LOCUS       sequence-276430-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for human ACE2 with an N-terminal 
            (extracellular) c-myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pCEP4-myc-ACE2
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             111..140
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagcgcca
       61 ccatgaagac catcatcgcc ctgagctaca tcttctgcct ggtgttcgcc gagcaaaagc
      121 tgatctcaga ggaggacctg ggcagccctg gcggtgcctc caccattgag gaacaggcca
      181 agacattttt ggacaagttt aaccacgaag ccgaagacct gttctatcaa agttcacttg
      241 cttcttggaa ttataacacc aatattactg aagagaatgt ccaaaacatg aataatgctg
      301 gggacaaatg gtctgccttt ttaaaggaac agtccacact tgcccaaatg tatccactac
      361 aagaaattca gaatctcaca gtcaagcttc agctgcaggc tcttcagcaa aatgggtctt
      421 cagtgctctc agaagacaag agcaaacggt tgaacacaat tctaaataca atgagcacca
      481 tctacagtac tggaaaagtt tgtaacccag ataatccaca agaatgctta ttacttgaac
      541 caggtttgaa tgaaataatg gcaaacagtt tagactacaa tgagaggctc tgggcttggg
      601 aaagctggag atctgaggtc ggcaagcagc tgaggccatt atatgaagag tatgtggtct
      661 tgaaaaatga gatggcaaga gcaaatcatt atgaggacta tggggattat tggagaggag
      721 actatgaagt aaatggggta gatggctatg actacagccg cggccagttg attgaagatg
      781 tggaacatac ctttgaagag attaaaccat tatatgaaca tcttcatgcc tatgtgaggg
      841 caaagttgat gaatgcctat ccttcctata tcagtccaat tggatgcctc cctgctcatt
      901 tgcttggtga tatgtggggt agattttgga caaatctgta ctctttgaca gttccctttg
      961 g
//
