LOCUS       sequence-273751-       11290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  destabilized GFP reporter to monitor calcineurin activity via 
            calcineurin-dependent response element (CDRE) in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11290)
  AUTHORS   Diessl , J.,  Nandy, A., Schug, C., Habernig, L.,, Buttner, S.
  TITLE     Stable and destabilized GFP reporters to monitor calcineurin 
            activity in Saccharomyces cerevisiae.
  JOURNAL   Microbial Cell, 2020: in press
REFERENCE   2  (bases 1 to 11290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbial 
            Cell, 2020: in press"
COMMENT     Sequence Label: pAMS366-4xCDRE-GFP-PEST
FEATURES             Location/Qualifiers
     source          1..11290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        290..505
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             506..1309
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             1781..2494
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(7055..7077)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7163..7303
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7218..7237
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7318..7335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7478..8066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7558..7577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8237..9028,9029..9097))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8860..8879
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9098..9202)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9270..9288
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      9646..10988
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    9978..10025
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tgagaagctg cagcgtcaca tcggataata atgatggcag ccattgtaga agtgcctttt
       61 gcatttctag tctctttctc ggtctagcta gttttactac atcgcgaaga tagaatctta
      121 gatcacactg cctttgctga gctggatcaa tagagtaaca aaagagtggt aaggcctcgt
      181 taaaggacaa ggacctgagc ggaagtgtat cgtacagtag acggagtata ctagtatagt
      241 ctatagtccg tggaattctc atgtttgaca gcttatcatc gataagcttt tcaattcatc
      301 attttttttt tattcttttt tttgatttcg gtttccttga aatttttttg attcggtaat
      361 ctccgaacag aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat
      421 atgtagtgtt gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa
      481 aacctgcagg aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac
      541 tcatcctagt cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt
      601 gtgtgcttca ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg
      661 tcccaaaatt tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg
      721 cacagttaag ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag
      781 aaaatttgct gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat
      841 agcagaatgg gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg
      901 tttgaagcag gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga
      961 attgtcatgc aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc
     1021 gaagagcgac aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga
     1081 tgaaggttac gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc
     1141 attgggtcaa cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat
     1201 tgttggaaga ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag
     1261 aaaagcaggc tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt
     1321 ataagtaaat gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt
     1381 attaccctcg agcaagcgca cagccaccga ctggtgtcga gcaagcgcac agccaccgac
     1441 tggtgtcgag gtcgagcaag cgcacagcca ccgactggtg tcgaggtcga gcaagcgcac
     1501 agccaccgac tggtgtcgag gtcgagcaga tccgccaggc gtgtatatag cgtggatggc
     1561 caggcaactt tagtgctgac acatacaggc atatatatat gtgtgcgacg acacatgatc
     1621 atatggcatg catgtgctct gtatgtatat aaaactcttg ttttcttctt ttctctaaat
     1681 attctttcct tatacattag gtcctttgta gcataaatta ctatacttct atagacacgc
     1741 aaacacaaat acacacacta aattaataat gaccggatcc atgtctaaag gtgaagaatt
     1801 attcactggt gttgtcccaa ttttggttga attagatggt gatgttaatg gtcacaaatt
     1861 ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt aaattgacct taaaatttat
     1921 ttgtactact ggtaaattgc cagttccatg gccaacctta gtcactactt tcggttatgg
     1981 tgttcaatgt tttgcgagat acccagatca tatgaaacaa catgactttt tcaagtctgc
     2041 catgccagaa ggttatgttc aagaaagaac tatttttttc aaagatgacg gtaactacaa
     2101 gaccagagct gaagtcaagt ttgaaggtga taccttagtt aatagaatcg aattaaaagg
     2161 tattgatttt aaagaagatg gtaacatttt aggtcacaaa ttggaataca actataactc
     2221 tcacaatgtt tacatcatgg ctgacaaaca aaagaatggt atcaaagtta acttcaaaat
     2281 tagacacaac attgaagatg gttctgttca attagctgac cattatcaac aaaatactcc
     2341 aattggtgat ggtccagtct tgttaccaga caaccattac ttatccactc aatctgcctt
     2401 atccaaagat ccaaacgaaa agagagacca catggtcttg ttagaatttg ttactgctgc
