LOCUS       sequence-274615-       11151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides episomal replication of the destination vector in primate 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11151)
  TITLE     Minoru Ko Lab plasmids
REFERENCE   2  (bases 1 to 11151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDEST-EB-Hyg-R4R3
FEATURES             Location/Qualifiers
     source          1..11151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        308..453
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             496..1533
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(1531..1550)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1575..1623
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2039..2055
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2039..2055
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2075..2198
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2546..2851)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2648..2667)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(3171..3854)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     3769..3788
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3855..3957)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(4103..4226)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4234..4250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(6748..8673)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGGXGGAGGAGGXGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGXGGXGXGGGAGAGGGAGGAGAGGGAGGXGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGXGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(9108..9126)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9194..9298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9299..9367,9368..10159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9517..9536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10330..10918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10819..10838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
       61 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
      121 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agsmwyksrt
      181 ygsstccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccctgct tcatccccgt
      241 ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag
      301 ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat
      361 gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
      421 gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc cgcagatccc
      481 ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
      541 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
      601 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
      661 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
      721 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
      781 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
      841 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
      901 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
      961 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
     1021 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     1081 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
     1141 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     1201 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
     1261 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
     1321 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
     1381 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
     1441 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
     1501 cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     1561 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
     1621 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     1681 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     1741 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc actggccccg
     1801 tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg ttattatttt
     1861 gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga
     1921 tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc
     1981 aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag ctgctagtca
     2041 ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttgaacga
     2101 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     2161 acataatact gtaaaacaca acatatccag tcactatggt cgaccgacag ccttccaaat
     2221 gttcttctca aacggaatcg tcgtatccag cctactcgct attgtcctca atgccgtatt
     2281 aaatcataaa aagaaataag aaaaagaggt gcgagcctct tttttgtgtg acaaaataaa
     2341 aacatctacc tattcatata cgctagtgtc atagtcctga aaatcatctg catcaagaac
     2401 aatttcacaa ctcttatact tttctcttac aagtcgttcg gcttcatctg gattttcagc
     2461 ctctatactt actaaacgtg ataaagtttc tgtaatttct actgtatcga cctgcagact
     2521 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
     2581 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca
     2641 cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt
     2701 aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc
     2761 gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct
     2821 ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa
     2881 gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc
     2941 cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag
     3001 atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa
     3061 tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat cagtatatat
     3121 tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc
     3181 ggatccacgc ggcgtttacg ccccccctgc cactcatcgc agtactgttg taattcatta
     3241 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     3301 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     3361 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     3421 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     3481 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     3541 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     3601 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     3661 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     3721 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     3781 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     3841 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     3901 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg
     3961 tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt
     4021 atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt
     4081 gatgctgcca acttacggcc gccatagtga ctggatatgt tgtgttttac agtattatgt
     4141 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     4201 tcgttcaact ttattataca tagttgataa ttcactggcc gtcgttttac ctcgactcta
     4261 gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc
     4321 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt
     4381 ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc
     4441 ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg
     4501 cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac
     4561 accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca
     4621 acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca
     4681 atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt
     4741 agtatatact atccagacta accctaattc aatagcatat gttacccaac gggaagcata
     4801 tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg
     4861 agctgtcacg gttttattta catggggtca ggattccacg agggtagtga accattttag
     4921 tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct
     4981 gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt
     5041 gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg
     5101 ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca
     5161 ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg
     5221 tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca
     5281 cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag
     5341 acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac
     5401 agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca
     5461 atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc
     5521 acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac
     5581 ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa
     5641 tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc
     5701 tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg
     5761 tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat
     5821 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta
     5881 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     5941 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     6001 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     6061 tcctcatgca tatacggata gcatatacta ccccaanaaa tatctggata gcatatgcta
     6121 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     6181 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     6241 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     6301 tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca gcatatgata
     6361 cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca agggggcgtg
     6421 aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga atttaaggag
     6481 gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc
     6541 aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta tgcccaattg
     6601 ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac accaacagca
     6661 cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc ttttaatacg
     6721 attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct catctccatc
     6781 acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt ccaccatagg
     6841 tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca ccctgatatt
     6901 gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca aaacctcagc
     6961 aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc ttagcgggcc
     7021 aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg gtgtaagacg
     7081 acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg tcttactacc
     7141 tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt ctacgtgact
     7201 cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt tggaacctcc
     7261 ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat caccctgacc
     7321 ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct ctatcgctcc
     7381 cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa aataatcggc
     7441 ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg gtggagaccc
     7501 ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc cacgacctct
     7561 ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac cccggcggcc
     7621 tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc cactgcctcc
     7681 tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc
     7741 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     7801 ccctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc
     7861 tgctcctgcc cctcctsccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     7921 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
     7981 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc
     8041 tcctgcccct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctmcccc
     8101 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
     8161 tsctcctscc cctcctsccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     8221 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc
     8281 tcctgcccct cctcctgctc ctgcccctcc tscycctcct gcccctcctg cccctcctsc
     8341 ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc
     8401 tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct
     8461 ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc
     8521 gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg
     8581 aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt
     8641 tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac
     8701 atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag
     8761 cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc
     8821 catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct
     8881 gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct
     8941 ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac
     9001 cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc
     9061 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
     9121 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     9181 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9241 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     9301 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     9361 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     9421 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     9481 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     9541 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     9601 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     9661 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     9721 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9781 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9841 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9901 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9961 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    10021 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    10081 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    10141 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    10201 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    10261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    10321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    10381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    10441 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    10501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    10561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    10621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg
    10981 atggtcgtca tctacctgcc tggacwsyry srmskgttgg tttgcgcatt cacagttctc
    11041 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccct
    11101 gcttcnatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga a
//
