LOCUS       sequence-275231-        6812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses split GFP fragment (GFP1-10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Kondo S, Takahashi T, Yamagata N, Imanishi Y, Katow H, Hiramatsu S, 
            Lynn K, Abe A, Kumaraswamy A, Tanimoto H
  TITLE     Neurochemical Organization of the Drosophila Brain Visualized by 
            Endogenously Tagged Neurotransmitter Receptors.
  JOURNAL   Cell Rep. 2020 Jan 7;30(1):284-297.e5. doi: 
            10.1016/j.celrep.2019.12.018.
  PUBMED    31914394
REFERENCE   2  (bases 1 to 6812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.12"; journalName: "Cell Rep"; date: 
            "2020-01-7- 7"; volume: "30"; issue: "1"; pages: "284-297.e5"
COMMENT     Sequence Label: pBFv-UAS3-GFP1-10
FEATURES             Location/Qualifiers
     source          1..6812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    39..133
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    165..259
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        288..526
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     473..494
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(1777..1797)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1777..1795)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1816..1832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1816..1832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1829..1851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1840..1856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1864..1870,1871..1888,1889..1894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1909..1930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2047..2064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2218..2806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2298..2317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2977..3768,3769..3837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3600..3619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3838..3942)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3968..4423)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4105..4126)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4317..4336
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4550..4572
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4564..4581
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4565..4581
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4591..4610
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4591..4609
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4817..4886
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             complement(join(5086..5203,5280..5373,5465..6071,6126..6259,
                     6320..6480,6554..6579))
                     /codon_start=1
                     /product="tryptophan oxygenase"
                     /label=vermilion
                     /note="selectable marker, required to synthesize the brown 
                     eye pigment in Drosophila"
                     /translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
                     EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
                     ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
                     SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
                     QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
                     YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
                     SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
ORIGIN
        1 ttccctgcag ggccaaccaa caaccttaag ctgcaggtcg gagtactgtc ctccgagcgg
       61 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
      121 actgtcctcc gagcggagac tctagcgagc acgcgtctgc aggtcggagt actgtcctcc
      181 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
      241 cggagtactg tcctccgagc ggagactcta gcgagccata tggagcgccg gagtataaat
      301 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
      361 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
      421 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
      481 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata gggaattggg
      541 gcgcgcccaa aatgtccaaa ggagaagagc tgttcactgg agtggtacca atacttgtgg
      601 agttggacgg agatgtgaac ggacacaaat tttcagtccg cggggagggg gaaggggatg
      661 ctactattgg caagctgacg ctcaaattca tctgtaccac cggaaaactc cctgtaccct
      721 ggcccacact ggtgacaact ctgacttacg gcgtgcaatg ttttagccga tacccagacc
      781 acatgaagag gcacgacttt ttcaaaagcg caatgcctga aggatacgta caggaaagga
      841 ccatttcttt taaagacgac gggaagtaca aaacccgggc agtggtgaag tttgagggcg
      901 ataccctcgt caataggatc gaattgaagg gaactgactt caaagaagat ggcaacatcc
      961 tgggtcacaa gcttgagtat aactttaact cccacaacgt gtatattaca gccgacaaac
     1021 agaagaatgg aattaaggct aacttcactg tcagacacaa tgtcgaagat ggctccgtgc
     1081 agctcgccga tcactatcaa cagaatactc ctatcgggga cggcccagtc ctgctgcccg
     1141 acaaccacta cctgagtacc cagactgttc tgagcaaaga tccgaacgag aagtaagcgg
     1201 ccgcgcgtca cgccacttca acgctcgatg ggagcgtcat tggtgggcgg ggtaaccgtc
     1261 gaaatcagtg tttacgcttc caatcgcaac aaaaaattca ctgcaacact gaaaagcata
     1321 cgaaaacgat gaagattgta cgagaaacca taaagtattt tatccacaaa gacacgtata
     1381 gcagaaaagc caagttaact cggcgataag ttgtgtacac aagaataaaa tcggccagat
     1441 tcagtgttgt cagaaataag aaaaccccac tatgtttttc tttgcctttt ctttctccca
     1501 gcgatcattc atttcgtggt gaaagaacgg ggtcattgca cggagtttcg actgcgggaa
     1561 agcagagctg ccgttcactt cgtctataat tagcgctttc tattttcccc gattcgggcc
     1621 gctgctgcgc ttttccgcct gctgtttgtg gcaagtgtag cagcaggctg tgcacgcagt