     2461 tggtattacc catggtatgg atgaattgta caaagcatcc aacttgaaca tttcgagaaa
     2521 gcttaccata tcaaccccat catgctcttt cgaaaattca aatagcacat ccattccttc
     2581 gcccgcttcc tcatctcaaa gccacactcc aatgagaaac atgagctcac tctctgataa
     2641 cagcgttttc agccggaata tggaacaatc atcaccaatc actccaagta tgtaccaatt
     2701 tggtcagcag cagtcaaaca gtatatgtgg tagcaccgtt agtgtgaata gtctggtgaa
     2761 tacaaataac aaacaaagga tctacgaaca aatcacgggt cctaacagca ataacgcaac
     2821 caatgattat attgatttgc taaacctaaa tgagtctaac aaggaaaacc aaaatcccgc
     2881 aacggcgcat tacctcaatg ggggcccacc caagacaagc ttcattaacc atggaatgtt
     2941 cccctcgcca actgggacca taaatagcgg taaatctagc agtgcctcat ctttaatttc
     3001 ttttggtatg ggcaataccc aagtaatata gggatccgga gcttggctgt tgcccgtctc
     3061 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     3121 ggccgattca ttaatgcagc tggcacgaca ggtttcccga cttaatcgcc ttgcagcaca
     3181 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     3241 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc
     3301 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg
     3361 gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa
     3421 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga
     3481 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt
     3541 tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga
     3601 atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg
     3661 ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg
     3721 tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg
     3781 ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt
     3841 gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg
     3901 caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac
     3961 actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg
     4021 tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt
     4081 cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct
     4141 gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca
     4201 gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc
     4261 gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt
     4321 ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc
     4381 gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca
     4441 cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc
     4501 gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg
     4561 agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca
     4621 gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac
     4681 gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc
     4741 taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg
     4801 ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg
     4861 tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga
     4921 ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg
     4981 tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga
     5041 gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct
     5101 ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag
     5161 cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga
     5221 agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct
     5281 ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga
     5341 gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat
     5401 tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga
     5461 taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg
     5521 gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact
     5581 tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt
     5641 tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt
     5701 tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca
     5761 ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct
     5821 tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt
     5881 cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca
     5941 gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac
     6001 cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca
     6061 tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat tccagctgag
     6121 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc
     6181 atgtctgccc gtatttcgcg taaggaaatc cattgtactg ccggaccacc gactgtgagc
     6241 cactccggcc atggcgtacg cactgacctg cttactgatt tgtaaaaccg gtccggccat
     6301 cacgctcaca taacgtccac gcaggctctc atagtgaaac gtatcctccc cggtcatcac
     6361 tgtgctgctc tttttcgacg cggcgaaccc cagggaagcc atcaccccca cactgtccgt
     6421 cagctcataa cggtacttca cgttaatccc tttcagatga ctcacaccgg tatccccgcc
     6481 cgacaacgac ggcaatgtac ccggtttcac ttgaaaatag cccaccgtaa acgtaccatg
     6541 tccaccttcc gcacgggccg gagtgactgt caccgcaagt gcggcaaaga cagcaacggc
     6601 aatacacaca ttacgcatcg ttcacctctc actgttttat aataaaacgc ccgttcccgg
     6661 acgaacctct gtaacacact cagaccacgc tgatgcccag cgcctgtttc ttaatcacca
     6721 taacctgcac atcgctggca aacgtatacg gcggaatatc tgccgaatgc cgtgtggacg
     6781 taagcgtgaa cgtcaggatc acgtttcccc gacccgctgg catgtcaaca atacgggaga
