LOCUS       sequence-276449-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF9B gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 ORF9B
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(391..490)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(506..523)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(506..522)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(515..537)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      586..632
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tacaagaaag ttgggtatta cttcacggtc accaccacga
      121 attcgtcggg cagttcctcg gtggtggcca gcttggtcat ctgcacagcg attggggtca
      181 gctggaaggc cttgtcctcc aggctgttca gggtcttacg ggccatgttc agggacagag
      241 ggctgcccag tctcaggatg atagggtaca ccttgggtcc cacgttgttc tggtccctgc
      301 ccacagcgtt ttccatgcgg gtcacggcca gctggatctg agggtccacc agtctcagag
      361 cggggtgcat ttcagagatc tttgggtcca tgccaacttt tttgtacaaa gttggcatta
      421 taaaaaagca ttgctcatca atttgttgca acgaacaggt cactatcagt caaaataaaa
      481 tcattatttg gggcccgagc ttaagactgg ccgtcgtttt acaacgtcgt gactgggaaa
      541 acatccatgc tagcgttaac gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
      601 gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tg
//
