LOCUS       sequence-277306-        1694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0829
FEATURES             Location/Qualifiers
     source          1..1694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtagcaa cagtgggcag gctggccgcc acatctataa atccctagct
       61 gatgatggcc cctttgactc tgtggagccg cctaaaagac ccaccagcag actcatcatg
      121 cacagcatgg ccatgttcgg aagagagttc tgctacgcgg tggaggcagc gtatgtgacc
      181 ccagtcctgc tcagcgtagg tctgcccagc agcctgtaca gcattgtgtg gttcctcagc
      241 cccatcctgg gattcctgct gcagcccgtg gtcggatcgg ccagcgacca ctgccggtcc
      301 aggtggggcc gccggagacc ctacatcctc accctgggag tcatgatgct cgtgggcatg
      361 gctctgtacc tcaatggggc tactgttgta gcagctttga ttgctaaccc aaggaggaag
      421 ctggtttggg ccataagtgt caccatgata ggtgtcgttc tctttgattt tgctgccgac
      481 ttcattgatg ggcccatcaa agcctactta tttgatgtct gctcccatca ggacaaggag
      541 aagggcctcc actaccatgc cctcttcaca ggttttggag gtgccctggg ttaccttttg
      601 ggtgctatag actgggccca tctggagctg ggaagactgt tgggtacaga attccaggtc
      661 atgttcttct tctctgcatt ggtgctcact ttgtgtttta ctgttcatct gtgcagtatc
      721 tctgaagccc cacttacaga ggttgcaaag ggcattcccc cacagcaaac ccctcaggac
      781 cctccattgt catcagatgg aatgtacgag tatggttcta tcgagaaagt taaaaatggt
      841 tacgtaaatc cagagctggc aatgcaggga gcaaaaaaca aaaatcatgc tgaacagact
      901 cgcagggcaa tgacattaaa gtcactgctg agagcactgg tgaacatgcc tcctcactac
      961 cgctaccttt gcatcagcca cctcattgga tggacagcct tcctgtccaa catgctgttc
     1021 ttcacagatt tcatgggcca gattgtgtac cgcggggatc cctatagtgc acacaactcc
     1081 acagagtttc tcatctacga aagaggagtc gaggttggat gttggggctt gtgcatcaac
     1141 tccgtgtttt cctcacttta ttcttacttt cagaaagttt tggtatccta cattggatta
     1201 aagggtcttt acttcacggg atatttgctg tttggcctgg ggacgggatt tattgggctc
     1261 ttcccgaatg tctactccac cctggtcctg tgcagcctgt ttggtgtaat gtccagcacc
     1321 ctgtacactg tgccctttaa cctcattact gagtaccacc gcgaggaaga aaaggagagg
     1381 cagcaggccc caggagggga cccagacaac agcgtgagag ggaagggcat ggactgcgcc
     1441 accctcacat gcatggtgca gctggctcag atcctggtcg gaggtggcct gggctttctg
     1501 gtcaacacag ccgggaccgt tgtcgtcgtg gtgatcacag cgtctgcggt ggcactgata
     1561 ggctgttgct ttgtcgctct ctttgttaga tatgtggatt agtaatgaac tagtccgtaa
     1621 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga cctgaacaca
     1681 taatgtcttg ggtt
//
