LOCUS       sequence-275022-        2777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP for N-term fusion with a CTAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CDS12_mVenus
FEATURES             Location/Qualifiers
     source          1..2777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..67
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(71..114)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             join(131..133,134..136,137..847)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(176..197)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(437..456)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     784..805
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      867..924
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(948..965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1119..1707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1199..1218)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1890..1984)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2005..2664)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2579..2598
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
       61 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat
      121 tcggtctcaa atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      181 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      241 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc
      301 ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga
      361 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      421 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      481 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      541 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa
      601 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
      661 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      721 cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga
      781 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
      841 gtacaaggct tcgcgagacc ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc
      901 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
      961 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1021 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1081 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
     1141 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1201 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1261 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1321 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1381 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1441 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1501 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1561 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1621 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1681 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1741 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1801 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1861 taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     1921 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     1981 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     2041 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     2101 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     2161 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     2221 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     2281 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     2341 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     2401 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     2461 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     2521 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     2581 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2641 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     2701 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     2761 cccgatcaac tcgagtg
//
