LOCUS       sequence-275741-        9435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the TYK2-P1104A variant, rs34536443.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Li Z, Gakovic M, Ragimbeau J, Eloranta ML, Ronnblom L, Michel F, 
            Pellegrini S
  TITLE     Two rare disease-associated Tyk2 variants are catalytically impaired
            but signaling competent.
  JOURNAL   J Immunol. 2013 Mar 1;190(5):2335-44. doi: 10.4049/jimmunol.1203118.
            Epub 2013 Jan 28.
  PUBMED    23359498
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1203118"; journalName: "J Immunol"; date: 
            "2013-03-1- 1"; volume: "190"; issue: "5"; pages: "2335-44"
COMMENT     Sequence Label: pRC/CMV-TYK2P1104A-VSV
FEATURES             Location/Qualifiers
     source          1..9435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3858..3890
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     promoter        complement(3925..3943)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3926..3943)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3963..3980)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3969..4193
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4239..4752
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4326..4345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4536..4557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4775..4795)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4780..5109
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4960..5095
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5176..5970
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5230..5249)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5840..5859
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6144..6278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6181..6200)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6235..6254
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6309..6325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6309..6325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6322..6344)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6333..6349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6357..6363,6364..6381,6382..6387))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6402..6423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6540..6557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6711..7299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6791..6810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7470..8261,8262..8330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8093..8112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8331..8435)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8510..8529)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8701..9080
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9081..9284
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9235..9255
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9329..9348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9329..9347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccccaagaa gccgcgggga ctggctgcgc ttgacaggct gcacttggat gggagcacct
       61 ggtgcctcgg gactgctccg atgcccgggt ctgtgctgaa tgtgtaatat gcggaactat
      121 attgaaacat tacaaccatc ttttgatggc aacaccctga ggacctccct tttccagatg
      181 gggaaactga ggcccagaat tgctaagtgg cttgcttgag ttgacacagg gagctccagg
      241 actcaccctc agctgagcca cctgccggga gcatgcctct gcgccactgg gggatggcca
      301 ggggcagtaa gcccgttggg gatggagccc agcccatggc tgccatggga ggcctgaagg
      361 tgcttctgca ctgggctggt ccaggcggcg gggagccctg ggtcactttc agtgagtcat
      421 cgctgacagc tgaggaagtc tgcatccaca ttgcacataa agttggtatc actcctcctt
      481 gcttcaatct ctttgccctc ttcgatgctc aggcccaagt ctggttgccc ccaaaccaca
      541 tcctagagat ccccagagat gcaagcctga tgctatattt ccgcataagg ttttatttcc
      601 ggaactggca tggcatgaat cctcgggaac cggctgtgta ccgttgtggg cccccaggaa
      661 ccgaggcatc ctcagatcag acagcacagg ggatgcaact cctggaccca gcctcatttg
      721 agtacctctt tgagcagggc aagcatgagt ttgtgaatga cgtggcatca ctgtgggagc
      781 tgtcgaccga ggaggagatc caccacttta agaatgagag cctgggcatg gcctttctgc
      841 acctctgtca cctcgctctc cgccatggca tccccctgga ggaggtggcc aagaagacca
      901 gcttcaagga ctgcatcccg cgctccttcc gccggcatat ccggcagcac agcgccctga
      961 cccggctgcg ccttcggaac gtcttccgca ggttcctgcg ggacttccag ccgggccgac
     1021 tctcccagca gatggtcatg gtcaaatacc tagccacact cgagcggctg gcaccccgct
     1081 tcggcacaga gcgtgtgccc gtgtgccacc tgaggctgct ggcccaggcc gagggggagc
     1141 cctgctacat ccgggacagt ggggtggccc ctacagaccc tggccctgag tctgctgctg
     1201 ggcccccaac ccacgaggtg ctggtgacag gcactggtgg catccagtgg tggccagtag
     1261 aggaggaggt gaacaaggag gagggttcta gtggcagcag tggcaggaac ccccaagcca
     1321 gcctgtttgg gaagaaggcc aaggctcaca aggcattcgg ccagccggca gacaggccgc
     1381 gggagccact gtgggcctac ttctgtgact tccgggacat cacccacgtg gtgctgaaag
     1441 agcactgtgt cagcatccac cggcaggaca acaagtgcct ggagctgagc ttgccttccc
     1501 gggctgcggc gctgtccttc gtgtcgctgg tggacggcta tttccgcctg acggccgact
     1561 ccagccacta cctgtgccac gaggtggctc ccccacggct ggtgatgagc atccgggatg
     1621 ggatccacgg acccctgctg gagccatttg tgcaggccaa gctgcggccc gaggacggcc
     1681 tgtacctcat tcactggagc accagccacc cctaccgcct gatcctcaca gtggcccagc
     1741 gtagccaggc accagacggc atgcagagct tgcggctccg aaagttcccc attgagcagc
     1801 aggacggggc cttcgtgctg gagggctggg gccggtcctt ccccagcgtt cgggaacttg
     1861 gggctgcctt gcagggctgc ttgctgaggg ccggggatga ctgcttctct ctgcgtcgct
     1921 gttgcctgcc ccaaccagga gaaacctcca atctcatcat catgcggggg gctcgggcca
     1981 gccccaggac actcaacctc agccagctca gcttccaccg ggttgaccag aaggagatca
     2041 cccagctgtc ccacttgggc cagggcacaa ggaccaacgt gtatgagggc cgcctgcgag
     2101 tggagggcag cggggaccct gaggagggca agatggatga cgaggacccc ctcgtgcctg
