LOCUS       sequence-275313-        9702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of the cell cycle marker PIP-FUCCI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Grant GD, Kedziora KM, Limas JC, Cook JG, Purvis JE
  TITLE     Accurate delineation of cell cycle phase transitions in living cells
            with PIP-FUCCI.
  JOURNAL   Cell Cycle. 2018;17(21-22):2496-2516. doi: 
            10.1080/15384101.2018.1547001.
  PUBMED    30421640
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2018"; volume: "17(21-22):2496-2516. doi"; pages: " 
            10.1080/15384101.2018.1547001"
COMMENT     Sequence Label: pLenti-CMV-Blast-PIP-FUCCI
FEATURES             Location/Qualifiers
     source          1..9702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    45..633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(98..118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(516..527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(684..704)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        689..1018
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      869..1004
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1066..1113
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1132..1530
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1617..1850
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1922..2043
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1959..1978)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2013..2032
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2083..2218
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2238..2257)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2239..2257)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2267..2284)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2267..2283)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2276..2298)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2425..2880
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2512..2531)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2722..2743
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2906..3010
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3011..3079,3080..3871)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3229..3248)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4042..4630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4531..4550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4784..4801
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4918..4939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4954..4959,4960..4977,4978..4984)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4992..5008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4997..5019
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5016..5032
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5016..5032
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5051..5071
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5053..5071
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5099..5325
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5326..5506
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5553..5678
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6171..6404
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6589..6633
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6900..7016
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        7039..7342
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7343..7546
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7497..7517
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7543..7567
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7651..7675
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7747..7767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7777..7803
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7807..7833
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7840..7842,7843..7845,7846..8553)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(7885..7906)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8146..8165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8493..8514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8569..8625
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8632..9339
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8777..8795)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9045..9064
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             9346..9675
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     protein_bind    complement(join(9692..9702,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt
       61 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
      121 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
      181 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
      241 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
      301 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
      361 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
      421 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
      481 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
      541 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
      601 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctgcagata tccggttagt
      661 aatgagtttg gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      721 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      781 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      841 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      901 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
      961 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     1021 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     1081 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     1141 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     1201 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     1261 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     1321 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     1381 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     1441 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     1501 ctgccctctg gttatgtgtg ggagggctaa gcacaattcg agctcggtac ctttaagacc
     1561 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     1621 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     1681 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1741 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1801 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     1861 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     1921 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1981 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     2041 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     2101 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2161 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2221 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     2281 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2341 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2401 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2461 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2521 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2581 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2641 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     2701 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     2761 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     2821 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     2881 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     2941 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3001 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3061 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3121 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3181 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3241 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3301 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3361 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     3421 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     3481 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     3541 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     3601 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     3661 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     3721 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     3781 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     3841 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     3901 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     3961 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4021 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4081 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4141 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4201 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4261 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4321 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4381 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     4441 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4501 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     4561 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     4621 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4681 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     4741 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4801 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     4861 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     4921 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     4981 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     5041 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     5101 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     5161 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     5221 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     5281 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     5341 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     5401 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     5461 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     5521 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     5581 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     5641 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     5701 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     5761 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     5821 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     5881 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     5941 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     6001 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     6061 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     6121 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     6181 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     6241 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     6301 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     6361 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     6421 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     6481 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     6541 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     6601 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     6661 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     6721 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     6781 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     6841 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     6901 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     6961 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     7021 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     7081 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     7141 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     7201 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     7261 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     7321 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     7381 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     7441 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     7501 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     7561 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     7621 gtccagtgtg gtggaattct gcagatatca acaagtttgt acaaaaaagc aggcttcgaa
     7681 ttcatggagc agcgccgcgt caccgacttc ttcgcgcgcc gccgccccgg gccccggaca
     7741 cgtggcccaa aaaagaagag aaaggtagga gctcaatacc catacgatgt tcctgactat
     7801 gcgggctatc cctatgacgt cccggactat gcaactagta tggtgagcaa gggcgaggag
     7861 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     7921 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
     7981 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
     8041 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     8101 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     8161 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     8221 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     8281 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     8341 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
     8401 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc
     8461 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     8521 gccgggatca ctctcggcat ggacgagctg tacctcgagg gaagcggagc tactaacttc
     8581 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctcaatt gatggtgagc
     8641 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     8701 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     8761 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     8821 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     8881 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     8941 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     9001 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     9061 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     9121 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     9181 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     9241 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     9301 ggccgccact ccaccggcgg catggacgag ctgtacaagg tcgagatgaa tcccagtatg
     9361 aagcagaaac aagaagaaat caaagagaat ataaagaata gttctgtccc aagaagaact
     9421 ctgaagatga ttcagccttc tgcatctgga tctcttgttg gaagagaaaa tgagctgtcc
     9481 gcaggcttgt ccaaaaggaa acatcggaat gaccacttaa catctacaac ttccagccct
     9541 ggggttattg tcccagaatc tagtgaaaat aaaaatcttg gaggagtcac ccaggagtca
     9601 tttgatctta tgattaaaga aaatccatcc tctcagtatt ggaaggaagt ggcagaaaaa
     9661 cggagaaagg cgctgtaaca attgggatcc tacccagctt tc
//
