LOCUS       sequence-276311-       13156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression  cassette for FLPe recombinase in T-DNA vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Cody JP, Graham ND, Zhao C, Swyers NC, Birchler JA
  TITLE     Site-specific recombinase genome engineering toolkit in maize.
  JOURNAL   Plant Direct. 2020 Mar 9;4(3):e00209. doi: 10.1002/pld3.209. 
            eCollection 2020 Mar.
  PUBMED    32166212
REFERENCE   2  (bases 1 to 13156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-03-9"; volume: "4(3):e00209. doi"; pages: " 
            10.1002/pld3.209. eCollection 2020 Mar"
COMMENT     Sequence Label: pTF-FLPe
FEATURES             Location/Qualifiers
     source          1..13156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1217..1846
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2275..3348
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3414..3608
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3844..3866)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3952..4092
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4007..4026
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4107..4124)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4278..4866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4358..4377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5112..5903)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6424..6448
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6648..6670
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6662..6679
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6663..6679
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7249..7797)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7949..8293)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7985..8005)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        8391..10387
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             10417..11691
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRIG"
     terminator      11866..12118
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      12477..12729
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12746..12762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12746..12762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12759..12781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12770..12786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12794..12800,12801..12818,12819..12824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12839..12860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    13018..13042
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttaa agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat
       61 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
      121 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
      181 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
      241 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
      301 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
      361 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
      421 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
      481 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
      541 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
      601 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
      661 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
      721 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
      781 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
      841 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
      901 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
      961 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     1021 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     1081 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     1141 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     1201 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     1261 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     1321 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     1381 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     1441 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     1501 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     1561 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     1621 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     1681 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     1741 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     1801 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     1861 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     1921 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     1981 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     2041 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     2101 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     2161 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     2221 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     2281 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     2341 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     2401 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     2461 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     2521 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     2581 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     2641 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     2701 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     2761 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     2821 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     2881 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     2941 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     3001 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     3061 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     3121 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     3181 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     3241 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     3301 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     3361 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     3421 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     3481 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     3541 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     3601 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     3661 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     3721 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     3781 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     3841 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     3901 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     3961 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     4021 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     4081 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4141 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4201 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4261 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4321 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4681 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     4741 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4801 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4861 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4921 cgttaaggga ttttggtcat gcatgatata tctcccaatt tgtgtagggc ttattatgca
     4981 cgcttaaaaa taataaaagc agacttgacc tgatagtttg gctgtgagca attatgtgct
     5041 tagtgcatct aacgcttgag ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattt
     5101 ctagctagac attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat
     5161 tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt
     5221 ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca
     5281 gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta
     5341 agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt
     5401 tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct
     5461 ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg
     5521 tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat
     5581 tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg
     5641 acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg
     5701 ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca
     5761 atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc
     5821 aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact
