LOCUS       sequence-276760-        1757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the RELA transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0174
FEATURES             Location/Qualifiers
     source          1..1757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1291..1662
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 accgccacca tggacgaact gttccccctc atcttcccgg cagagccagc ccaggcctct
       61 ggcccctatg tggagatcat tgagcagccc aagcagcggg gcatgcgctt ccgctacaag
      121 tgcgaggggc gctccgcggg cagcatccca ggcgagagga gcacagatac caccaagacc
      181 caccccacca tcaagatcaa tggctacaca ggaccaggga cagtgcgcat ctccctggtc
      241 accaaggacc ctcctcaccg gcctcacccc cacgagcttg taggaaagga ctgccgggat
      301 ggcttctatg aggctgagct ctgcccggac cgctgcatcc acagtttcca gaacctggga
      361 atccagtgtg tgaagaagcg ggacctggag caggctatca gtcagcgcat ccagaccaac
      421 aacaacccct tccaagttcc tatagaagag cagcgtgggg actacgacct gaatgctgtg
      481 cggctctgct tccaggtgac agtgcgggac ccatcaggca ggcccctccg cctgccgcct
      541 gtcctttctc atcccatctt tgacaatcgt gcccccaaca ctgccgagct caagatctgc
      601 cgagtgaacc gaaactctgg cagctgcctc ggtggggatg agatcttcct actgtgtgac
      661 aaggtgcaga aagaggacat tgaggtgtat ttcacgggac caggctggga ggcccgaggc
      721 tccttttcgc aagctgatgt gcaccgacaa gtggccattg tgttccggac ccctccctac
      781 gcagacccca gcctgcaggc tcctgtgcgt gtctccatgc agctgcggcg gccttccgac
      841 cgggagctca gtgagcccat ggaattccag tacctgccag atacagacga tcgtcaccgg
      901 attgaggaga aacgtaaaag gacatatgag accttcaaga gcatcatgaa gaagagtcct
      961 ttcagcggac ccaccgaccc ccggcctcca cctcgacgca ttgctgtgcc ttcccgcagc
     1021 tcagcttctg tccccaagcc agcaccccag ccctatccct ttacgtcatc cctgagcacc
     1081 atcaactatg atgagtttcc caccatggtg tttccttctg ggcagatcag ccaggcctcg
     1141 gccttggccc cggcccctcc ccaagtcctg ccccaggctc cagcccctgc ccctgctcca
     1201 gccatggtat cagctctggc ccaggcccca gcccctgtcc cagtcctagc cccaggccct
     1261 cctcaggctg tggccccacc tgcccccaag cccacccagg ctggggaagg aacgctgtca
     1321 gaggccctgc tgcagctgca gtttgatgat gaagacctgg gggccttgct tggcaacagc
     1381 acagacccag ctgtgttcac agacctggca tccgtcgaca actccgagtt tcagcagctg
     1441 ctgaaccagg gcatacctgt ggccccccac acaactgagc ccatgctgat ggagtaccct
     1501 gaggctataa ctcgcctagt gacaggggcc cagaggcccc ccgacccagc tcctgctcca
     1561 ctgggggccc cggggctccc caatggcctc ctttcaggag atgaagactt ctcctccatt
     1621 gcggacatgg acttctcagc cctgctgagt cagatcagct cctagtaatg aactagtccg
     1681 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaacttacc
     1741 gcttagggac gtatgta
//
