LOCUS       sequence-275752-        9574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Tei R, Baskin JM
  TITLE     Spatiotemporal control of phosphatidic acid signaling with 
            optogenetic, engineered phospholipase Ds.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133675. doi: 
            10.1083/jcb.201907013.
  PUBMED    31999306
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133675. doi"; 
            pages: " 10.1083/jcb.201907013"
COMMENT     Sequence Label: Plasma membrane-targeted optoPLD
FEATURES             Location/Qualifiers
     source          1..9574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             761..2254
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWRKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             2288..2992
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2430..2448)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2698..2717
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4607..4663
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4670..5179
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     primer_bind     complement(5292..5309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5298..5522
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5655..5674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5806..5853
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5861..6883
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5861..6883
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7013..7134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7050..7069)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7104..7123
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7183..7199)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7183..7199)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7196..7218)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7207..7223
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7231..7237,7238..7255,7256..7261))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7276..7297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7414..7431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7585..8173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7665..7684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8344..9135,9136..9204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8967..8986
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9205..9309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9384..9403)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtacgccacc atgaagatgg acaaaaagac
      781 tatagtttgg tttagaagag acctaaggat tgaggataat cctgcattag cagcagctgc
      841 tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag gacagtttta
      901 tcctggaaga gcttcaagat ggtggatgaa acaatcactt gctcacttat ctcaatcctt
      961 gaaggctctt ggatctgacc tcactttaat caaaacccac aacacgattt cagcgatctt
     1021 ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct atgatcctgt
     1081 ttcgttagtt cgggaccata ccgtaaagga gaagctggtg gaacgtggga tctctgtgca
     1141 aagctacaat ggagatctat tgtatgaacc gtgggagata tactgcgaaa agggcaaacc
     1201 ttttacgagt ttcaattctt actggaggaa atgcttagat atgtcgattg aatccgttat
     1261 gcttcctcct ccttggcggt tgatgccaat aactgcagcg gctgaagcga tttgggcgtg
     1321 ttcgattgaa gaactagggc tggagaatga ggccgagaaa ccgagcaatg cgttgttaac
     1381 tagagcttgg tctccaggat ggagcaatgc tgataagtta ctaaatgagt tcatcgagaa
     1441 gcagttgata gattatgcaa agaacagcaa gaaagttgtt gggaattcta cttcactact
     1501 ttctccgtat ctccatttcg gggaaataag cgtcagacac gttttccagt gtgcccggat
     1561 gaaacaaatt atatgggcaa gagataagaa cagtgaagga gaagaaagtg cagatctttt
     1621 tcttagggga atcggtttaa gagagtattc tcggtatata tgtttcaact tcccgtttac
     1681 tcacgagcaa tcgttgttga gtcatcttcg gtttttccct tgggatgctg atgttgataa
     1741 gttcaaggcc tggagacaag gcaggaccgg ttatccgttg gtggatgccg gaatgagaga
     1801 gctttgggct accggatgga tgcataacag aataagagtg attgtttcaa gctttgctgt
     1861 gaagtttctt ctccttccat ggaaatgggg aatgaagtat ttctgggata cacttttgga
     1921 tgctgatttg gaatgtgaca tccttggctg gcagtatatc tctgggagta tccccgatgg
     1981 ccacgagctt gatcgcttgg acaatcccgc gttacaaggc gccaaatatg acccagaagg
     2041 tgagtacata aggcaatggc ttcccgagct tgcgagattg ccaactgaat ggatccatca
     2101 tccatgggac gctcctttaa ccgtactcaa agcttctggt gtggaactcg gaacaaacta
     2161 tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta ctagctaaag ctatttcaag
     2221 aacccgtgaa gcacagatca tgatcggagc agcaggtgga ggcggttcag gcggaggtgg
     2281 cagcggcgtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     2341 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2401 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     2461 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     2521 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2581 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2641 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2701 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2761 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2821 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2881 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2941 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agagatctcg
     3001 aagcgcggcc gcgggagcag gaggagcagc tcgaggcgca gcagcgggag caggaggagc
     3061 aggtcgatcg ggcggatctg cagattcagc tacaccacat ttggacgccg ttgagcaaac
     3121 attgaggcaa gtgtccccag gcctggaggg cgatgtatgg gaaagaacta gtggaaataa
     3181 attggacgga agtgccgctg atcccagtga ttggttgttg caaaccccag gatgttgggg
     3241 cgatgataaa tgcgctgaca gagttgggac aaaaaggttg ctggctaaga tgactgaaaa
     3301 tatagggaat gccaccagaa ccgtagatat tagtacactt gcacctttcc caaacggcgc
     3361 attccaagac gctattgtcg ctggtttgaa ggagtccgca gctaaaggca ataagttgaa
     3421 agttaggata ctggttgggg ctgcacccgt ctaccacatg aatgtaatac cttccaagta
     3481 tagggacgaa ttgactgcaa aacttggcaa agccgcagaa aacataactt tgaacgtggc
     3541 tagtatgact acatcaaaaa cagctttctc ctggaaccat agcaaaatct tggttgtaga
     3601 cgggcaaagt gcccttactg gtggaataaa tagctggaag gatgattact tggatactac
