LOCUS       sequence-273587-       10835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP-tagged lysosomal marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 10835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..10835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        71..1253
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          302..1244
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1201..1221
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(1281..1364,1365..2531)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     CDS             join(2571..2573,2574..2576,2577..3290)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2571..2573,2574..2576,2577..3287)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2616..2637)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2877..2896)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3224..3245
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3319..3893,3894..3896,3897..3905)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3486..3503)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3713..3732
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3925..4524
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3925..3944)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4421..4441
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4538..5126
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4591..4611)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5009..5020)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5333..5966
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6094..6110)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6094..6110)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6107..6129)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6118..6134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6142..6148,6149..6166,6167..6172))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6187..6208
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6325..6342)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6496..7084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6576..6595)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7255..8046,8047..8115))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7878..7897
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8116..8220)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8268..8402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8305..8324)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8359..8378
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8570..9203
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9250..9375
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9872..10105
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10290..10334
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10597..10712
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag gtggagaaga
       61 gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
      121 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
      181 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
      241 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
      301 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
      361 gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc gagcttcggg
      421 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
      481 gagttgaggc ctggcttggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
      541 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
      601 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
      661 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
      721 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
      781 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
      841 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
      901 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
      961 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     1021 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     1081 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     1141 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     1201 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggatcta
     1261 tttccggtga attcagggac atggcggccc ccggcagcgc ccggcgaccc ctgctgctgc
     1321 tactgctgtt gctgctgctc ggcctcatgc attgtgcgtc agcagcaatg tttatggtga
     1381 aaaatggcaa cgggaccgcg tgcataatgg ccaacttctc tgctgccttc tcagtgaact
     1441 acgacaccaa gagtggccct aagaacatga cctttgacct gccatcagat gccacagtgg
     1501 tgctcaaccg cagctcctgt ggaaaagaga acacttctga ccccagtctc gtgattgctt
     1561 ttggaagagg acatacactc actctcaatt tcacgagaaa tgcaacacgt tacagcgtcc
     1621 agctcatgag ttttgtttat aacttgtcag acacacacct tttccccaat gcgagctcca
     1681 aagaaatcaa gactgtggaa tctataactg acatcagggc agatatagat aaaaaataca
     1741 gatgtgttag tggcacccag gtccacatga acaacgtgac cgtaacgctc catgatgcca
     1801 ccatccaggc gtacctttcc aacagcagct tcagcagggg agagacacgc tgtgaacaag
     1861 acaggccttc cccaaccaca gcgccccctg cgccacccag cccctcgccc tcacccgtgc
     1921 ccaagagccc ctctgtggac aagtacaacg tgagcggcac caacgggacc tgcctgctgg
     1981 ccagcatggg gctgcagctg aacctcacct atgagaggaa ggacaacacg acggtgacaa
     2041 ggcttctcaa catcaacccc aacaagacct cggccagcgg gagctgcggc gcccacctgg
     2101 tgactctgga gctgcacagc gagggcacca ccgtcctgct cttccagttc gggatgaatg
     2161 caagttctag ccggtttttc ctacaaggaa tccagttgaa tacaattctt cctgacgcca
     2221 gagaccccgc ctttaaagct gccaacggct ccctgcgagc gctgcaggcc acagtcggca
     2281 attcctacaa gtgcaacgcg gaggagcacg tccgtgtcac gaaggcgttt tcagtcaata
     2341 tattcaaagt gtgggtccag gctttcaagg tggaaggtgg ccagtttggc tctgtggagg
     2401 agtgtctgct ggacgagaac agcatgctga tccccatcgc tgtgggtggt gccctggcgg
     2461 ggctggtcct catcgtcctc atcgcctacc tcgtcggcag gaagaggagt cacgcaggct
     2521 accagactat cggctccacc ggctccacag gctccaccgg cgcggtcgac atggtgagca
     2581 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2641 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2701 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2761 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2821 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     2881 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     2941 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3001 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     3061 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3121 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3181 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3241 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtga actagttcta
     3301 gagcggccgc ggatcccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     3361 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     3421 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     3481 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     3541 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3601 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3661 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3721 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3781 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     3841 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca
     3901 caacccacaa ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc
     3961 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4021 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4081 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4141 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4201 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4261 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     4321 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     4381 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     4441 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     4501 atgacccgca agcccggtgc ctagacgcgt ctggaacaat caacctctgg attacaaaat
     4561 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4621 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4681 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4741 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4801 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4861 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4921 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4981 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5041 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5101 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
     5161 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     5221 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     5281 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
     5341 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     5401 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     5461 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     5521 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     5581 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     5641 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     5701 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     5761 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     5821 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5881 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5941 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6001 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc
     6061 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6121 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6181 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6241 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6301 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6361 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6421 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6481 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6541 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6601 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6661 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6721 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6781 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6841 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6901 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6961 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7021 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7081 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7141 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7201 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7261 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7321 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7381 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7441 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7501 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7561 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7621 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7681 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7741 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7801 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7861 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7921 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7981 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8041 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8101 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8161 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8221 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
     8281 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8341 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     8401 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     8461 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     8521 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     8581 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8641 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     8701 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     8761 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     8821 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     8881 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     8941 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     9001 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9061 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9121 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9181 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     9241 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     9301 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     9361 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     9421 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     9481 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     9541 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     9601 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     9661 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     9721 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9781 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9841 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9901 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9961 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    10021 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
    10081 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
    10141 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
    10201 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
    10261 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10321 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10381 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10441 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
    10501 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
    10561 ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg
    10621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    10681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    10741 ccagtttatc gatgagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg
    10801 gaccgtcgtt aaactcccac taacgtagaa cccag
//
