LOCUS       sequence-275858-       15777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein hSfi1 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15777)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 15777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Sfi1
FEATURES             Location/Qualifiers
     source          1..15777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..96)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    85..196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5074..5092)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5160..5264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5265..5333,5334..6125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5483..5502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6296..6884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6785..6804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7073..7093)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7091..7388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7465..8064
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7465..7484)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7961..7981
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8155..8289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8427..8446)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8723..8984
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          9101..9673
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     9681..9700
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     9728..9747
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9728..9746
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9757..10377
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9830..9849)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     polyA_signal    10419..10540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        10595..10974
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10975..11178
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11129..11149
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    11180..11198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11201..11219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11229..11253
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             11353..11376
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             11377..11394
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             11944..11967
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    11980..12004
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(15767..15777,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatggc agctagcggc gcgcccggcc gctcgagtct agagggcccg
       61 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      241 ggcaggacag caagggggag gattgggaag acaatagcag gctagaggat cgatccccgc
      301 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
      361 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
      421 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
      481 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
      541 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
      601 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
      661 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
      721 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
      781 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
      841 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
      901 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
      961 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     1021 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     1081 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     1141 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     1201 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     1261 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     1321 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     1381 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     1441 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     1501 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     1561 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     1621 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     1681 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     1741 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     1801 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     1861 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     1921 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     1981 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     2041 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2101 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     2161 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     2221 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     2281 gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta gagtgggagt
     2341 gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct gcttgtcctt
