LOCUS       sequence-276454-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF7A gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 ORF7A
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(461..560)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      613..699
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 cttataatgc caactttgta caagaaagtt gggtattatt cggtcttacg cttcagggtg
      121 aagcacaggg tgatgaacac gatagcggcc acgatcagga agatggggct gtacagctcc
      181 tgcacttcct cctgtctgat gaacagcttt gggctcacag acctggcgcg cagctggtac
      241 acgtgcttca caccatcagg gcaagcgaag gcgaactgag tagagaagca ggtcagagcg
      301 aacttgttgt cggccagagg gtggaagggg gagtttcctt cgtaggtgcc ggagctgcaa
      361 ggctccttca gcagcacggt ggtaccgcgc acgcattcct ggtagtggta cagctcgcag
      421 gtagccaggg tgatcagggc caggaacagg atgatcttca tgccaacttt tttgtacaaa
      481 gttggcatta taaaaaagca ttgctcatca atttgttgca acgaacaggt cactatcagt
      541 caaaataaaa tcattatttg gggcccgaga tccatgctag cgttaacgcg agagtaggga
      601 actgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt tcgttttatc
      661 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      721 gttgtgaagc aacggcccgg agggtggcg
//
