LOCUS       sequence-275348-        8156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8156)
  TITLE     Structural Genomics Consortium Clones
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDB1
FEATURES             Location/Qualifiers
     source          1..8156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..225
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(292..825)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(995..1000,1001..1017,1018..1023))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        1375..1466
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     1391..1408
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1534..1551
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1561..1578
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     polyA_signal    5136..5270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5173..5192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5227..5246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5299..5464
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5648..6102
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5735..5754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5944..5965
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6128..6232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6233..6301,6302..7093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6451..6470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7264..7852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7753..7772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8006..8023
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt
       61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat
      121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc
      181 gtattaaaga ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa
      241 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
      301 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
      361 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
      421 gttggcctca tgcttgagca gattgatgag cgcggtggca atgccctgcc tccggtgctc
      481 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
      541 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
      601 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
      661 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
      721 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
      781 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
      841 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
      901 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      961 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
     1021 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
     1081 cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt cggtctccac
     1141 gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt gcacggatct
     1201 gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac
     1261 cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt tgttcgccca
     1321 ggactctagc tatagttcta gtggttggct acgtatactc cggaatatta atagatcatg
     1381 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     1441 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
     1501 gcggatctcg gtccgaaaac catgggcagc agccatcatc atcatcatca cagcagcggc
     1561 ctggttccgc gtggtagtat gtcgtacaac tacgtggtaa cggcccagaa gcccaccgcc
     1621 gtgaacggct gcgtgaccgg acactttact tcggccgaag acttaaacct gttgattgcc
     1681 aaaaacacga gattagagat ctatgtggtc accgccgagg ggcttcggcc cgtcaaagag
     1741 gtgggcatgt atgggaagat tgcggtcatg gagcttttca ggcccaaggg ggagagcaag
     1801 gacctgctgt ttatcttgac agcgaagtac aatgcctgca tcctggagta taaacagagt
     1861 ggcgagagca ttgacatcat tacgcgagcc catggcaatg tccaggaccg cattggccgc
     1921 ccctcagaga ccggcattat tggcatcatt gaccctgagt gccggatgat tggcctgcgt
     1981 ctctatgatg gccttttcaa ggttattcca ctagatcgcg ataataaaga actcaaggcc
     2041 ttcaacatcc gcctggagga gctgcatgtc attgatgtca agttcctata tggttgccaa
     2101 gcacctacta tttgctttgt ctaccaggac cctcaggggc ggcacgtaaa aacctatgag
     2161 gtgtctctcc gagaaaagga attcaataag ggcccttgga aacaggaaaa tgtcgaagct
     2221 gaagcttcca tggtgatcgc agtcccagag ccctttgggg gggccatcat cattggacag
     2281 gagtcaatca cctatcacaa tggtgacaaa tacctggcta ttgcccctcc tatcatcaag
     2341 caaagcacga ttgtgtgcca caatcgagtg gaccctaatg gctcaagata cctgctggga
     2401 gacatggaag gccggctctt catgctgctt ttggagaagg aggaacagat ggatggcacc
     2461 gtcactctca aggatctccg tgtagaactc cttggagaga cctctattgc tgagtgcttg
     2521 acataccttg ataatggtgt tgtgtttgtc gggtctcgcc tgggtgactc ccagcttgtg
     2581 aagctcaacg ttgacagtaa tgaacaaggc tcctatgtag tggccatgga aacctttacc
     2641 aacttaggac ccattgtcga tatgtgcgtg gtggacctgg agaggcaggg gcaggggcag
     2701 ctggtcactt gctctggggc tttcaaggaa ggttctttgc ggatcatccg gaatggaatt
     2761 ggaatccacg agcatgccag cattgactta ccaggcatca aaggattatg gccactgcgg
     2821 tctgacccta atcgtgagac tgatgacact ttggtgctct cttttgtggg ccagacaaga
     2881 gttctcatgt taaatggaga ggaggtagaa gaaaccgaac tgatgggttt cgtggatgat
     2941 cagcagactt tcttctgtgg caacgtggct catcagcagc ttatccagat cacttcagca
     3001 tcggtgaggt tggtctctca agaacccaaa gctctggtca gtgaatggaa ggagcctcag
     3061 gccaagaaca tcagtgtggc ctcctgcaat agcagccagg tggtggtggc tgtaggcagg
     3121 gccctctact atctgcagat ccatcctcag gagctccggc agatcagcca cacagagatg
     3181 gaacatgaag tggcttgctt ggacatcacc ccattaggag acagcaatgg actgtcccct
