LOCUS       sequence-277555-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1272
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgaaga cacctattca cacaagatgg taaaaacaaa tcataggcgc
       61 tgtcgcacaa aattcacaga agaacagttg aaaatcctca tcaatacctt caatcaaaag
      121 ccttacccag gttatgctac caaacaaaaa cttgctttag aaatcaatac agaagagtcc
      181 agaatccaga tttggtttca gaatcgaaga gctaggcacg gattccagaa aagaccagaa
      241 gctgagactt tagaatcaag ccagagccag gggcaagatc aacctggtgt ggagtttcaa
      301 agtagagaag ccagacggtg tcgtaccacc tacagcgcct ctcagttaca cactctcatc
      361 aaggcattta tgaaaaaccc atatcctggg attgattcca gagaagaact tgctaaagaa
      421 atcggtgttc cagagtcaag agtccaaatt tggttccaaa atcgaagatc tagattactt
      481 ctccagagaa aaagggaacc tgtggcgtcc ttagaacaag aagagcaggg caagattcct
      541 gagggactgc aaggtgcaga agatacacaa aatggcacca acttcactag tgactctcat
      601 ttctctggag ccagaacgtg gtagtaatga actagtccgt aacttgaaag tatttcgatt
      661 tcttggcttt atatatcttg tggaaaggac gaaagtctat tcatgatatg ccacgc
//
