LOCUS       sequence-275303-        2757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FP for C-term fusion with CDS12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Sauret-Gueto S, Frangedakis E, Silvestri L, Rebmann M, Tomaselli M, 
            Markel K, Delmans M, West A, Patron NJ, Haseloff J
  TITLE     Systematic tools for reprogramming plant gene expression in a simple
            model, Marchantia polymorpha.
  JOURNAL   ACS Synth Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511.
  PUBMED    32163700
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Mar 12. doi: 10.1021/acssynbio.9b00511."
COMMENT     Sequence Label: CTAG_mScarlet-I
FEATURES             Location/Qualifiers
     source          1..2757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(62..81)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(753..847)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(868..1527)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1442..1461
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1689..1707
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1711..1754)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             complement(1777..2469)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(2052..2071)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2321..2339
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      2487..2544
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2568..2585)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
       61 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      721 gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg
      781 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
      841 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
      901 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
      961 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     1021 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     1081 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     1141 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     1201 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     1261 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     1321 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     1381 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     1441 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     1501 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     1561 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     1621 gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     1681 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     1741 ctaaggatga tttctggaat tcggtctcaa agcttacttg tacagctcgt ccatgccgcc
     1801 ggtggagtgg cggccctcgg agcgttcgta ctgttccacc acggtgtagt cctcgttgtg
     1861 ggaggtgatg tccaacttgc ggtcgacgtt gtaggcgccg ggcatctgca cgggcttctt
     1921 ggccttgtag gtggtcttga agtccgccag gtagcggccg ccgtccttca ggcgcagggc
     1981 catcttaatg tcgcccttca gcacgccgtc ctcggggtac aaccgctcgg tggacgcttc
     2041 ccagcccatt gtcttcttct gcattacggg gccgtcagga gggaagttgg tgccgcggag
     2101 cttcaccttg tagatcaggg tgccgtcctc cagggaggtg tcctgggtca cggtcacggc
     2161 gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggactgctt
     2221 atagtagtcg gggatgtcgg cggggtgctt gatgaaggcc ctggagccgt acatgaactg
     2281 aggggacagg atgtcccagg agaagggcag ggggccaccc ttggtcacct tcagcttggc
     2341 ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc
     2401 gttcatggag ccctccatgt gcaccttgaa ccgcatgaac tccttgatca ctgcctcgcc
     2461 cttgctcacc gaacgagacc ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc
     2521 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
     2581 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2641 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2701 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccc
//
