LOCUS       sequence-275125-        2790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP with ER retention signal for C-term fusion with CDS12 with ER 
            targeting signal peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CTAG_mVenus-HDEL
FEATURES             Location/Qualifiers
     source          1..2790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..145
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     385..406
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     terminator      468..511
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(515..533)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             695..1354
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(761..780)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1375..1469
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1652..2240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2141..2160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2394..2411
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2435..2492)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(join(2525..2790,1..445,446..448))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2567..2588)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
       61 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      121 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      181 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc
      241 actgcaggcc gtagcccagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      301 tggtgcagat cagcttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
      361 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      421 cggtgaacag ctcctcgccc ttgctcaccg aatgagaccg aattccagaa atcatcctta
      481 gcgaaagcta aggatttttt ttatctgaaa ttctgcctcg tgatacgcct atttttatag
      541 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcactcgagt tgatcgggca
      601 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
      661 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
      721 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
      781 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
      841 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
      901 tcatccggaa tttcgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
      961 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1021 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1081 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1141 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1201 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1261 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     1321 acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc
     1381 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
     1441 gccgccgggc gttttttatt ggtgagaatc caagcctcga gctgtcagac caagtttact
     1501 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1561 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1621 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1681 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1741 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1801 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1861 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1921 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1981 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2041 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2101 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2161 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2221 gggggcggag cctgtggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2281 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2341 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2401 cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc
     2461 agggtggtga caccttgccc ttttttgccg gactgcaggg tctcgaagct taaagctcat
     2521 catgcttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     2581 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg tagctcaggt
     2641 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     2701 cggcgagctg cacgccgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc
     2761 cgttcttctg cttgtcggcg gtgatataga
//
