LOCUS       sequence-27460-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM, 
            Gray NS, Sabatini DM
  TITLE     DEPTOR is an mTOR inhibitor frequently overexpressed in multiple 
            myeloma cells and required for their survival.
  JOURNAL   Cell. 2009 May 29;137(5):873-86. doi: 10.1016/j.cell.2009.03.046. 
            Epub 2009 May 14.
  PUBMED    19446321
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2009.03.046"; journalName: "Cell"; date: 
            "2009-05-29- 29"; volume: "137"; issue: "5"; pages: "873-86"
COMMENT     Sequence Label: pRK5 FLAG DEPTOR (DEP domains)
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      700..709
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccactccca ggtccaactg cacctcggtt ctatcgattg aattcaccat ggaaatggac
       61 tacaaggatg acgatgacaa gggtggtggt ggtggtgcgt cgacggaaca gctacggctc
      121 aggctgcatg aagaaaaggt tattaaagat agacgtcatc atctcaagac ctacccaaac
      181 tgttttgtcg caaaagaact gattgactgg ctgattgaac acaaagaggc ttctgacaga
      241 gagacggcaa ttaaactcat gcagaaatta gcagaccggg gcattattca ccatgtgtgt
      301 gatgagcata aggaattcaa ggatgtcaaa ctcttctacc gctttagaaa ggatgacggc
      361 accttcccat tggataatga agtgaaggcc tttatgagag gacagaggct atatgaaaag
      421 ctgatgagcc ctgaaaacac actcctgcag cccagggagg aggaaggggt caagtatgag
      481 cgcaccttca tggcatctga attcctggac tggctggttc aggaaggtga ggccaccacg
      541 aggaaagagg cagagcagct ttgccaccgg cttatggagc atggcatcat ccagcatgtg
      601 tccagcaagc acccatttgt ggacagcaat cttctctacc agttcagaat gaacttccgg
      661 cggtgagcgg ccgctaagta agtaaggatc cccagcttgg ccgccatggc ccaacttgtt
      721 tattgcagct tatatggtt
//
