LOCUS       sequence-274421-        8239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA stop).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-F-bpA-F-L-td-sfGFP
FEATURES             Location/Qualifiers
     source          1..8239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..27
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    144..165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(180..185,186..203,204..210)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    218..234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..245
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     242..258
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     242..258
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     277..297
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        279..297
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   361..423
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        728..1107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     gap             1226..1263
                     /estimated_length=38
     intron          1390..2406
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2330..2352
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2414..2433
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(2466..2499)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2529..2753
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(2819..2852)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(2865..2898)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(2908..2910,2911..3621)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2908..3621
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             join(3652..3654,3655..4368)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3652..4365
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4426..4446)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4844..4855)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5006..5230
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5584..5618
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5705..5724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5706..5724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5731..5748)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5731..5747)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5740..5762)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5889..6344)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6026..6047)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6238..6257
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6371..6475
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6476..6544,6545..7336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6694..6713)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7507..8095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7996..8015
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
       61 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      121 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      181 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      241 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      301 cctcccccgg tattcacgac agcaggctga ataataaaaa aattagaaac tattatttaa
      361 ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc
      421 atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt
      481 tattatattt acacttacat actaataata aattcaacaa acaatttatt tatgtttatt
      541 tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacttttaaa
      601 cattctctct tttacaaaaa taaacttatt ttgtacttta aaaacagtca tgttgtatta
      661 taaaataagt aattagctta acctatacat aatagaaaca aattatactt attaaccggt
      721 catggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      781 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      841 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      901 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      961 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1021 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1081 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1141 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1201 gcagcgatgg gggcgggggg gggggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnngggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     1321 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     1381 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     1441 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1501 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     1561 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     1621 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     1681 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     1741 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     1801 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     1861 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     1921 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     1981 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg
     2041 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     2101 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     2161 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     2221 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     2281 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2341 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2401 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     2461 ctagcgaagt tcctatactt tctagagaat aggaacttcc ccggctagag ctcgctgatc
     2521 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     2581 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     2641 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     2701 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     2761 ggcggaaaga accagctggg gctcgactag agcttgcgga acccttctaa ctagataaga
     2821 agttcctata ctttctagag aataggaact tcatcgatac tagcataact tcgtatagca
     2881 tacattatac gaagttatag gcgcgccatg agcaaaggag aagaactttt cactggagtt
     2941 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga
     3001 gagggtgaag gtgatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga
     3061 aaactacctg ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt
     3121 tcccgttatc cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt
     3181 tatgtacagg aacgcactat atctttcaaa gatgacggga cctacaagac gcgtgctgaa
     3241 gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaagggtat tgattttaaa
     3301 gaagatggaa acattcttgg acacaaactc gaatacaact ttaactcaca caatgtatac
     3361 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacgtt
     3421 gaagatggtt ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     3481 cctgtccttt taccagacaa ccattacctg tcgacacaat ctgtcctttc gaaagatccc
     3541 aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat
     3601 ggcatggatg agctctacaa aggttctgct ggatctgcag caggatctgg tatgagcaaa
     3661 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     3721 gggcacaaat tttctgtccg tggagagggt gaaggtgatg ctacaaacgg aaaactcacc
     3781 cttaaattta tttgcactac tggaaaacta cctgttccgt ggccaacact tgtcactact
     3841 ctgacctatg gtgttcaatg cttttcccgt tatccggatc acatgaaacg gcatgacttt
     3901 ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac
     3961 gggacctaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc
     4021 gagttaaagg gtattgattt taaagaagat ggaaacattc ttggacacaa actcgaatac
     4081 aactttaact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct
     4141 aacttcaaaa ttcgccacaa cgttgaagat ggttccgttc aactagcaga ccattatcaa
     4201 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca
     4261 caatctgtcc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt
     4321 gtaactgctg ctgggattac acatggcatg gatgagctct acaaatgatt aattaaaacc
     4381 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4441 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4501 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4561 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4621 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4681 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4741 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4801 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4861 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4921 ccctcagacg agtcggatct ccctttgggc cgcctccccg caggatccac tagagcggcc
     4981 gcctagagct cgctatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5041 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5101 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5161 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5221 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgactagagc ttgcggaacc
     5281 cttaagcttt tttattatat attagtcacg atatctataa caagaaaata tatatataat
     5341 aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag ttaaatctta
     5401 aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta tagttcaaaa
     5461 tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg gcgactgaga
     5521 tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa tatttcaaga
     5581 atgcatgcgt caattttacg cagactatct ttctagggtt aaaaaagatt tgcgctttac
     5641 tcgacctaaa ctttaaacac gtcatagaat cttcgtttga caaaaaccac attgtgttgg
     5701 gtcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     5761 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5821 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5881 cgaatggcaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     5941 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6001 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6061 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6121 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6181 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     6241 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6301 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     6361 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6421 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6481 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6541 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6601 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6661 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6721 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6781 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6841 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6901 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6961 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7021 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7081 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7141 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7201 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7261 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7321 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7381 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7441 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7501 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7561 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7621 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7681 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7741 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7801 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7861 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7921 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7981 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8041 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8101 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8161 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8221 cgctcgccgc agccgaacg
//