     2401 ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa agccgtcgca
     2461 tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc
     2521 gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg ggaggacgaa
     2581 aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt ctactgggga
     2641 tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg tctcctaaca
     2701 agttacatca ctcctgccct tcctcaccct catctccatc acctccttca tctccgtcat
     2761 ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag ggaggcaaat
     2821 ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga gcgggctttg
     2881 tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga gtttgtaaaa
     2941 agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg ccgggtccag
     3001 gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga atagcaaggg
     3061 cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg aacacaccgg
     3121 cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg agagctctta
     3181 aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga tgctttccaa
     3241 accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca gtgcttgggc
     3301 cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg tcaggctcac
     3361 catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca gggtggaaaa
     3421 atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat gactgactac
     3481 tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct ctttcacgac
     3541 ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct cctcgacccc
     3601 ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg cctccacctc
     3661 ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     3721 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3781 ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcccc
     3841 ctcctcctcc tgcccctcct scccctccts ctcctgcccc tcctgcccct cctgctcctg
     3901 cccctccygc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgcccctc
     3961 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct cctgcccctc
     4021 ctgctcctgc ccctcctnnn gctcctgccc ctcctgcnnn tcctgcccct cctccygctc
     4081 ctgcccctcc tgcccctcct cctgctcctg cccctmctcc tgctcctgcc cctcctgcsc
     4141 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctnnn gctcctgccc
     4201 ctcctcctgc tcctscycct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc
     4261 ctgctcctgc ccctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     4321 ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc tgctcctgct cctgttccac
     4381 cgtgggtccc tttgcagcca atgcaacttg gacgtttttg gggtctccgg acaccatctc
     4441 tatgtcttgg ccctgatcct gagccgcccg gggctcctgg tcttccgcct cctcgtcctc
     4501 gtcctcttcc ccgtcctcgt ccatggttat caccccctct tctttgaggt ccactgccgc
     4561 cggagccttc tggtccagat gtgtctccct tctctcctag gccatttcca ggtcctgtac
     4621 ctggcccctc gtcagacatg attcacacta aaagagatca atagacatct ttattagacg
     4681 acgctcagtg aatacaggga gtgcagactc ctgccccctc caacagcccc cccaccctca
     4741 tccccttcat ggtcgctgtc agacagatcc aggtctgaaa attccccatc ctccgaacca
     4801 tcctcgtcct catcaccaat tactcgcagc ccggaaaact cccgctgaac atcctcaaga
     4861 tttgcgtcct gagcctcaag ccaggcctca aattcctcgt cccccttttt gctggacggt
     4921 agggatgggg attctcggga cccctcctct tcctcttcaa ggtcaccaga cagagatgct
     4981 actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat cgatgataag
     5041 ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     5101 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5161 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5221 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5281 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5341 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5401 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5461 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     5521 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5581 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5641 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5701 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5761 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     5821 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5881 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5941 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6001 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6061 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6121 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6181 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6241 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6421 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6901 cctttttacg gttcctggcc ttttgctggc cttgaagctg tccctgatgg tcgtcatcta
     6961 cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa
     7021 tcataatggg gaaggccatc cagcctcgcg tcgaactaga tgatccggct gtggaatgtg
     7081 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7141 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7201 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7261 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7321 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7381 ttttttggag ggtgaccgcc acgaccggtg ccgccaccat cccctgaccc acgcccctga