     3661 tcatccagtg tctgatgttg atcttgcact gaccggacca gctgcaggct ctgccggtag
     3721 ataccttgac actttgtgga catggacctg ccaaaacaag agtaacatcg cttccgtctg
     3781 gtttgcagct tccggcaacg cagggtgcat gcccactatg cataaagata caaaccccaa
     3841 ggcatcacct gccaccggga atgtcccagt aatagccgtt ggagggttgg gcgttggcat
     3901 caaagatgtg gaccccaagt ccactttcag gcccgatctg ccaacagcta gcgatactaa
     3961 gtgtgtggta ggacttcacg acaatactaa cgccgataga gactatgata cagtcaaccc
     4021 agaagagagt gccttgagag ctcttgttgc ttcagcaaaa ggtcacattg aaatatctca
     4081 gcaggatttg aacgctacat gtccaccctt gcctaggtat gacattagat tgtacgatgc
     4141 cttggctgct aaaatggctg ctggggttaa ggtgagaatt gtggttagcg acccagccaa
     4201 caggggcgca gtgggcagtg ggggttacag ccaaataaaa tcacttagtg aaatatcaga
     4261 cactttgaga aacaggcttg caaatatcac aggcggtcaa caagctgcca aaactgcaat
     4321 gtgtagcaat ttgcagcttg caacctttag atcctccccc aacggcaaat gggcagacgg
     4381 gcatccttat gcccaacacc ataagttggt ttccgtcgac tcatccacat tttatatcgg
     4441 ttcaaaaaat ttgtacccat cctggctgca agattttggc tacatagtgg aatccccaga
     4501 ggctgccaag caactggatg ccaaattgct tgaccctcag tggaagtact cacaggagac
     4561 tgcaacagtg gattacgcaa gaggaatctg caacgctgga agcggcgcta caaactttag
     4621 tctgctcaag caggctggcg atgtcgagga aaacccaggt cccggtacca tgaatggagc
     4681 tataggaggt gaccttttgc tcaattttcc tgacatgtcg gtcctagagc gccaaagggc
     4741 tcacctcaag tacctcaatc ccacctttga ttctcctctc gccggcttct ttgccgattc
     4801 ttcaatgatt accggcggcg agatggacag ctatctttcg actgccggtt tgaatcttcc
     4861 gatgatgtac ggtgagacga cggtggaagg tgattcaaga ctctcaattt cgccggaaac
     4921 gacgcttggg actggaaatt tcaaggcagc gaagtttgat acagagacta aggattgtaa
     4981 tgaggcggcg aagaagatga cgatgaacag agatgaccta gtagaagaag gagaagaaga
     5041 gaagtcgaaa ataacagagc aaaacaatgg gagcacaaaa agcatcaaga agatgaaaca
     5101 caaagccaag aaagaagaga acaatttctc taatgattca tctaaagtga cgaaggaatt
     5161 ggagaaaacg gattatattc atgtaccggt cgccaccgga tcaggatcac tgtataaggg
     5221 taaaaagaag aaaaagaagt caaagacaaa gtgtgtaatt atgtaggggc ccgtttaaac
     5281 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5341 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5401 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5461 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5521 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     5581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5641 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5761 cctcgacccc aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg
     5821 aagttcctat tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc
     5881 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     5941 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     6001 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     6061 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     6121 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     6181 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     6241 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     6301 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     6361 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     6421 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     6481 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     6541 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     6601 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     6661 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     6721 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     6781 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     6841 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt
     6901 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6961 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     7021 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7081 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7141 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     7201 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7261 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7321 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7381 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7441 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7501 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7561 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7621 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7681 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7741 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7801 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7861 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7921 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7981 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8041 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8101 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8161 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8221 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8281 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8341 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8401 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8461 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8521 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8581 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8641 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8701 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8761 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8821 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8881 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8941 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9001 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9061 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     9361 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     9421 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     9481 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     9541 ctgcttcgcg atgtacgggc cagatatacg cgtt
//