     1681 gtgtagggat aacagggtaa tgacgtcgca tgcacttggc tttccaccgt tggtatcgat
     1741 tctctgggac gatgagtcga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     1801 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1861 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1921 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1981 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2041 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2101 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2161 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2221 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2281 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2341 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2401 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2461 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2521 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2581 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2641 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2701 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2761 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2821 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2881 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2941 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3001 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3061 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3121 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3181 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3241 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3301 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3361 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3421 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3481 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3541 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3601 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3661 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3721 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3781 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3841 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3901 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3961 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4021 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4081 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4141 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4201 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4261 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     4321 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4381 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     4441 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     4501 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     4561 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     4621 acctcgacat gcccgccgtg accgtcgaga acccgctgac gctgccccgc gtatccgcac
     4681 ccgccgacgc cgtcgcacgt cccgtgctca ccgtgaccac cgcgcccagc ggtttcgagg
     4741 gcgagggctt cccggtgcgc cgcgcgttcg ccgggatcaa ctaccgccac ctcgacccgt
     4801 tcatcatgat ggaccagatg ggtgaggtgg agtacgcgcc cggggagccc aagggcacgc
     4861 cctggcaccc gcaccgcggc ttcgagaccg tgacctacat cgtcgacaag cttggattta
     4921 ttttgttatg ttatatgtat tatatgtcag acataaagaa aaggaacaca tcaaatgtga
     4981 taacaaagac taaacaagta attttattac accaaaacga caaaacagta ggcagaacaa
     5041 acaacgcata gccaaacatt gacgaattgg ataccctgcc gattgtcaga cacttttgtt
     5101 gatcagtttc ttgcgaatgg tctcgtccag cggtggaatc gcctcgcggg gaatcagaaa
     5161 agtggacaga ttgaacagat ccagaaacac cttgtaccga tcactgaaac caaaaaaaaa
     5221 caaagggaga acagtttgag ttcattgatc cccgatataa tcacatctgc gatgatcacc
     5281 tgagagtgga gcgcagatat tgatatccag acgagccacc agtgcccaac tgttgggatc
     5341 caatcatgcg ttgcaccatg atcacgtgat tgtctgcggc gggaatagaa agtatttggt
     5401 taggaaaacc agtcttaaac ataagatata tttataaaag agtatcaaag aatgcaatac
     5461 ttacatctcc acttggttat taacgagtcg atgtccatga gcagggtgag caactggtgt
     5521 ggttggctga acctgggttc atccctatag aaggtgatca tgatggctcc ctgaagggca
     5581 cgatggctaa accggcgatc cccacgacgc accagtgcat cgtgcactgc cggatcaaag
     5641 atggagcgat acacctcgcg tcgcttctca atgtccatga ggcggtagtt tttcgccttc
     5701 tccacgggct cctccatggc gctctgtacc tgcgcctcca ggaatcgatc gacgctctcc
     5761 tgaaacttgg cccagaagtt gaagccactc tcctccagtc cgggcgtcct ctccagccat
     5821 cgctgcacta gctccagtag cgagggatct ttctccgagt tgcgaatcga gttccgcgcc
     5881 tcctcgtcgc taaagacatc cgagtacttc tggttgtatc tcacccgctg ctctgtcaga
     5941 actcccagct tgttctcgat caaacggaac tgcagcgact gaaaaccaga tgcgggtgcc
     6001 aggtacttgc ggaagtccat gaagtctagc ggggtcatgg tctccagaat gggcacttgg
     6061 tccaccagga gctgtacaaa ggaagttata aacggatttt ggtaagagat tcagaaagca
     6121 ctcactttta gaatcagaac cactcggttc agtcgcttga caatctccag cgtcttggtt
     6181 tcatcgatga cctctgcatc caacatgtct cgtatggagt cgaactcaaa gatgatctgc
     6241 ttgaaccaaa gctcgtaggc tgtggcgaag gtacttaaat gccattgagt gttgtcatca
     6301 aagttgtaaa cctactcacc ctggtgcgtg atgatgaaca gatgctcatc gtgcacgggt
     6361 cgcttgtcct cctcggacag catacactgg gcatccagca gtttgtccag catcagatac
     6421 tctccataga ttttgcccac ttccgtggtt aatggcaccg ccgaatcatc gtgatcgttt
     6481 ctgtatgggt ttgaattgaa tcgcagaact gaagatcgat tggcattcct ggacagcacg
     6541 tgctggtgct cacccgtttc ctgcataggg acagctcatg gtgcacagct cagatcagat
     6601 cgtgactcct cgagcggcgg atgctggcga actgatctcc gccagcggac cggagatgag
     6661 accccagcga accgataaca gagcgagaga gctccagttc cgactgattg cacagtcggt
     6721 gatctgggcg atgggcactg ccagataggc tgggaattat caatcacttg aggtgaaagt
     6781 gcggcgcaca caaatccaag cttgatatcg aa
//