     7441 cccctcacaa ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc
     7501 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7561 acgcgccaca ccgtcgaccc cgaccgccac atcgaacgcg tcaccgagct gcaagaactc
     7621 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7681 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7741 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7801 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7861 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7921 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7981 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8041 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     8101 agcgcacgac ccggtccgac gcggcccgac gggtccgagg ggggtcgacc tcgaaacttg
     8161 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8221 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8281 gtctggatct gatccgaacc ccttcctcga ccaattctca tgtttgacag cttatcatcg
     8341 cagatccggg caacgttgtt gccattgctc gaccaattct catgtttgac agcttatcat
     8401 cgcagatccg tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8461 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     8521 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     8581 gcgctgcttc gcgatgtacg ggccagatat acgcgtatct gaggggacta gggtgtgttt
     8641 aggcgaaaag cggggcttcg gttgtacgcg gttaggagtc ccctcaggat atagtagttt
     8701 cgcttttgca tagggagggg gaaatgtagt cttatgcaat actcttgtag tcttgcaaca
     8761 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     8821 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     8881 ggattggacg aaccactgaa ttccgcattg cagagatatt gtatttaagt gcctagctcg
     8941 atacaataaa cgccatttga ccattcacca cattggtgtg cacctccaag ctggccgggg
     9001 atcctctagg gcctctgagc tattccagaa gtagtgaaga ggcttttttg gaggcctagg
     9061 cttttgcaaa aagctccgga tcgatcctga gaacttcagg gtgagtttgg ggacccttga
     9121 ttgttctttc tttttcgcta ttgtaaaatt catgttatat ggagggggca aagttttcag
     9181 ggtgttgttt agaatgggaa gatgtccctt gtatcaccat ggaccctcat gataattttg
     9241 tttctttcac tttctactct gttgacaacc attgtctcct cttattttct tttcattttc
     9301 tgtaactttt tcgttaaact ttagcttgca tttgtaacga atttttaaat tcacttttgt
     9361 ttatttgtca gattgtaagt actttctcta atcacttttt tttcaaggca atcagggtat
     9421 attatattgt acttcagcac agttttagag aacaattgtt ataattaaat gataaggtag
     9481 aatatttctg catataaatt ctggctggcg tggaaatatt cttattggta gaaacaacta
     9541 catcctggtc atcatcctgc ctttctcttt atggttacaa tgatatacac tgtttgagat
     9601 gaggataaaa tactctgagt ccaaaccggg cccctctgct aaccatgttc atgccttctt
     9661 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     9721 agaattgtaa tacgactcac tatagggcga attgatatgt ctagattaga taaaagtaaa
     9781 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
     9841 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
     9901 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
     9961 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
    10021 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
    10081 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
    10141 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
    10201 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
    10261 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
    10321 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgcg
    10381 tacagcggat cccgggaatt cagatcttat taaagcagaa cttgtttatt gcagcttata
    10441 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10501 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg tcgaatccat
    10561 ggcgcagaac tggtaggtat ggaagatccg cgttgacatt gattattgac tagttattaa
    10621 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
    10681 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
    10741 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
    10801 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
    10861 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
    10921 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
    10981 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
    11041 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
    11101 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
    11161 gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat
    11221 cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
    11281 gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag
    11341 cttaccacca tggattataa agacgatgat gacaagcacc accaccacca ccacgacaag
    11401 gattgtgaaa tgaaacgcac cacactggac agccctttgg ggaagctgga gctgtctggt
    11461 tgtgagcagg gtctgcacga aataattttt ctgggcaagg ggacgtctgc agctgatgcc
    11521 gtggaggtcc cagcccccgc tgcggttctc ggaggtccgg agcccctgat gcaggctaca
    11581 gcctggctga atgcctattt ccaccagccc gaggctatcg aagagttccc cgtgccggct
    11641 cttcaccatc ccgttttcca gcaagagtcg ttcaccagac aggtgttatg gaagctgctg
    11701 aaggttgtga aattcggaga agtgatttct tactctcact tagcagccct ggcaggcaac
