LOCUS       sequence-27673-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Ron D, Dressler H
  TITLE     pGSTag--a versatile bacterial expression plasmid for enzymatic 
            labeling of recombinant proteins.
  JOURNAL   Biotechniques. 1992 Dec . 13(6):866-9.
  PUBMED    1476738
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 1992 Dec . 13(6):866-9."
COMMENT     Sequence Label: pGSTag
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(149..166)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(193..215)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cgcgaggcag ancgtcagtc agtcacgatg aattaagctt gagctcgagt cgacccatgg
       61 agtctagaat tccaccacca ccaccggaaa ttcccgggga tcctttacgg taactcggac
      121 gacggctcag aatttcgcga cgttctgcag atccacgcgg aaccagatcc gattttggag
      181 gatggtcgcc accaccaaac gtggcttgcc agccctgcaa aggccatgct atatacttgc
      241 tggatttcaa gtacttatca atttgtggga tagcttcaat acgtttttta aaacaaacta
      301 attttgggaa cgcatccagg cacattgggt ccatgtataa aacaacatca agagcgtcat
      361 acaacatgaa gtcaggatgg gttacatgat caccatttaa atatgtttta tgacataaac
      421 gatcttcgaa cattttcagc atttcaggta gcttgctaag aaaatcaact ttgagagttt
      481 caaagtcttt actatatgca attctcgaaa caccgtatct aatatccaaa accgctcctt
      541 caagcattga aatctctgca cgctcttttg gacaaccacc caacatgttg tgcttgtcag
      601 ctatataacg tatgatggcc atagactgtg ttaatttaac atcaccatca anataat
//