     3241 ctttgtgcca ttggcctctg gacggacatc tcggctcgta tcttgaagtt gccttctttt
     3301 gaactactgc acaaggagat gctgggtgga gagatcattc ctcgctccat cctgatgacc
     3361 acctttgaga gtagccacta cctcctttgt gccttgggag atggagcgct tttctacttt
     3421 gggctcaaca ttgagacagg tctgttgagt gaccgtaaga aggtgacttt aggcacccag
     3481 cccaccgtat tgaggacttt tcgttctctt tctactacca acgtctttgc ttgctctgac
     3541 cgccccactg tcatctatag cagcaaccac aaattggtct tctcaaatgt caacctcaag
     3601 gaagtgaact acatgtgtcc cctcaattca gatggctatc ctgacagcct ggcactggcc
     3661 aacaatagca ccctcaccat tggcaccatc gacgagatcc agaagctgca cattcgcaca
     3721 gttcccctct atgaatctcc aaggaagatc tgctaccagg aagtgtccca gtgtttcggg
     3781 gtcctctcca gccgcattga agtccaagac acgagtgggg gcacaacagc cttgaggccc
     3841 agcgctagta cccaggctct ttccagcagt gtcagctcca gcaagctgtt ctccagcagc
     3901 actgctcctc atgagacctc ctttggagaa gaggtggagg tgcacaacct acttatcatt
     3961 gaccaacaca cctttgaagt gcttcatgcc caccagtttc tgcagaatga atatgccctc
     4021 agcctggtct cctgcaagct gggcaaagac cccaacactt acttcattgt gggcacagca
     4081 atggtgtatc ctgaagaggc agagcccaag cagggtcgca ttgtggtctt tcagtattcg
     4141 gatggaaaac tacagactgt ggctgaaaag gaagtgaaag gggccgtgta ctctatggtg
     4201 gaatttaacg ggaagctgtt agccagcatc aatagcacgg tgcggctcta tgagtggaca
     4261 acagagaagg agctgcgcac tgagtgcaac cactacaaca acatcatggc cctctacctg
     4321 aagaccaagg gcgacttcat cctggtgggc gaccttatgc gctcagtgct gctgctcgcc
     4381 tacaagccca tggaaggaaa ctttgaagag attgctcgag actttaatcc caactggatg
     4441 agtgctgtgg aaatcttgga tgatgacaat tttctggggg ccgaaaatgc ctttaacttg
     4501 tttgtgtgtc aaaaggacag cgctgccacc actgacgagg agcggcagca cctccaggag
     4561 gttggtcttt tccacctggg cgagtttgtc aatgtctttt gccacggctc tctggtaatg
     4621 cagaatctgg gtgagacttc cacccccaca caaggctcgg tgctctttgg cacggtcaat
     4681 ggcatgatag ggctggtgac ctcactgtca gagagctggt acaacctcct gctggacatg
     4741 cagaatcgtc tcaataaagt catcaaaagt gtggggaaga tcgagcactc cttctggaga
     4801 tcctttcaca ctgagcggaa gacagaacca gccacaggtt tcatcgatgg tgacttgatt
     4861 gagagtttcc tggatatcag ccgccccaag atgcaggagg tggtggcaaa cctacagtat
     4921 gacgatggca gcggtatgaa gcgagaggct actgcagacg acctcatcaa ggttgtggag
     4981 gagctaactc ggatccattg atgacgaagc ttgtcgagaa gtactagagg atcataatca
     5041 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     5101 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     5161 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5221 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactga
     5281 tatcgcctag gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt
     5341 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
     5401 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
     5461 cacagcgggg catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt
     5521 gacaaaccgt catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc
     5581 tcttcgttat taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc
     5641 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5701 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5761 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5821 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5881 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttctta
     5941 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6001 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6061 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6121 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6181 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6241 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6301 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6361 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6421 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6481 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6541 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6601 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6661 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6721 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6781 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6841 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6901 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6961 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7021 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7081 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7141 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7201 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7261 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7321 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7381 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     7441 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7501 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7561 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7621 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7681 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7741 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7801 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7861 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7921 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7981 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     8041 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag accagccgcg
     8101 taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta agcggg
//