     2161 gcagggaccg tgggcaggag ctacgagtgg tgctcaaagt gctggaccct agtcaccatg
     2221 acatcgccct ggccttctac gagacagcca gcctcatgag ccaggtctcc cacacgcacc
     2281 tggccttcgt gcatggcgtc tgtgtgcgcg gccctgaaaa tatcatggtg acagagtacg
     2341 tggagcacgg acccctggat gtgtggctgc ggagggagcg gggccatgtg cccatggctt
     2401 ggaagatggt ggtggcccag cagctggcca gcgccctcag ctacctggag aacaagaacc
     2461 tggttcatgg taatgtgtgt ggccggaaca tcctgctggc ccggctgggg ttggcagagg
     2521 gcaccagccc cttcatcaag ctgagtgatc ctggcgtggg cctgggcgcc ctctccaggg
     2581 aggagcgggt ggagaggatc ccctggctgg cccccgaatg cctaccaggt ggggccaaca
     2641 gcctaagcac cgccatggac aagtgggggt ttggcgccac cctcctggag atctgctttg
     2701 acggagaggc ccctctgcag agccgcagtc cctccgagaa ggagcatttc taccagaggc
     2761 agcaccggct gcccgagccc tcctgcccac agctggccac actcaccagc cagtgtctga
     2821 cctatgagcc aacccagagg ccatcattcc gcaccatcct gcgtgacctc acccggctgc
     2881 agccccacaa tcttgctgac gtcttgactg tgaacccgga ctcaccggcg tcggacccta
     2941 cggttttcca caagcgctat ttgaaaaaga tccgagatct gggcgagggt cacttcggca
     3001 aggtcagctt gtactgctac gatccgacca acgacggcac tggcgagatg gtggcggtga
     3061 aagccctcaa ggcagactgc ggcccccagc accgctcggg ctggaagcag gagattgaca
     3121 ttctgcgcac gctctaccac gagcacatca tcaagtacaa gggctgctgc gaggaccaag
     3181 gcgagaagtc gctgcagctg gtcatggagt acgtgcccct gggcagcctc cgagactacc
     3241 tgccccggca cagcatcggg ctggcccagc tgctgctctt cgcccagcag atctgcgagg
     3301 gcatggccta tctgcacgcg cagcactaca tccaccgaga cctagccgcg cgcaacgtgc
     3361 tgctggacaa cgacaggctg gtcaagatcg gggactttgg cctagccaag gccgtgcccg
     3421 aaggccacga gtactaccgc gtgcgcgagg atggggacag ccccgtgttc tggtatgccc
     3481 cagagtgcct gaaggagtat aagttctact atgcgtcaga tgtctggtcc ttcggggtga
     3541 ccctgtatga gctgctgacg cactgtgact ccagccagag cgcccccacg aaattccttg
     3601 agctcatagg cattgctcag ggtcagatga cagttctgag actcactgag ttgctggaac
     3661 gaggggagag gctgccacgg cccgacaaat gtccctgtga ggtctatcat ctcatgaaga
     3721 actgctggga gacagaggcg tcctttcgcc caaccttcga gaacctcata cccattctga
     3781 agacagtcca tgagaagtac caaggccagg ccccttcagt gttcagcgtg tgcccccggg
     3841 agggcccacc aggcccatac accgacatcg agatgaaccg gctgggcaag taaagatcta
     3901 aggtaccaat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     3961 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4021 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4081 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     4141 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     4201 ggcggaaaga accagctggg gctcgagggg ggatccccac gcgccctgta gcggcgcatt
     4261 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4321 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4381 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4441 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4501 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4561 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4621 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4681 aacgcttaca atttaaatat ttgcttatac aatcttcctg tttttggggc ttttctgatt
     4741 atcaaccggg gtgggtaccg agctcgaatt ctgtggaatg tgtgtcagtt agggtgtgga
     4801 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4861 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4921 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4981 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5041 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5101 ttttgcaaaa agctcccggg agcttggata tccattttcg gatctgatca agagacagga
     5161 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5221 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5281 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5341 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5401 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5461 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5521 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5581 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5641 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5701 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5761 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5821 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5881 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5941 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     6001 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     6061 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6121 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6181 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6241 tgtccaaact catcaatgta tcttatcatg tctggatccg tcgacctcga gagcttggcg
     6301 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6361 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6421 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6481 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8461 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     8521 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     8581 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     8641 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     8701 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     8761 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     8821 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     8881 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     8941 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     9001 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     9061 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     9121 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     9181 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     9241 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
     9301 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
     9361 gtcgaggaac tgaaaaacca gaaagttaac tggtaagttt agtctttttg tcttttattt
     9421 caggtcccgg atccc
//
