LOCUS       sequence-274591-        8961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression vector to generate a hypoxia fate-mapping 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Godet I, Shin YJ, Ju JA, Ye IC, Wang G, Gilkes DM
  TITLE     Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant 
            phenotype that promotes metastasis.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4862. doi: 10.1038/s41467-019-12412-1.
  PUBMED    31649238
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4862. doi"; pages: " 
            10.1038/s41467-019-12412-1"
COMMENT     Sequence Label: 4xHRE-MinTK-CRE-ODD
FEATURES             Location/Qualifiers
     source          1..8961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1070..1303
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1375..1496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1412..1431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1466..1485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(1661..1679)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1689..1706)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1689..1705)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1698..1720)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1847..2302
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1934..1953)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2144..2165
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2328..2432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2433..2501,2502..3293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2651..2670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3464..4052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3953..3972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4206..4223
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4340..4361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4376..4381,4382..4399,4400..4406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4414..4430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4419..4441
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4438..4454
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4438..4454
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4473..4493
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4475..4493
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4521..4747
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4748..4928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4975..5100
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5593..5826
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6011..6055
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6317..6434
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6584..6615
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     intron          6689..7261
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     7269..7288
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        7316..7334
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        7350..7368
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7350..7367
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             7403..8431
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
ORIGIN
        1 ccctaacaaa acaaagagat ggggttactc tctaaatttt atgggttatg tcattggatg
       61 ttatgggtcc ttgccacaag aacacatcat acaaaaaatc aaagaatgtt ttagaaaact
      121 tcctattaac aggcctattg attggaaagt atgtcaacga attgtgggtc ttttgggttt
      181 tgctgcccct tttacacaat gtggttatcc tgcgttgatg cctttgtatg catgtattca
      241 atctaagcag gctttcactt tctcgccaac ttacaaggcc tttctgtgta aacaatacct
      301 gaacctttac cccgttgccc ggcaacggcc aggtctgtgc caagtgtttg ctgacgcaac
      361 ccccactggc tggggcttgg tcatgggcca tcagcgcatg cgtggaacct tttcggctcc
      421 tctgccgatc catactgcgg aactcctagc cgcttgtttt gctcgcagca ggtctggagc
      481 aaacattatc gggactgata actctgttgt cctatcccgc aaatatacat cgtttccatg
      541 gctgctaggc tgtgctgcca actggatcct gcgcgggacg tcctttgttt acgtcccgtc
      601 ggcgctgaat cctgcggacg acccttctcg gggtcgcttg ggactctctc gtccccttct
      661 ccgtctgccg ttccgaccga ccacggggcg cacctctctt tacgcggact ccccgtctgt
      721 gccttctcat ctgccggacc gtgtgcactt cgcttcacct ctgcacgtcg catggagacc
      781 accgtgaacg cccaccaaat attgcccaag gtcttacata agaggactct tggactctca
      841 gcaatgtcaa cgaccgacct tgaggcatac ttcaaagact gtttgtttaa agactgggag
      901 gagttggggg aggagattag gttaaaggtc tttgtactag gaggctgtag gcataaattg
      961 gtctgcgcac cagcaccatg gcgcaatcac tagagcgggg tacctttaag accaatgact
     1021 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     1081 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     1141 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1201 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1261 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     1321 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     1381 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     1441 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     1501 gtctggctct agctatcccg cccctaactc cgcccattct ccgccccatg gctgactaat
     1561 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     1621 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     1681 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     1741 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     1801 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     1861 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1921 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1981 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     2041 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     2101 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     2161 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     2221 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     2281 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2341 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2401 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2461 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2521 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2581 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2641 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2701 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2761 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2821 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2881 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2941 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3001 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3061 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3121 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3181 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3241 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3301 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3361 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3421 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3481 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3541 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3601 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3661 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3721 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3781 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3841 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3901 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3961 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4021 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4081 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4141 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4201 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4261 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4321 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4381 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4441 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     4501 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     4561 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     4621 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     4681 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     4741 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4801 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4861 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4921 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     4981 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     5041 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     5101 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     5161 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     5221 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     5281 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     5341 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     5401 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     5461 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     5521 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     5581 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     5641 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     5701 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     5761 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     5821 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     5881 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     5941 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     6001 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     6061 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     6121 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     6181 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     6241 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     6301 atctcgacgg tatcggtttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     6361 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     6421 aaattcaaaa ttttatcgac tcagaacacg ccgtgtacgt gcttcacgtg tacgtggcga
     6481 tcgtgtacgt gctgtctcac acagcactct agactactcg tgtacgtgta cacgaagctt
     6541 cgtagcaatc ggaccgacga cgccgggctc gagatctaga ctctagaggg tatataatgg
     6601 aagctcgaat tccagcttgg cattccggta ctgttggtaa aaactaggct tttgcaaaaa
     6661 gctccggatc gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt
     6721 tttcgctatt gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag
     6781 aatgggaaga tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt
     6841 tctactctgt tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc
     6901 gttaaacttt agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga
     6961 ttgtaagtac tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac
     7021 ttcagcacag ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca
     7081 tataaattct ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat
     7141 catcctgcct ttctctttat ggttacaatg atatacactg cttgagatga ggataaaata
     7201 ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca
     7261 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgtaata
     7321 cgactcacta tagggcgaat tcaagcttga tttaggtgac actatagaat acaagctact
     7381 tgttcttttt gcaggatcca ccatgtccaa tttactgacc gtacaccaaa atttgcctgc
     7441 attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga
     7501 tcgccaggcg ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc
     7561 ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga
     7621 ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg
     7681 ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt
     7741 ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca
     7801 ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga
     7861 tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg
     7921 tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat
     7981 gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact
     8041 tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga
     8101 tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac
     8161 cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta
     8221 cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt
     8281 cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg
     8341 tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg
     8401 ggcaatggtg cgcctgctgg aagatggcga tgaattcaag ttggaattgg tagaaaaact
     8461 ttttgctgaa gacacagaag caaagaaccc attttctact caggacacag atttagactt
     8521 ggagatgtta gctccctata tcccaatgga tgatgacttc cagttacgtt ccttcgatca
     8581 gttgtcacca ttagaaagca gttccgcaag ccctgaaagc gcaagtcctc aaagcacagt
     8641 tacagtattc cagcagactc aaatacaaga acctactgct aatgccacca ctaccactgc
     8701 caccactgat gaattaaaaa cagtgacaaa agaccgtatg gaagacatta aaatattgat
     8761 tgcatctcca tctcctaccc acatacataa agaaactact tagcctagtg ccatttgttc
     8821 agtggttcgt agggctttcc cccactgttt ggctttcagt tatatggatg atgtggtatt
     8881 gggggccaag tctgtacagc atcttgagtc cctttttacc gctgttacca attttctttt
     8941 gtctttgggt atacatttaa a
//
