LOCUS       sequence-27541-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Evers TH, van Dongen EM, Faesen AC, Meijer EW, Merkx M
  TITLE     Quantitative understanding of the energy transfer between 
            fluorescent proteins connected via flexible peptide linkers.
  JOURNAL   Biochemistry. 2006 Nov 7. 45(44):13183-92.
  PUBMED    17073440
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2006 Nov 7. 45(44):13183-92."
COMMENT     Sequence Label: pET28CLY4
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     98..119
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(294..315)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     643..662
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
       61 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
      121 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtgct acctccggat
      181 ccaccactac caccggatcc accagatcca ccggatccac ctgatccacc ggatccacca
      241 cgaattccgg acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      301 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      361 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      421 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttggcc
      481 ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgtggctgat gtagttgtac
      541 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      601 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      661 tccttgaaga agatggtgcg ctcctggacg tagccttcg
//