     6841 acacctgtac cgcctcgttc gccgcgccat cataaatcac cgcaccgttc atcagtactt
     6901 tcagataaca catcgaatac gttgtcctgc cgctgacagt acgcttactt ccgcgaaacg
     6961 tcagcggaag caccactatc tggcgatcaa aaggatggtc atcggtcacg gtgacagtac
     7021 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7081 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7141 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     7201 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     7261 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     7321 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7381 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7441 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7501 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7561 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7621 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7681 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7741 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7801 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7861 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7921 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7981 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8041 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gtgtctgacg
     8101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8161 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8221 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8341 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8401 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8461 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8521 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     8581 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8641 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8701 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8761 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8821 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     8881 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8941 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9001 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9061 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9121 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9181 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9241 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     9301 aagaattctg aaccagtcct aaaacgagta aataggaccg gcaattcttc aagcaataaa
     9361 caggaatacc aattattaaa agataactta gtcagatcgt acaataaagc tttgaagaaa
     9421 aatgcgcctt attcaatctt tgctataaaa aatggcccaa aatctcacat tggaagacat
     9481 ttgatgacct catttctttc aatgaagggc ctaacggagt tgactaatgt tgtgggaaat
     9541 tggagcgata agcgtgcttc tgccgtggcc aggacaacgt atactcatca gataacagca
     9601 atacctgatc actacttcgc actagtttct cggtactatg catatgatcc aatatcaaag
     9661 gaaatgatag cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag
     9721 ctaaagggta gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag
     9781 gaggtactag actacctttc atcctacata aatagacgca tataagtacg catttaagca
     9841 taaacacgca ctatgccgtt cttctcatgt atatatatat acaggcaaca cgcagatata
     9901 ggtgcgacgt gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc ggaagcgctc
     9961 gttttcggaa acgctttgaa gttcctattc cgaagttcct attctctaga aagtatagga
    10021 acttcagagc gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa
    10081 acgcaccgga ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct
    10141 caaaagtatc tctttgctat atatctctgt gctatatccc tatataacct acccatccac
    10201 ctttcgctcc ttgaacttgc atctaaactc gacctctaca ttttttatgt ttatctctag
    10261 tattactctt tagacaaaaa aattgtagta agaactattc atagagtgaa tcgaaaacaa
    10321 tacgaaaatg taaacatttc ctatacgtag tatatagaga caaaatagaa gaaaccgttc
    10381 ataattttct gaccaatgaa gaatcatcaa cgctatcact ttctgttcac aaagtatgcg
    10441 caatccacat cggtatagaa tataatcggg gatgccttta tcttgaaaaa atgcacccgc
    10501 agcttcgcta gtaatcagta aacgcgggaa gtggagtcag gcttttttta tggaagagaa
    10561 aatagacacc aaagtagcct tcttctaacc ttaacggacc tacagtgcaa aaagttatca
    10621 agagactgca ttatagagcg cacaaaggag aaaaaaagta atctaagatg ctttgttaga
    10681 aaaatagcgc tctcgggatg catttttgta gaacaaaaaa gaagtataga ttctttgttg
    10741 gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt
    10801 ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca aaaatgaagc acagattctt
    10861 cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg taaaaatgca gctcagattc
    10921 tttgtttgaa aaattagcgc tctcgcgttg catttttgtt ctacaaaatg aagcacagat
    10981 gcttcgttaa caaagatatg ctattgaagt gcaagatgga aacgcagaaa atgaaccggg
    11041 gatgcgacgt gcaagattac ctatgcaata gatgcaatag tttctccagg aaccgaaata
    11101 catacattgt cttccgtaaa gcgctagact atatattatt atacaggttc aaatatacta
    11161 tctgtttcag ggaaaactcc caggttcgga tgttcaaaat tcaatgatgg gtaacaagta
    11221 cgatcgtaaa tctgtaaaac agtttgtcgg atattaggct gtatctcctc aaagcgtatt
    11281 cgaatatcat
//
