LOCUS       sequence-27377-e         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Charpentier X, Gabay JE, Reyes M, Zhu JW, Weiss A, Shuman HA
  TITLE     Chemical genetics reveals bacterial and host cell functions critical
            for type IV effector translocation by Legionella pneumophila.
  JOURNAL   PLoS Pathog. 2009 Jul . 5(7):e1000501.
  PUBMED    19578436
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog. 2009 Jul . 5(7):e1000501."
COMMENT     Sequence Label: pXDC61
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             123..501
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(157..176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tatggctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
       61 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      121 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      181 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      241 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      301 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      361 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      421 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      481 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      541 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      601 ggcccttccg gctggctggt tt
//
