LOCUS       sequence-27515-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA245
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(510..605)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(645..662)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(645..661)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(654..676)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggnagtcttc tcctttctca ttctctgtct gaaacattca attgattatc gataaaatta
       61 attaagagag aatacaatga gaaaacggaa aatcgagcag gattttgggg aaagtcgggg
      121 aatttttgct atttaagctt tctttaacaa agaaactgca tttttccatg agattaccat
      181 gaaatttgcg ttaaatgggt gaatttataa catttacggc tagaaattaa ttttaaaaaa
      241 gagaataaac gaaaaattat tcgagaaaac gtctttgaag cagaaatgaa atgaaaaaac
      301 aaccgccagc gctgacaaaa atgatcaaac aaacggagta cggtatacgc ggtaagaccc
      361 aaatgaattt taattcggtg cgcctttaaa aaatgtattt ttttaatttc tttatccgtg
      421 ggcccaaatg tatttttttc ttgtggtttg atgtaaaaat tgggttatat tatttgtttt
      481 atggtttaat tttttaaaaa ctgatatttc aacttttcta tacaaagttg gcattataaa
      541 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      601 tatttggagc tccatggtag cgttaactcg ggcccgagct taagactggc cgtcgtttta
      661 caacgtcgtg actgggaaaa catccatgct agcgttaacg cgagagtagg gaactgcc
//
