LOCUS       sequence-276464-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP5
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    68..92
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gattttattt tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct
       61 tataatgcca actttgtaca agaaagttgg gtattactgg aaggtcacac cagagcactg
      121 cctcaccacg tcgaaggggg tgaattcgtc ctccagcaga gcggatccca ggatggtgcg
      181 tccgttcatg ccgttctgca gcagttcctt cagggaggcg cacatgtcca gcacagcgat
      241 tccagtctgg gcgctcagtg gacccaggat atccacgtgg tcctgggtca gaggctcgta
      301 gttgtacttc atagccacca ggttgaagtc gttcagggtg gtggtgaacc tgttcaggaa
      361 ccagcggtct ccgttgatca cagcggcgta cagccaagcc agcacgttca cggtgatggt
      421 ggtgtcggtg ccagcagcct gagcagtctg tctgtccacg aaggggccgt agaagttacc
      481 ctccaggtca gttccagcgt gcactccagt tggcagctcc atgtggtgca tgtagcagaa
      541 ggacacgcag tcgtagtcga tgttgaaacc cacgcttccg caagagccgt tcaggaagct
      601 gcccttgatg gtgaagttgg gacgcatagc gcactggtac acgccggatg ggcttccgtt
      661 gtagcaggcc agcacggaga aggtctgacc aggctggatt ctcacgaact tgtacttagg
      721 ggtcttgggg ttagcggtgt ccaccttcag cttcagcacg cagttctgca tagagtggcc
      781 gatcacacgc agctgcacgt taccggcctg
//