    11761 cccgcagcca cggcagcagt gaagacggca ctgagtggca atcctgtccc tatcctgatc
    11821 ccgtgccaca gagtggtcca gggggatttg gatgtgggcg gttacgaggg tggactggcc
    11881 gtgaaggaat ggcttctggc ccatgaaggc caccggttgg ggaagccagg cttgggattc
    11941 gaactggagg tgctgttcca gggccccggt accgccatca caagtttgta caaaaaagca
    12001 ggcttgaagg aattcggtac catgaagaat ctgctcactg agaagtgtat atcaagccac
    12061 aatttccatc aaaaagtgat taagcagaga atggagaaga aggttgattc caggtatttt
    12121 aaggatggtg cagttaagaa accttattct gcaaagacac tgtccaacaa gaagtcttct
    12181 gcatcctttg ggatccggag ggagttacct agtaccagtc atctagtgca gtatcgtggc
    12241 acacatactt gtacccgaca gggccggtta agagaactgc gcatcagatg cgtggccaga
    12301 aagttcttat atttatggat tcgaatgact tttggaagag tatttccctc taaagccaga
    12361 ttttactatg agcagcgatt actacggaag gtcttcgaag aatggaaaga ggagtggtgg
    12421 gttttccagc acgagtggaa actctgtgtt cgagctgact gtcactacag gtattacctg
    12481 tacaacctga tgttccagac gtggaagacc tatgtgcgtc agcagcagga gatgaggaac
    12541 aagtacatta gagccgaggt tcatgatgca aagcaaaaga tgcgacaggc ctggaagtcc
    12601 tggttgatct acgtggttgt tcgtaggacc aaacttcaga tgcagactac agctctggag
    12661 tttaggcaac ggattatctt acgggtgtgg tggagcacgt ggaggcagcg actaggacag
    12721 gtccgtgtga gccgtgccct ccatgcctct gctttgaagc acagggccct gagcctccag
    12781 gtgcaggctt ggtcacagtg gcgggaacag ctcctgtatg tccagaagga gaaacaaaag
    12841 gttgtctctg cagtgaaaca tcatcagcac tggcaaaaac ggagatttct aaaggcctgg
    12901 cttgaatacc tgcaagtccg cagagtgaag agacagcaga atgagatggc tgagcgattc
    12961 catcatgtca ctgtgctcca gatatacttc tgtgactggc agcaggcctg ggagcggagg
    13021 gagagcttgt acgctcacca tgcccaggtg gagaaactgg ccaggaagat ggccctgcgg
    13081 cgcgccttta ctcactggaa acactacatg ctgctgtgtg cagaagaagc tgcccagttt
    13141 gagatggcag aagagcacca caggcacagc cagctgtatt tttgctttag agccctaaaa
    13201 gacaatgtga cccacgctca tctccagcaa ataagaagga atcttgctca ccagcagcat
    13261 ggtgtcacgc tgctgcacag gttctggaac ctctggcggt ctcagattga gcagaaaaag
    13321 gaaagagagc tgctcccctt actgcatgct gcctgggacc actacagaat agcactgctg
    13381 tgcaaatgta tcgaattgtg gctacagtat actcagaaga ggcggtacaa gcagctgcta
    13441 caggccagag cggatggtca tttccagcag agagccctgc ctgctgcctt ccacacatgg
    13501 aacagactct ggcgatggcg ccaccaggaa aatgtcctca gtgcaagagc aacacgtttc
    13561 cacagggaga cattagagaa gcaagtattt tctctctgga ggcagaagat gtttcagcat
    13621 cgagaaaacc gcctggcaga gagaatggcc atccttcacg cagagcgaca gcttctgtat
    13681 aggtcttggt tcatgtggca ccagcaggca gcagcacgtc accaggagca ggagtggcaa
    13741 acagtggcct gtgcccacca ccgccacggg cggctcaaga aagctttctg cctctggagg
    13801 gaaagtgccc aagggctcag aacagagagg acgggcaggg tgcgggcagc agaattccac
    13861 atggcccagc tcctgcgttg ggcctggagc cagtggaggg agtgcctggc cctgcgggga
    13921 gcggagcggc agaagctgat gcgagcagac ctgcaccacc agcacagcgt gctgcacagg
    13981 gcgctgcagg catgggtgac ttaccagggc agggtgcgaa gcatcctccg ggaggtggca
    14041 gccagggaga gccagcacaa caggcagctg ctgcgcgggg cattacgtcg ctggaaagag
    14101 aacaccatgg cccgagtgga tgaagccaaa aaaacctttc aagcaagtac tcattacaga
    14161 aggaccatat gttccaaggt cctggtccag tggcgggaag ctgtgtcagt gcagatgtat
    14221 taccgacagc aggaggactg tgccatctgg gaggcccaga aggtgctgga caggggctgt
    14281 ctgcggacct ggtttcagcg ctggtgggac tgcagccgga ggtcagccca gcagagactg
    14341 cagctggaga gggcagtgca acaccaccac cggcagctgc tgctggaggg gctggcccgg
    14401 tggaagacgc accatctgca gtgtgtcagg aagaggctcc tgcacaggca gagcacccaa
    14461 ctgctggcac agagactcag ccggacctgc ttccgccagt ggagacaaca gctggcagcc
    14521 aggaggcagg agcagcgggc gacagtgcgg gccctgtggt tctgggcctt ctcgctgcag
    14581 gcaaaggtgt gggccacgtg gctggccttt gtactggaaa ggaggagaaa gaaggcgcgg
    14641 ctgcagtggg cgctccaggc ctaccagggg cagctcctcc aggagggtgc cacgcggctc
    14701 ctgcgctttg cagccagcat gaaggcctcc cggcagcagc tgcaggccca gcagcaggtc
    14761 caggcggctc acagtctcca tcgtgccgtc cgccgctgtg ccacgctctg gaaacagaaa
    14821 gtgctgggcc ggggcgggaa gcctcagccc ctggcagcca tcgcacccag caggaaagtg
    14881 acgtttgagg gtccccttct caaccgcatt gctgctgggg ctggggatgg cacccttgag
    14941 accaagaggc cacaggcttc tcggcctctg ggagctctgg gccgcctggc tgctgaggag
    15001 ccccacgccc tggagctcaa cactgcccac tcagcgagga agcagccgcg acgcccacac
    15061 ttcctgttgg agcctgcgca gagccagagg cctcagaagc cacaggaaca tggcctaggc
    15121 atggctcagc cagcagcccc ctccctgacg cggcccttcc tggcagaggc cccgacagca
    15181 ctggtcccac acagccccct gcctggggcc ctgtcaagcg cccctggccc gaagcagccc
    15241 ccgacggcaa gcacaggccc ggagctgctg ctgctgcctc tttcctcctt catgccctgc
    15301 ggggcggctg caccagccag ggtgtcagca cagcgggcta ctcctaggga taagcccccg
    15361 gtcccctcat ccctggccag tgtccctgac ccccatctac tccttcctgg ggacttctca
    15421 gccaccaggg ctgggcctgg actttcaact gcaggcagcc tggaccttga ggctgaactt
    15481 gaggagatcc agcagcaact actgcactac cagaccacca agcagaacct ctggtcctgt
    15541 cggcggcaag cgagcagcct gcgcaggtgg ctggagctga acagagagga gccagggcct
    15601 gaggaccagg aagtagagca gcaggtgcag aaagagctgg aacaggtgga aatgcagatc
    15661 cagctgctgg cagaggagct ccaggctcag cgccagccca ttggcgcctg cgttgcccgc
    15721 atccaggccc tgcggcaggc cctgtgctag ctcgagtgcg gccgcaaccc agctttc
//
