LOCUS       sequence-27521-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J
  TITLE     MosSCI and gateway compatible plasmid toolkit for constitutive and 
            inducible expression of transgenes in the C. elegans germline.
  JOURNAL   PLoS One. 2011;6(5):e20082. Epub 2011 May 26.
  PUBMED    21637852
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5)"; pages: "e20082. Epub 2011 May 26"
COMMENT     Sequence Label: pJA256
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..128
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(133..456)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(505..525)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcgcggccgc gttaacgcta ccatggagct ccaaataatg attttatttt gactgatagt
       61 gacctgttcg ttgcaacaaa ttgataagca atgctttttt ataatgccaa ctttgtataa
      121 taaagttgct gagacttttt tcttggcggc acaataaagt ttcctctttt tgttgggtca
      181 ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt tctcaacctt tcgggtttaa
      241 agcacttcaa aatggaattg aagagagatt gtgaaagtac atcagacaga atcacatttt
      301 gatagcattc acttcactca gatgcaaact tttcagtgac tataaaaagt cataaaataa
      361 taacattaaa aaagcaaata aattagcgag agaatttttt gacaaaaaga aagaagagtg
      421 atagagaaga agggaatgct tgaaaggatt ttgcatttat ctcatttgta tagttcgtcc
      481 atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt
      541 tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg
      601 acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca
      661 tcttcaatgt tgtgtctaat tttgaagttc tgaaaattta aatcagatta gttagttagt
      721 aaaatcatgt ttaaacttac aactttgatt ccattctttt gtttgtctgc catgatg
//
