LOCUS       sequence-275489-       13353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lenti vector encoding the MS2-LSD1 with a 2A Hygro resistance 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13353)
  AUTHORS   Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson 
            KE, Ni M, Xu J
  TITLE     Interrogation of enhancer function by enhancer-targeting CRISPR 
            epigenetic editing.
  JOURNAL   Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5.
  PUBMED    31980609
REFERENCE   2  (bases 1 to 13353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-24"; volume: "11(1):485. doi"; pages: " 
            10.1038/s41467-020-14362-5"
COMMENT     Sequence Label: Lenti_MCP-LSD1_Hygro
FEATURES             Location/Qualifiers
     source          1..13353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2450..3628
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2681..3619
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3576..3596
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3635..3644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3644..4030
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             4085..4105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6692..6745
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6749..7768
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    7808..8396
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7861..7881)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8279..8290)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8399..8415)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8400..8416)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8921..9101
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9127..9144)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9133..9357
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9403..9831
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9490..9509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9700..9721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9840..9860)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9845..10174
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10025..10160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10087..10106
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10222..10269
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10288..10662
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10792..10913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10829..10848)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10883..10902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10962..10978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10962..10978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10975..10997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10986..11002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11010..11016,11017..11034,11035..11040))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11055..11076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11193..11210)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11364..11952)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11444..11463)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12123..12914,12915..12983))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12746..12765
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12984..13088)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13163..13182)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttagctagct gcaaagatgg ataaagtttt aaacagagag
     2401 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
     2461 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2521 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2581 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     2641 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     2701 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     2761 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     2821 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     2881 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     2941 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     3001 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     3061 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     3121 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     3181 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     3241 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatgaagga cgcggcgctc
     3301 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     3361 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     3421 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     3481 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     3541 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     3601 tttttttctt ccatttcagg tgtcgtgacg tacggccacc atggcttcaa actttactca
     3661 gttcgtgctc gtggacaatg gtgggacagg ggatgtgaca gtggctcctt ctaatttcgc
     3721 taatggggtg gcagagtgga tcagctccaa ctcacggagc caggcctaca aggtgacatg
     3781 cagcgtcagg cagtctagtg cccagaagag aaagtatacc atcaaggtgg aggtccccaa
     3841 agtggctacc cagacagtgg gcggagtcga actgcctgtc gccgcttgga ggtcctacct
     3901 gaacatggag ctcactatcc caattttcgc taccaattct gactgtgaac tcatcgtgaa
     3961 ggcaatgcag gggctcctca aagacggtaa tcctatccct tccgccatcg ccgctaactc
     4021 aggtatctac agcgctggag gaggtggaag cggaggagga ggaagcggag gaggaggtag
     4081 cggacctaag aaaaagagga aggtggcggc cgctggatcc atgttatctg ggaagaaggc
     4141 ggcagccgcg gcggcggcgg ctgcagcggc agcaaccggg acggaggctg gccctgggac
     4201 agcaggcggc tccgagaacg ggtctgaggt ggccgcgcag cccgcgggcc tgtcgggccc
     4261 agccgaggtc gggccggggg cggtggggga gcgcacaccc cgcaagaaag agcctccgcg
     4321 ggcctcgccc cccgggggcc tggcggaacc gccggggtcc gcagggcctc aggccggccc
     4381 tactgtcgtg cctgggtctg cgacccccat ggaaactgga atagcagaga ctccggaggg
     4441 gcgtcggacc agccggcgca agcgggcgaa ggtagagtac agagagatgg atgaaagctt
     4501 ggccaacctc tcagaagatg agtattattc agaagaagag agaaatgcca aagcagagaa
     4561 ggaaaagaag cttcccccac caccccctca agccccacct gaggaagaaa atgaaagtga
     4621 gcctgaagaa ccatcgggtg tggagggcgc agctttccag agccgacttc ctcatgaccg
     4681 gatgacttct caagaagcag cctgttttcc agatattatc agtggaccac aacagaccca
     4741 gaaggttttt cttttcatta gaaaccgcac actgcagttg tggttggata atccaaagat
     4801 tcagctgaca tttgaggcta ctctccaaca attagaagca ccttataaca gtgatactgt
     4861 gcttgtccac cgagttcaca gttatttaga gcgtcatggt cttatcaact tcggcatcta
     4921 taagaggata aaacccctac caactaaaaa gacaggaaag gtaattatta taggctctgg
     4981 ggtctcaggc ttggcagcag ctcgacagtt acaaagtttt ggaatggatg tcacactttt
     5041 ggaagccagg gatcgtgtgg gtggacgagt tgccacattt cgcaaaggaa actatgtagc
     5101 tgatcttgga gccatggtgg taacaggtct tggagggaat cctatggctg tggtcagcaa
     5161 acaagtaaat atggaactgg ccaagatcaa gcaaaaatgc ccactttatg aagccaacgg
     5221 acaagctgtt cctaaagaga aagatgaaat ggtagagcaa gagtttaacc ggttgctaga
     5281 agctacatct taccttagtc atcaactaga cttcaatgtc ctcaataata agcatgtgtc
     5341 ccttggccag gcattggaag ttgtcattca gttacaagag aagcatgtca aagatgagca
     5401 gattgaacat tggaagaaga tagtgaaaac tcaggaagaa ttgaaagaac ttcttaataa
     5461 gatggtaaat ttgaaagaga aaattaaaga actccatcag caatacaaag aagcatctga
     5521 agtaaagcca cccagagata ttactgccga gttcttagtg aaaagcaaac acagggatct
     5581 gaccgcccta tgcaaggaat atgatgaatt agctgaaaca caaggaaagc tagaagaaaa
     5641 acttcaggag ttggaagcga atcccccaag tgatgtatat ctctcatcaa gagacagaca
     5701 aatacttgat tggcattttg caaatcttga atttgctaat gccacacctc tctcaactct
     5761 ctcccttaag cactgggatc aggatgatga ctttgagttc actggcagcc acctgacagt
     5821 aaggaatggc tactcgtgtg tgcctgtggc tttagcagaa ggcctagaca ttaaactgaa
     5881 tacagcagtg cgacaggttc gctacacggc ttcaggatgt gaagtgatag ctgtgaatac
     5941 ccgctccacg agtcaaacct ttatttataa atgcgacgca gttctctgta cccttcccct
     6001 gggtgtgctg aagcagcagc caccagccgt tcagtttgtg ccacctctcc ctgagtggaa
