LOCUS       sequence-273904-        4698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High eukaryotic protein expression shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Shang H, Garretson TA, Kumar CMS, Dieter RF, Cheng XW
  TITLE     Improved pFastBac donor plasmid vectors for higher protein 
            production using the Bac-to-Bac(R) baculovirus expression vector 
            system.
  JOURNAL   J Biotechnol. 2017 Aug 10;255:37-46. doi: 
            10.1016/j.jbiotec.2017.06.397. Epub 2017 Jun 20.
  PUBMED    28645582
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biotechnol."; date: "2017-08-10"; volume: "255:37-46. doi"; pages: "
            10.1016/j.jbiotec.2017.06.397. Epub 2017 Jun 20"
COMMENT     Sequence Label: pFastBac-M2
FEATURES             Location/Qualifiers
     source          1..4698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..166
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        complement(477..568)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(535..552)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(984..989,990..1006,1007..1012)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1182..1715
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  1782..2006
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(2140..2157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2311..2899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2391..2410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3070..3861,3862..3930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3693..3712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3931..4035)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4061..4515)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4198..4219)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4409..4428
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt taaggattat
       61 ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct aatacgaaaa
      121 ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttcgga tctcctagtc
      181 gagtaacacg cccgatgtta aatatgtcct agctttattt aacgtgttta cgtcgagtca
      241 attgtacact aacgacagtg atgaaagaaa tacaaaagcg cataatattt tgaacgacgt
      301 cgaaccttta ttacaaaaca aaacacaaac gaatatcgac aaagctagat tgctgctaca
      361 agaagcttgg taccgcatgc ctcgagactg caggctctag attcgaaagc ggccgcgact
      421 agtgagctcg tcgacgtagg cctttgaatt ccgcgcgctt cggaccggga tccaatattt
      481 ataggttttt ttattacaaa actgttacga aaacagtaaa atacttattt atttgcgaga
      541 tggttatcat tttaattatc tccatgatat ctacaacatt ttggaaagtt atgttaatcc
      601 ggtgctgcta aaaaatggtg taattgaact agaagaagct gcgtactatg ctacgtagcc
      661 aaccactaga actatagcta gagtcctggg cgaacaaacg atgctcgcct tccagaaaac
      721 cgaggatgcg aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag
      781 gtcttccgat ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca
      841 gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc
      901 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
      961 aacggatgaa ggcacgaacc cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca
     1021 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1081 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tgtacagtct atgcctcggg
     1141 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     1201 atgttacgca gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca
     1261 ttcgcacatg taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt
     1321 tcggtcgtga gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc
     1381 tcgggaactt gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg
     1441 ttgttggcgc tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct
     1501 atatctatga tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca
     1561 tcaatctcct caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag
     1621 attacggtga cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga
     1681 tgcactttga tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct
     1741 tcccggccgc ggagttgttc ggtaaattgt cacaacgccg cgaatatagt ctttaccatg
     1801 cccttggcca cgcccctctt taatacgacg ggcaatttgc acttcagaaa atgaagagtt
     1861 tgctttagcc ataacaaaag tccagtatgc tttttcacag cataactgga ctgatttcag
     1921 tttacaacta ttctgtctag tttaagactt tattgtcata gtttagatct attttgttca
     1981 gtttaagact ttattgtccg cccacacccg cttacgcagg gcatccattt attactcaac
     2041 cgtaaccgat tttgccaggt tacgcggctg gtctatgcgg tgtgaaatac cgcacagatg
     2101 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2161 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2221 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2821 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2881 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2941 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3001 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3061 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3121 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3181 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3241 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3301 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3361 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3421 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3481 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3541 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3601 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3661 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3721 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3781 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3841 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3901 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3961 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4021 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     4081 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4141 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     4201 gtttggaaca agagtccact attaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     4261 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     4321 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     4381 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     4441 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     4501 cgctacaggg cgcgtcccat tcgccattca ggctgcaaat aagcgttgat attcagtcaa
     4561 ttacaaacat taataacgaa gagatgacag aaaaattttc attctgtgac agagaaaaag
     4621 tagccgaaga tgacggtttg tcacatggag ttggcaggat gtttgattaa aaacataaca
     4681 ggaagaaaaa tgccccgc
//
