LOCUS       sequence-274957-        3831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Degradation tag variant at C-terminal of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Teh MY, Ooi KH, Danny Teo SX, Bin Mansoor ME, Shaun Lim WZ, Tan MH
  TITLE     An Expanded Synthetic Biology Toolkit for Gene Expression Control in
            Acetobacteraceae.
  JOURNAL   ACS Synth Biol. 2019 Apr 19;8(4):708-723. doi: 
            10.1021/acssynbio.8b00168. Epub 2019 Mar 22.
  PUBMED    30865830
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00168"; journalName: "ACS Synth Biol"; date: 
            "2019-04-19- 19"; volume: "8"; issue: "4"; pages: "708-723"
COMMENT     Sequence Label: MYMH 553
FEATURES             Location/Qualifiers
     source          1..3831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(197..283)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     298..320
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        328..362
                     /label=BBa_J23104
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.72"
     RBS             370..381
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             388..1095
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(533..551)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     801..820
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1161..1232
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1248..1275
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1282..1302
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1307..1401
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1449..1551
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1552..2211
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1618..1637)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     oriT            2360..2468
                     /note="incP origin of transfer"
     CDS             complement(2479..3141)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      join(3142..3831,1..82)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac gggccgagcg
       61 aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg ctggcaaggg
      121 agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggggg gcgcgcccag
      181 ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
      241 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctt taattaaagc
      301 ggataacaat ttcacacagg agaattcttg acagctagct cagtcctagg tattgtgcta
      361 gctctagaga aagaggagaa atactagatg gtgagcaagg gcgaggagga taacatggcc
      421 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      481 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      541 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      601 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      661 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      721 gtgacccagg actcctcctt gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      781 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      841 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
      901 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      961 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     1021 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     1081 gacgagctgt acaagggatc cgctgctaac gacgaaaact acaactacgc tgacgcttct
     1141 taacctgcag ggccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1201 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1261 gtgggccttt ctgcgtttat atactagtag cggccgctgc agcttggact cctgttgata
     1321 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     1381 gcgtttttta ttggtgagaa tccaggggtc cccaataatt acgatttaaa ttggcgaaaa
     1441 tgagacgttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     1501 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
     1561 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1621 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1681 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1741 gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg tgagctggtg
     1801 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     1861 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     1921 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     1981 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     2041 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     2101 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     2161 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta atttgacttt
     2221 tgtccttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
     2281 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
     2341 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
     2401 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
     2461 gctggccgta ggccggccct accggcgcgg cagcgttacc cgtgtcggcg gctccaacgg
     2521 ctcgccatcg tccagaaaac acggctcatc gggcatcggc aggcgctgct gcccgcgccg
     2581 ttcccattcc tccgtttcgg tcaaggctgg caggtctggt tccatgcccg gaatgccggg
     2641 ctggctgggc ggctcctcgc cggggccggt cggtagttgc tgctcgcccg gatacagggt
     2701 cgggatgcgg cgcaggtcgc catgccccaa cagcgattcg tcctggtcgt cgtgatcaac
     2761 caccacggcg gcactgaaca ccgacaggcg caactggtcg cggggctggc cccacgccac
     2821 gcggtcattg accacgtagg ccgacacggt gccggggccg ttgagcttca cgacggagat
     2881 ccagcgctcg gccaccaagt ccttgactgc gtattggacc gtccgcaaag aacgtccgat
     2941 gagcttggaa agtgtcttct ggctgaccac cacggcgttc tggtggccca tctgcgccac
     3001 gaggtgatgc agcagcattg ccgccgtggg tttcctcgca ataagcccgg cccacgcctc
     3061 atgcgctttg cgttccgttt gcacccagtg accgggcttg ttcttggctt gaatgccgat
     3121 ttctctggac tgcgtggcca tgcttatctc catgcggtag gggtgccgca cggttgcggc
     3181 accatgcgca atcagctgca acttttcggc agcgcgacaa caattatgcg ttgcgtaaaa
     3241 gtggcagtca attacagatt ttctttaacc tacgcaatga gctattgcgg ggggtgccgc
     3301 aatgagctgt tgcgtacccc ccttttttaa gttgttgatt tttaagtctt tcgcatttcg
     3361 ccctatatct agttctttgg tgcccaaaga agggcacccc tgcggggttc ccccacgcct
     3421 tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg ggcttgttga tcgactgcgc
     3481 ggccttcggc cttgcccaag gtggcgctgc ccccttggaa cccccgcact cgccgccgtg
     3541 aggctcgggg ggcaggcggg cgggcttcgc ccttcgactg cccccactcg cataggcttg
     3601 ggtcgttcca ggcgcgtcaa ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg
     3661 ccttccactt ggtgtccaac cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc
     3721 cccagagaaa attaaaaaaa ttgatggggc aaggccgcag gccgcgcagt tggagccggt
     3781 gggtatgtgg tcgaaggctg ggtagccggt gggcaatccc tgtggtcaag c
//
