LOCUS       sequence-27578-e         328 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Krek W, Ewen ME, Shirodkar S, Arany Z, Kaelin WG, Livingston DM
  TITLE     Negative regulation of the growth-promoting transcription factor 
            E2F-1 by a stably bound cyclin A-dependent protein kinase.
  JOURNAL   Cell. 1994 Jul 15. 78(1):161-72.
  PUBMED    8033208
REFERENCE   2  (bases 1 to 328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1994 
            Jul 15. 78(1):161-72."
COMMENT     Sequence Label: HA-E2F-1 wt-pRcCMV
FEATURES             Location/Qualifiers
     source          1..328
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             88..114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttac catggcctac ccctacgacg tgcccgacta cgcctccctc
      121 ggatcccata tggccttggc cggggcccct gcgggcggcc catgcgcgcc ggcgctggag
      181 gccctgctcg gggccggcgc gctgcggctg ctcgactcct cgcagatcgt catcatctcc
      241 gccgcgcagg acgccagcgc cccgccggct cccaccggcc ccgcggcgcc cgccgccggc
      301 ccctgcgacc ctgacctgct gctcttcg
//
