LOCUS       sequence-277192-        1145 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0756
FEATURES             Location/Qualifiers
     source          1..1145
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaacacc cttctacggc gatgaggcgc tgagcggcct gggcggcggc
       61 gccagtggca gcggcggcag cttcgcgtcc ccgggccgct tgttccccgg ggcgcccccg
      121 acggccgcgg ccggcagcat gatgaagaag gacgcgctga cgctgagcct gagtgagcag
      181 gtggcggcag cgctcaagcc tgcggccgcg ccgcctccta cccccctgcg cgccgacggc
      241 gcccccagcg cggcaccccc cgacggcctg ctcgcctctc ccgacctggg gctgctgaag
      301 ctggcctccc ccgagctcga gcgcctcatc atccagtcca acgggctggt caccaccacg
      361 ccgacgagct cacagttcct ctaccccaag gtggcggcca gcgaggagca ggagttcgcc
      421 gagggcttcg tcaaggccct ggaggattta cacaagcaga accagctcgg cgcgggcgcg
      481 gccgctgccg ccgccgccgc cgccgccggg gggccctcgg gcacggccac gggctccgcg
      541 ccccccggcg agctggcccc ggcggcggcc gcgcccgaag cgcctgtcta cgcgaacctg
      601 agcagctacg cgggcggcgc cgggggcgcg gggggcgccg cgacggtcgc cttcgctgcc
      661 gaacctgtgc ccttcccgcc gccgccaccc ccaggcgcgt tggggccgcc gcgcctggct
      721 gcgctcaagg acgagccaca gacggtgccc gacgtgccga gcttcggcga gagcccgccg
      781 ttgtcgccca tcgacatgga cacgcaggag cgcatcaagg cggagcgcaa gcggctgcgc
      841 aaccgcatcg ccgcctccaa gtgccgcaag cgcaagctgg agcgcatctc gcgcctggaa
      901 gagaaagtga agaccctcaa gagtcagaac acggagctgg cgtccacggc gagcctgctg
      961 cgcgagcagg tggcgcagct caagcagaaa gtcctcagcc acgtcaacag cggctgccag
     1021 ctgctgcccc agcaccaggt gcccgcgtac tagtaatgaa ctagtccgta acttgaaagt
     1081 atttcgattt cttggcttta tatatcttgt ggaaaggacg agaatgatgc tccttgtatc
     1141 actta
//
