LOCUS       sequence-275762-        3905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, CPH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P223
FEATURES             Location/Qualifiers
     source          1..3905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    345..400
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     promoter        1626..1730
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2798..3386
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3287..3306
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3540..3557
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggaaacacca atgacgtgta ccttgagact gatacgattc aaagggatag aaacacgtca
       61 gatttacaag tggcacctct tcaatggaca atgggtattc caatatgcta agatgctacg
      121 agatatctaa tttatctaac acaactcaat tccaaaccaa aaatctgatg ccagctcgac
      181 aagacaaaaa atctaagctc aaaaatgtca acaaccaata gaaatcaagg cattgacgat
      241 atcacgagat aagcaaatta aatcttcaag ttttgcaatt catatgtacg ttataaatac
      301 ccaaaaacct caccgtaacc tagctatcct actgtatttt tacaacaatt accaacaaca
      361 acaaacaaca aacaacatta caattactat ttacaattac aatggctcct atctcctcca
      421 cctggtccag attaatcagg ttcgtagcta tagaaacgtc actcgtgcat ataggcgaac
      481 cgattgatgc aactatggac gtagggcttg ctcgtcgaga agggaaaact attcaggcct
      541 atgagattat cggatctggc agcgccctgg acctttctgc acaggtgagt aagaacgtgc
      601 tcacagtacg agaattgttg atgccacttt ctagagaaga gataaagacg gtcagatgct
      661 taggccttaa ctatcctgtc cacgcaacag aagccaatgt tgctgtaccg aagtttccga
      721 acctgttcta caaaccagtc acaagtctca tcgggcctga tggtcttatc acgatcccta
      781 gtgtagtgca accgcctaag gaacatcagt cagattatga agctgaactt gtgattgtaa
      841 tcggaaaggc cgccaagaac gtgtctgagg acgaagcctt ggattatgtc ttaggctata
      901 cagctgctaa cgatattagt ttccgaaaac accagctcgc agtctcacag tggtcattct
      961 caaaaggatt tggatctctc cttctcacta tacgtatggc ccagacacat agtggaaaca
     1021 taaataggtt ctcacgtgat caaatcttta atgttaagaa aacaatcagc tttttgtctc
     1081 aaggaactac cctcgaacct gggtctatca ttttgactgg cacccctgat ggggtcggct
     1141 tcgtcaggaa cccgccctta tacttaaaag atggggatga agtgatgact tggataggtt
     1201 ccggcattgg tacgcttgct aacaccgttc aagaagagaa aacatgcttt gcaagcggtg
     1261 ggcatgagta ggcttttatg ttggtgatat ggtggtaaat gtagggattt agtttacaat
     1321 tgcgtatgtc tgtgttggat atctgtagtg ctgttcttat ggcttagatc ttgtaatttc
     1381 tcattacagt atcaatgaat agatatcagt ttctagtgat gacattggtt cgtcttttag
     1441 ctgttgatta atttttctta attgattcat cctattgcaa ttcttctgaa tttaaattgt
     1501 atactgtgaa attaagaaaa ttcttgaaat taatgagaat ttgagtaata gcgctgttag
     1561 agctctgacg tgggcaggtg tgatgaaaag ccttagggtc aggtggcact tttcggggaa
     1621 atgtgcgatg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctaa
     1681 tgagacaata accctgataa atgcttcaat tatattgaaa aaggaagagt atgagtatcc
     1741 aacatttccg tgtcgccctt attccctttt ttgcggcctt ctgccttcct gtttttgctc
     1801 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc cgagtgggtt
     1861 acattgaact ggatctcaac agcggcaaaa tcttagagag ttttcgcccc gaagaacgct
     1921 tcccaatgat gagcactttc aaagttctgc tatgtggcgc ggtgttatcc cgtattgatg
     1981 ctgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtatt
     2041 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgtagtgctg
     2101 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt ggaggaccga
     2161 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     2221 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     2281 tggcaacaac tctccgcaaa ctattaactg gcgaactact tactcttgct tcacgccagc
     2341 aactcattga ctggatggag gcggacaaag ttgcaggacc acttctgcgc tcggcacttc
     2401 cggctggctg gtttattgct gataaatctg gagccggtga gcgtggctct cgcggtatca
     2461 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2521 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2581 agcattggta actgtcagac gaagggaaat aaatagtagc ccgcctgatg tgcgggcttt
     2641 tttttggtgg tggttaccag tgtcgcttag ctaccgtagg tcctcttagg cggtccgatt
     2701 agcgacatag tcatcaatgc cttgacggca aaatccctta acgtgagttt tcgttccact
     2761 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2821 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2881 aagagctact aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2941 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3001 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3061 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3121 ggggttcgtg ctcacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3181 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3241 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3301 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3361 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3421 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3481 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3541 gcgagtcagt gagcgaggaa gcggaagagc gcccacctgc tcatgatcgg cgcgccctat
     3601 ggagatatga ctggtaagtt tttcttgcca atacgaatta gaaaacatgt ctttgaagat
     3661 gaactgtatt tttttttttt actttgttgt cattttaatg tactttctta tcaggattaa
     3721 atcttctgta atttagagta gtttttttaa caagataatt aacaaactta gagtaatgaa
     3781 aattgagatg ttcagttttc actcatattt cacattttgg tgaaagagtg ggtagtatgc
     3841 aacgttctaa gtatgtttgg actttgtatc atgttgtttt gattctttga cgacatgtct
     3901 atttg
//
