LOCUS       sequence-27423-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Wollert T, Wunder C, Lippincott-Schwartz J, Hurley JH
  TITLE     Membrane scission by the ESCRT-III complex.
  JOURNAL   Nature. 2009 Mar 12. 458(7235):172-7.
  PUBMED    19234443
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Mar 12. 458(7235):172-7."
COMMENT     Sequence Label: pMBP-HIS2-Vps20_deltaC
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             628..645
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cacaacaata acaataacaa caacctcggg gatatcccaa cgaccgaaaa cctgtatttt
       61 cagggcgcca tgggatccat gggccaaaaa agtagtaaag tgcatataac caaaacagat
      121 agggcgatcc tagaagtaaa acgatctaaa gatgaaattc ataagtttac gaggaggaca
      181 gataatttga ttttggtaga gaagagtcaa ctaaaagacc taatacgaaa aaatcctgaa
      241 aattataaat ctaacatgaa ggtacgattt ctactaaaaa gaattcatta tcaggagcat
      301 ttgttgcagc aggcttcgga tcagcttatt aatttggaaa atatggtgtc aacacttgag
      361 ttcaaaatgg tagaaaagca atttattaat ggactgaaaa atggaaatga aatcttgaaa
      421 aaactaaaca aggaatttag caatgttgat gaactgatgg acgatgtaca agatcaaatt
      481 gcgtatcaaa atgaaattaa cgagacatta tccagaagct tagtcggaac aagcaattat
      541 gaagacgatc tcgataagga attggatgct ctcgaaagtg aactcaatta ggcggccgct
      601 ttcgaatcta gagcctgcag tctcgagcac caccaccacc accactgaga tccggctgct
//
