LOCUS       sequence-276447-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP4
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ttgggtatta ctgcagcacg gcgctggtga
      121 tagaggtctg agggggctgg tacagcacgt cggatccgct gttagagaag tcgttcagag
      181 ccttggccag gtggcagcaa gcggcttcac ggtaagaggt ggtgtccata gcgccggaga
      241 agtacttgta cttgttgtac agggccaggt acctgttgta ctgggtcaga ggcagcagca
      301 cgtcgctgcg cagcttcagg tacatctcct tgttcagcag gaaggtgcac agagcggctt
      361 cctcgaaggt ggagaagctc acgccgttga acaccacacg tctcttcagg tagttgctga
      421 agaaccagta gaagtgcttg gtagagatgc agatgatgta agcgatggtg atccagaaag
      481 gcaccagagg ggtgaacatg accatccact ggatgtgggc caggaaagac acgtcgttgg
      541 tcaggtagaa ggtcaggtac aggtagatca cgctgtacac gcctggcagg aaagagtaca
      601 cgggggtcag gcacagcacg gtgaaggaca tcaggaacag cagggtgttg aaagccacca
      661 cgtggctgta ttcaccgaag gccctgcgga acctcatgaa gtagtaagcc aggcaggtca
      721 ccacgatggc cacgatacct ccagccacga tagaggcgga gatgtccaga gcaccgatgg
      781 gctggatcag tggggtgaac atgttggtca gcaggttcac agcatccact ccgcagaaca
      841 cgccaggcag agacctgtag tagtcgttgt tcagcaccca gcgaccgctg gtagacacgc
      901 acactccggc ttcggaacgc tcgcaggttc cgtgtctgca gtattcggag tcgaa
//
