LOCUS       sequence-273794-       11631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Medina EM, Robinson KA, Bellingham-Johnstun K, Ianiri G, Laplante C,
            Fritz-Laylin LK, Buchler NE
  TITLE     Genetic transformation of Spizellomyces punctatus, a resource for 
            studying chytrid biology and evolutionary cell biology.
  JOURNAL   Elife. 2020 May 11;9. pii: 52741. doi: 10.7554/eLife.52741.
  PUBMED    32392127
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-05-11"; volume: "9. pii: 52741. doi"; pages: " 
            10.7554/eLife.52741"
COMMENT     Sequence Label: pGI3EM30
FEATURES             Location/Qualifiers
     source          1..11631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     437..458
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(519..1544)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             complement(519..1544)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     complement(1843..1860)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1843..1859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1852..1874)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2055..2079
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2246..3049)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3050..3265)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(3468..3486)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      3527..4869
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    3859..3906
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6030..6659
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7088..8161
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8227..8421
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8765..8905
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8920..8937)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9091..9679)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9171..9190)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9774..10589)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9774..10589)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10497..10516
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10581..10600
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     misc_feature    10882..10906
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    11083..11104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11119..11124,11125..11142,11143..11149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11157..11173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11162..11184
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11181..11197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11181..11197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(11212..11399)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     11320..11344
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(11421..11631,1..497,498..500,501..503))
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNCIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELY"
     primer_bind     complement(11460..11481)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atcttgaagt tagccttgat gcagttcttc tgcttgtcgg ccgtgatata gacgtagtgg
       61 ctgttgaagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
      121 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
      181 ttgtaggtac cgtcgtcctt gaaagagatg gtgcgctcct ggacgtagcc ttcgggcatg
      241 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcaggccacg
      301 ccgtagccga aggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
      361 atgaacttca gggtcagctt gccgttggtg gcatcgccct cgccctcgcc gcggacgctg
      421 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
      481 agctcctcgc ccttgctcac catggatcct cctcctcctt cctttgccct cggacgagtg
      541 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
      601 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
      661 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
      721 agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc
      781 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
      841 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
      901 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
      961 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
     1021 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
     1081 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
     1141 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc
     1201 ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca
     1261 cggcgggaga tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga
     1321 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
     1381 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
     1441 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
     1501 aacttctcga cagacgtcgc ggtgagttca ggctttttac ccattaatta atttgtgtgt
     1561 gtgatggatg agagtggtgc gggtgaaagg tcttggtcaa gtcgcaaagt gtttgtgaaa
     1621 aagacgtagc gggggaagcg cacacacgcc cgccttttat agacgaagct tcgagcaggt
     1681 catttaacga tgcttagtag caatatacaa taaagcctaa ttgggactaa ttacggtcac
     1741 gtgaaaaact caccatgtag ccttttatgg aaatattgta cacgctacta agccactgta
     1801 atatcggcct catcgtctcc gcttggagca taaaagcttg gcactggccg tcgttttaca
     1861 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1921 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1981 cagcctgaat ggcgaatgag cttgagcttg gatcagattg tcgtttcccg ccttcagttt
     2041 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     2101 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     2161 gcatgccagg taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac
     2221 atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt
     2281 cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata
     2341 gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat
     2401 actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc
     2461 aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat
     2521 ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc
     2581 ccttgcatga caattctgct agcatcaaaa ggcctctagg ttcctttgtt acttcttctg
     2641 ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt
     2701 ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa
     2761 tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta
     2821 gcggcttaac tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta
     2881 aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat
     2941 ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa
     3001 caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc
     3061 gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt
     3121 cttcaacact acatatgcgt atatatacca atctaagtct gtgctccttc cttcgttctt
     3181 ccttctgttc ggagattacc gaatcaaaaa aatttcaagg aaaccgaaat caaaaaaaag
     3241 aataaaaaaa aaatgatgaa ttgaaaaggt ggtatggtgc actctcagta caatctgctc
     3301 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     3361 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     3421 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     3481 cctattttta taggttaatg tcatgataat aatggtttct tagtatgatc caatatcaaa
     3541 ggaaatgata gcattgaagg atgagactaa tccaattgag gagtggcagc atatagaaca
     3601 gctaaagggt agtgctgaag gaagcatacg ataccccgca tggaatggga taatatcaca
     3661 ggaggtacta gactaccttt catcctacat aaatagacgc atataagtac gcatttaagc
     3721 ataaacacgc actatgccgt tcttctcatg tatatatata tacaggcaac acgcagatat
     3781 aggtgcgacg tgaacagtga gctgtatgtg cgcagctcgc gttgcatttt cggaagcgct
     3841 cgttttcgga aacgctttga agttcctatt ccgaagttcc tattctctag aaagtatagg
     3901 aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa
     3961 aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca caaacattgc
     4021 tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc tacccatcca
     4081 cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg tttatctcta
     4141 gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga atcgaaaaca
     4201 atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga agaaaccgtt
     4261 cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca caaagtatgc
     4321 gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa aatgcacccg
     4381 cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt atggaagaga
     4441 aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca aaaagttatc
     4501 aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat gctttgttag
     4561 aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag attctttgtt
     4621 ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg
     4681 tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag cacagattct
     4741 tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc agctcagatt
     4801 ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat gaagcacaga
     4861 tgcttcgttc aggtggcact tttccttctg acgttcagtg cagccgtctt ctgaaaacga
     4921 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     4981 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     5041 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     5101 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     5161 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     5221 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     5281 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     5341 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     5401 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     5461 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     5521 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     5581 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     5641 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     5701 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     5761 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     5821 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     5881 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     5941 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     6001 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     6061 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     6121 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     6181 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     6241 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     6301 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     6361 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     6421 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     6481 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     6541 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     6601 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     6661 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     6721 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     6781 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     6841 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     6901 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     6961 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     7021 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     7081 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     7141 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     7201 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     7261 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     7321 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     7381 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     7441 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
     7501 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     7561 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     7621 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     7681 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     7741 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     7801 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     7861 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
     7921 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
     7981 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
     8041 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
     8101 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
     8161 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct
     8221 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
     8281 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
     8341 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
     8401 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
     8461 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
     8521 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
     8581 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
     8641 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     8701 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     8761 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     8821 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     8881 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8941 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9001 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9061 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9121 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9181 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9241 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9301 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9361 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9421 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9481 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     9541 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9601 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9721 gaacgaaaac tcacgttaag ggattttggt catgcaggat catgaattaa ttcttagaaa
     9781 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     9841 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     9901 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     9961 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
    10021 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
    10081 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
    10141 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
    10201 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
    10261 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
    10321 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
    10381 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
    10441 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
    10501 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
    10561 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
    10621 gacagtttta ttgttcatga tctggatcac aggcagcaac gctctgtcat cgttacaatc
    10681 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
    10741 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
    10801 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
    10861 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
    10921 acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg aattgctcta
    10981 gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    11041 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    11101 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    11161 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt cccggtagag
    11221 gtgtggtcaa taagagcgac ctcatactat acctgagaaa gcaacctgac ctacaggaaa
    11281 gagttactca agaataagaa ttttcgtttt aaaacctaag agtcacttta aaatttgtat
    11341 acacttattt tttttataac ttatttaata ataaaaatca taaatcataa gaaattcgct
    11401 tatttagaag tggcgcgcct gtacagctcg tccatgccat gtgtaatccc ggcggcggtc
    11461 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac
    11521 tgatggctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
    11581 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcaa cgttgtggcg g
//