     6061 aacatctgca gtccaaagga tgggatttgg caaccttaac aaggtggtgt tgtgttttga
     6121 tcgggtgttc tgggatccaa gtgtcaattt gttcgggcat gttggcagta cgactgccag
     6181 caggggtgag ctcttcctct tctggaacct ctataaagct ccaatactgt tggcactagt
     6241 ggcaggagaa gctgctggta tcatggaaaa cataagtgac gatgtgattg ttggccgatg
     6301 cctggccatt ctcaaaggga tttttggtag cagtgcagta cctcagccca aagaaactgt
     6361 ggtgtctcgt tggcgtgctg atccctgggc tcggggctct tattcctatg ttgctgcagg
     6421 atcatctgga aatgactatg atttaatggc tcagccaatc actcctggcc cctcgattcc
     6481 aggtgcccca cagccgattc cacgactctt ctttgcggga gaacatacga tccgtaacta
     6541 cccagccaca gtgcatggtg ctctgctgag tgggctgcga gaagcgggaa gaattgcaga
     6601 ccagtttttg ggggccatgt atacgctgcc tcgccaggcc acaccaggtg ttcctgcaca
     6661 gcagtcccca agcatgtgta caggcagtgg agagggcaga ggaagtctgc taacatgcgg
     6721 tgacgtcgag gagaatcctg gcccaaccat gaaaaagcct gaactcaccg ctacctctgt
     6781 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctccgaggg
     6841 cgaagaatct cgggctttca gcttcgatgt gggagggcgt ggatatgtcc tgcgggtgaa
     6901 tagctgcgcc gatggtttct acaaagatcg ctatgtttat cggcactttg catccgccgc
     6961 tctccctatt cccgaagtgc ttgacattgg ggagttcagc gagagcctga cctattgcat
     7021 ctcccgccgt gcacagggtg tcaccttgca agacctgcct gaaaccgaac tgcccgctgt
     7081 tctccagccc gtcgccgagg ccatggatgc catcgctgcc gccgatctta gccagaccag
     7141 cgggttcggc ccattcggac ctcaaggaat cggtcaatac actacatggc gcgatttcat
     7201 ctgcgctatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     7261 tgcctccgtc gcccaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     7321 ccggcacctc gtgcacgccg atttcggctc caacaatgtc ctgaccgaca atggccgcat
     7381 aacagccgtc attgactgga gcgaggccat gttcggggat tcccaatacg aggtcgccaa
     7441 catcttcttc tggaggccct ggttggcttg tatggagcag cagacccgct acttcgagcg
     7501 gaggcatccc gagcttgcag gatctcctcg gctccgggct tatatgctcc gcattggtct
     7561 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggctcaggg
     7621 tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc gggcgtacac aaatcgcccg
     7681 cagaagcgct gccgtctgga ccgatggctg tgtggaagtg ctcgccgata gtggaaacag
     7741 acgccccagc actcgtccta gggcaaagga tctgcagtaa tgagaattcg atatcaagct
     7801 tatcggtaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7861 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7921 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7981 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     8041 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     8101 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     8161 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     8221 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     8281 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     8341 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     8401 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     8461 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     8521 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8581 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     8641 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     8701 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     8761 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     8821 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     8881 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     8941 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     9001 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     9061 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     9121 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     9181 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     9241 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     9301 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     9361 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     9421 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9481 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9541 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9601 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9661 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9721 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9781 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     9841 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9901 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     9961 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10021 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10081 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
    10141 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
    10201 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
    10261 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
    10321 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
    10381 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
    10441 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
    10501 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
    10561 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
    10621 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
    10681 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    10741 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    10801 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    10861 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10921 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    10981 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    11041 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    11101 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    11161 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    11221 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    11281 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    11341 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    11401 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    11461 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    11521 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    11581 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    11641 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    11701 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    11761 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    11821 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11881 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11941 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    12001 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    12061 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    12121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    12181 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    12241 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    12301 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    12361 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    12421 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    12481 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    12541 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    12601 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    12661 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    12721 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    12781 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    12841 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    12901 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    12961 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    13021 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    13081 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
    13141 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
    13201 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
    13261 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
    13321 tgcttcgcga tgtacgggcc agatatacgc gtt
//
