 tcagcgcttc attcctgaca acgtatgaag cggggcatgt cagtaaagtg agcaacatga
     1021 ctctgccagc cagtgctgaa gtgctgaaga actccagctc atgcggggaa aagaacgcct
     1081 cagaaccaac cctcgctata acctttgggg agggctactt gttgaagttg acgtttacga
     1141 agaacacaac acggtattcc gtacagcaca tgtacttcac ttacaacctg agcgatactc
     1201 agttctttcc caatgcaagt tccaagggcc cagacactgt tgattccacc accgacatca
     1261 aggccgacat caacaagaca tacagatgcg tgtctgacat ccgggtgtac atgaagaatg
     1321 tcactattgt actctgggat gccaccatac aggcctattt gcccagcagc aatttcagca
     1381 aagaggaaac aaggtgtccg caggatcagc catctcctac cactggacct cctagtccat
     1441 ccccaccgct tgtccctaca aaccccagcg tgagcaaata caacgtcaca ggagacaatg
     1501 gcacctgtct gctggcatcc atggctttgc agctgaatat cacctatatg aagaaggaca
     1561 atacgaccgt gactcgagcc tttaacatca atccttctga caaatacagc ggtacctgtg
     1621 gggctcaact ggtgacactc aaagtcggta acaaatctcg cgtgctggaa ctgcagtttg
     1681 gcatgaatgc cacctcctct ctgttcttcc tgcaaggtgt gcagctgaat atgacccttc
     1741 ctgatgccat tgagcccaca ttctccacca gcaactatag tctgaaggcg cttcaagcta
     1801 gcgttgggaa cagttacaaa tgcaattctg aggagcacat tttcgtgtca aaggcccttg
     1861 ctctcaatgt cttttccgtt caggtacagg cattcagagt ggagtctgat cgtttcggct
     1921 cagtagaaga gtgcgtccaa gacggcaata acatgctgat ccccattgcc gttggaggag
     1981 ctcttgccgg actggttctc atcgtgctca ttgcctacct gataggcagg aagaggtcac
     2041 atgcagggta tcagacaatc agcgagtttg ggagtacagg atccactggt tctactggcg
     2101 ctgatggcgg gtcaggcggc agcgtcagca aaggcgagga gctcatcaag gagaacatgc
     2161 acaccaagct gtacatggaa ggtactgtga ataaccacca cttcaaatgc acgcatgaag
     2221 gtgaaggcaa gccctatgag ggcacacaga ccaatcgcat caaagtggtt gagggaggtc
     2281 ctctgccgtt tgcgttcgac attctggcca catgcttcat gtatggctcc aaaacgttca
     2341 tcaaccacac tcagggcata cccgatttct tcaagcagtc ttttcctgaa ggctttacat
     2401 gggagagagt gaccacatac gaagatggag gcgtcctcac tgtgacacaa gacacaagtc
     2461 tgcaggatgg atgtctcatc tacaatgtga agctgcgtgg ggtgaacttt ccctctaatg
     2521 ggcctgtaat gcagaagaaa actcttgggt gggaagcatc aaccgagact ctttacccag
     2581 ctgatggagg gcttgaaggc cggtgtgaca tggctctcaa gttggtaggt ggaggacatc
     2641 tgcattgcaa cctgaaaacc acctacagga gcaagaagcc agcaaagaac ctgaagatgc
     2701 caggagtcta ttttgtggac agacgattgg agcggattaa ggaggccgac aatgaaacct
     2761 atgtcgagca acacgaggtt gccgttgcta ggtattgtga cttgccttcc aaactggggc
     2821 ataagctgaa cgggatggat gagctgtaca aatcaggctc tgattaaggc gccaggtaca
     2881 tctccaatac ggcgtcttgc atagagaaca cgattagcac accggttacc cacgtgatgc
     2941 ccgaacggac ccatgtcatg ccagagcgca ctggtaactt gtttattgca gcttataatg
     3001 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3061 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtcgt cgaattcgag
     3121 ttggcgcgcc tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc
     3181 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     3241 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc
     3301 ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt
     3361 acatccctgg gggctttggg agggggctgt ccccgtgagc tcccaggcgc gcctgtcatt
     3421 ctaaatctct ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc
     3481 aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg
     3541 ctgtccccgc acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc
     3601 cgggcggctc gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt
     3661 gggggggggc tgtccccgtg agctcccagg cgcgccaact cggtacctca cccgattctc
     3721 atcatggaga cacatcagga catctccaat accgccatgg aaacgcacca agatgcgact
     3781 gctcttctgc cagtgacggc attcgtctgc aagattaacg gcatacagaa agaaacgatg
     3841 agacccattc aggacgagcc tcagactcca gcgtaactgg actgcaatca actcactggc
     3901 tcaccttcac gggtgggcct ttcttcggta gaaaatcaaa ggatcttctt gagatccttt
     3961 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4021 tttgccggat caagagctac caactctttt tccgaggtaa ctggcttcag cagagcgcag
     4081 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcatcgattt
     4501 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc agaaaggccc
     4561 acccgaaggt gagccaggtg attacatttg ggccctcatt accaatgctt aatcagtgag
     4621 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4681 tagataacta cgatgcggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4741 gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg gagggccgag
     4801 cgcagaagtg atcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4861 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4921 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4981 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5041 atcgttgcca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5101 aattctctta ctgtcatgcc atccgtgaga tgcttttctg tgactggtga gtactcaacc
     5161 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5221 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5281 gggcgtaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5341 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5401 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcatt
     5461 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     5521 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caagtcaaaa gcctccggtc
     5581 ggaggctttt ga
//
