LOCUS       sequence-276139-         486 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses 
            GB1-His6-TEV-ALIX(800-868)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Elias RD, Ma W, Ghirlando R, Schwieters CD, Reddy VS, Deshmukh L
  TITLE     Proline-rich domain of human ALIX contains multiple TSG101-UEV 
            interaction sites and forms phosphorylation-mediated reversible 
            amyloids.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Sep 11. pii: 2010635117. doi: 
            10.1073/pnas.2010635117.
  PUBMED    32917811
REFERENCE   2  (bases 1 to 486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 Sep 11. pii: 2010635117. doi: 
            10.1073/pnas.2010635117."
COMMENT     Sequence Label: GB1-PRD(800-868)
FEATURES             Location/Qualifiers
     source          1..486
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             166..183
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             184..195
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             235..252
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             253..273
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cagtacaaac tggcgctgaa cggcaaaacg ctgaaaggtg agaccaccac cgaagcggtt
       61 gatgcggcga ccgccgaaaa ggtgttcaaa cagtacgcca acgacaacgg tgtggatggc
      121 gaatggacct acgatgacgc caccaaaacg ttcaccgtga ccgaactggt tccgcgcggt
      181 agcattgaag gccgcatccg cgaaaatctg aaagatagcg gtctgtttct ggaacatcat
      241 caccaccatc acgaaaatct gtacttccaa ggcggcggtc cgccgtatcc aacctacccg
      301 ggttacccgg gctactgcca gatgccgatg ccaatgggct acaacccgta cgcctacggc
      361 cagtacaata tgccgtaccc gccggtgtac catcagagtc cgggccaagc cccatatccg
      421 ggtccacagc agccaagtta cccgtttccg cagccgccgc agcagagtta ctatccgcag
      481 cagtaa
//