     5881 tcggcgatca ccgcttcccc catgatgttt aactttgttt tagggcgact gccctgctgc
     5941 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     6001 ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc
     6061 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
     6121 atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc
     6181 cgaattgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
     6241 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
     6301 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
     6361 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     6421 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     6481 gcggacgttt ttaatgtact gaattaacgc cgaattgctc tagcattcgc cattcaggct
     6541 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     6601 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     6661 ttgtaaaacg acggccagtg ccaagctaat tcgcttcaag acgtgctcaa atcactattt
     6721 ccacacccct atatttctat tgcactccct tttaactgtt ttttattaca aaaatgccct
     6781 ggaaaatgca ctcccttttt gtgtttgttt ttttgtgaaa cgatgttgtc aggtaattta
     6841 tttgtcagtc tactatggtg gcccattata ttaatagcaa ctgtcggtcc aatagacgac
     6901 gtcgattttc tgcatttgtt taaccacgtg gattttatga cattttatat tagttaattt
     6961 gtaaaaccta cccaattaaa gacctcatat gttctaaaga ctaatactta atgataacaa
     7021 ttttctttta gtgaagaaag ggataattag taaatatgga acaagggcag aagatttatt
     7081 aaagccgcgt aagagacaac aagtaggtac gtggagtgtc ttaggtgact tacccacata
     7141 acataaagtg acattaacaa acatagctaa tgctcctatt tgaatagtgc atatcagcat
     7201 accttattac atatagatag gagcaaactc tagctagatt gttgagcaga tctcggtgac
     7261 gggcaggacc ggacggggcg gtaccggcag gctgaagtcc agctgccaga aacccacgtc
     7321 atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag
     7381 cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt
     7441 gaagccctgt gcctccaggg acttcagcag gtgggtgtag agcgtggagc ccagtcccgt
     7501 ccgctggtgg cggggggaga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg
     7561 tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag
     7621 ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg
     7681 ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc
     7741 cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatggt
     7801 agatcccccg ttcgtaaatg gtgaaaattt tcagaaaatt gcttttgctt taaaagaaat
     7861 gatttaaatt gctgcaatag aagtagaatg cttgattgct tgagattcgt ttgttttgta
     7921 tatgttgtgt tgagaattaa ttctcgaggt cctctccaaa tgaaatgaac ttccttatat
     7981 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
     8041 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
     8101 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt caacgatggc
     8161 ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca ctatcttcac
     8221 aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata ttaccctttg
     8281 ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat ttttggagta
     8341 gacaagtgtg tcgtgctcca ccatgttgac cggcatgcaa gcttgcatgc ctgcagtgca
     8401 gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa
     8461 aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca
     8521 tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt
     8581 ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga gtattttgac
     8641 aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat
     8701 agcttcacct atataatact tcatccattt tattagtaca tccatttagg gtttagggtt
     8761 aatggttttt atagactaat ttttttagta catctatttt attctatttt agcctctaaa
     8821 ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa
     8881 taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa
     8941 acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac
     9001 ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca
     9061 tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg
     9121 ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc
     9181 ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc
     9241 tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc
     9301 tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca
     9361 cctccgcttc aaggtacgcc gctcgtcctc cccccccccc cctctctacc ttctctagat
     9421 cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt ttgtgttaga
     9481 tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca
     9541 gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta
     9601 gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat agggtttggt
     9661 ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat
     9721 gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag
     9781 tagaattaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     9841 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     9901 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     9961 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
    10021 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
    10081 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
    10141 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
    10201 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
    10261 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
    10321 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
    10381 tctgcaggtc gactctagag gatcccccgc gccaccatga gccaatttga tatattatgt
    10441 aaaacaccac ctaaggtcct ggttcgtcag tttgtggaaa ggtttgaaag accttcaggg
    10501 gaaaaaatag catcatgtgc tgctgaacta acctatttat gttggatgat tactcataac
    10561 ggaacagcaa tcaagagagc cacattcatg agctataata ctatcataag caattcgctg
    10621 agtttcgata ttgtcaacaa atcactccag tttaaataca agacgcaaaa agcaacaatt
    10681 ctggaagcct cattaaagaa attaattcct gcttgggaat ttacaattat tccttacaat
    10741 ggacaaaaac atcaatctga tatcactgat attgtaagta gtttgcaatt acagttcgaa
    10801 tcatcggaag aagcagataa gggaaatagc cacagtaaaa aaatgcttaa agcacttcta
    10861 agtgagggtg aaagcatctg ggagatcact gagaaaatac taaattcgtt tgagtatacc
    10921 tcgagattta caaaaacaaa aactttatac caattcctct tcctagctac tttcatcaat
    10981 tgtggaagat tcagcgatat taagaacgtt gatccgaaat catttaaatt agtccaaaat
    11041 aagtatctgg gagtaataat ccagtgttta gtgacagaga caaagacaag cgttagtagg
    11101 cacatatact tctttagcgc aaggggtagg atcgatccac ttgtatattt ggatgaattt
    11161 ttgaggaact ctgaaccagt cctaaaacga gtaaatagga ccggcaattc ttcaagcaac
    11221 aaacaggaat accaattatt aaaagataac ttagtcagat cgtacaacaa ggctttgaag
    11281 aaaaatgcgc cttatccaat ctttgctata aagaatggcc caaaatctca cattggaaga
    11341 catttgatga cctcatttct gtcaatgaag ggcctaacgg agttgactaa tgttgtggga
    11401 aattggagcg ataagcgtgc ttctgccgtg gccaggacaa cgtatactca tcagataaca
    11461 gcaatacctg atcactactt cgcactagtt tctcggtact atgcatatga tccaatatca
    11521 aaggaaatga tagcattgaa ggatgagact aatccaattg aggagtggca gcatatagaa
    11581 cagctaaagg gtagtgctga aggaagcata cgataccccg catggaatgg gataatatca
    11641 caggaggtac tagactacct ttcatcctac ataaatagac gcataggatg agctagtgca
    11701 tgcattcatc aatattattc atgcggggaa aggcaagatt aatccaactg gcaaatcatc
    11761 cagcgtgatt ggtaacttca gttccagcga cttgattcgt tttggtgcta cccacgtttt
    11821 caataaggac gagatggtgg agtaaagaag gagtgcgtcg aagcagatcg ttcaaacatt
    11881 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
    11941 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
    12001 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
    12061 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga
    12121 tcaaacttcg gcactgtgta atgacgatga gcaatcgaga ggctgactaa caaaaggtat
    12181 gcccaaaaac aacctctcca aactgtttcg aattggaagt ttctgctcat gccgacaggc
    12241 ataacttaga tattcgcggg ctattcccac taattcgtcc tgctggtttg cgccaagata
    12301 aatcagtgca tctccttaca agttcctctg tcttgtgaaa tgaactgctg actgcccccc
    12361 aagaaagcct cctcatctcc cagttggcgg cggctgatac accatcgaaa acccacgtcc
    12421 gaacacttga tacatgtgcc tgagaaatag gggtcgaccc gagctcgaat ttccccgatc
    12481 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
    12541 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
    12601 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
    12661 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
    12721 tactagatcg ggaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
    12781 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    12841 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    12901 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    12961 gagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
    13021 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
    13081 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
    13141 ttcccctcgg gatcaa
//
