LOCUS       sequence-276416-        5298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of CD44 proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Mielgo A, van Driel M, Bloem A, Landmann L, Gunthert U
  TITLE     A novel antiapoptotic mechanism based on interference of Fas 
            signaling by CD44 variant isoforms.
  JOURNAL   Cell Death Differ. 2006 Mar;13(3):465-77. doi: 
            10.1038/sj.cdd.4401763.
  PUBMED    16167069
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/sj.cdd"; 
            journalName: "Cell Death Differ"; date: "2006-03"; volume: "13"; 
            issue: "3"; pages: "465-77"
COMMENT     Sequence Label: pPGK-T7/2-CD44v9 (-cyt)
FEATURES             Location/Qualifiers
     source          1..5298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..500
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(36..59)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     433..452
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     intron          550..1122
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1130..1149
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1177..1196
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1177..1195
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2195..2204
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2201..2203,2204..2206,2207..2920)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2201..2203,2204..2206,2207..2917)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2507..2526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2854..2875
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2954..3075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2991..3010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3045..3064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3117..3221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3222..3290,3291..4082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3440..3459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4253..4841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4742..4761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4995..5012
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5166..5185)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
       61 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
      121 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
      181 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
      241 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
      301 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
      361 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
      421 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
      481 cctcatctcc gggcctttcg acccctaggc ttttgcaaaa agctccggat cgatcctgag
      541 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      601 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      661 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      721 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      781 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      841 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      901 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      961 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
     1021 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
     1081 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1141 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
     1201 ttccgctccg gacaccatgg acaagttttg gtggcacgca gcctggggac tctgcctcgt
     1261 gccgctgagc ctggcgcaga tcgatttgaa tataacctgc cgctttgcag gtgtattcca
     1321 cgtggagaaa aatggtcgct acagcatctc tcggacggag gccgctgacc tctgcaaggc
     1381 tttcaatagc accttgccca caatggccca gatggagaaa gctctgagca tcggatttga
     1441 gacctgcagg tatgggttca tagaagggca cgtggtgatt ccccggatcc accccaactc
     1501 catctgtgca gcaaacaaca caggggtgta catcctcaca tccaacacct cccagtatga
     1561 cacatattgc ttcaatgctt cagctccacc tgaagaagat tgtacatcag tcacagacct
     1621 gcccaatgcc tttgatggac caattaccat aactattgtt aaccgtgatg gcacccgcta
     1681 tgtccagaaa ggagaataca gaacgaatcc tgaagacatc taccccagca accctactga
     1741 tgatgacgtg agcagcggct cctccagtga aaggagcagc acttcaggag gttacatctt
     1801 ttacaccttt tctactgtac accccatccc agacgaagac agtccctgga tcaccgacag
     1861 cacagacaga atccctgcta ccaagcagag taattctcag agcttctcta catcacatga
     1921 aggcttggaa gaagataaag accatccaac aacttctact ctgacatcaa gcagagacca
     1981 agacacattc caccccagtg gggggtccca taccactcat ggatctgaat cagatggaca
     2041 ctcacatggg agtcaagaag gtggagcaaa cacaacctct ggtcctataa ggacacccca
     2101 aattccagaa tggctgatca tcttggcatc cctcttggcc ttggctttga ttcttgcagt
     2161 ttgcattgca gtcaacagag atccccggcc ggtcgccacc atggtgagca agggcgagga
     2221 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2281 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2341 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2401 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2461 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2521 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2581 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2641 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2701 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2761 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2821 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2881 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg agatccagat
     2941 cttattaaag cagaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3061 catcaatgta tcttatcatg tctggtcgag caggtggcac ttttcgggga aatgtgcgcg
     3121 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3181 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3241 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3301 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3361 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3421 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3481 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3541 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3601 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3661 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3721 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3781 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3841 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3901 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3961 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4021 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4081 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4141 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4201 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4261 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4321 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4381 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4441 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4501 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4561 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4621 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4681 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4741 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4801 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4861 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     4921 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4981 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5041 cgcctctccc cgcgcgttgg ccgattcatt tcgacttctg aggcggaaag aaccaggcca
     5101 atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5161 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5221 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5281 tgaatgaaca attctacc
//
