LOCUS       sequence-277585-        4334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1352
FEATURES             Location/Qualifiers
     source          1..4334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggacac cgacctgttc atggaatgtg aggaggagga gttggagcca
       61 tggcagaaaa tcagtgatgt cattgaggac tctgtagttg aagattataa ttcagtggat
      121 aaaactacca cagtttctgt gagccagcag ccagtctcgg ctccagtgcc catcgctgcc
      181 catgcttctg ttgctgggca cctctctaca tccaccaccg ttagtagcag cggggcacag
      241 aacagcgaca gtacaaagaa gactcttgtc acactaattg ccaacaacaa tgctggcaat
      301 cctttggtcc agcaaggtgg acagccactc atcctgaccc agaatccagc cccaggtctg
      361 ggcacaatgg ttactcaacc agtattgagg cctgttcagg tcatgcagaa tgccaatcat
      421 gtgactagtt cccctgtggc ctcacaacca atatttatca ctacgcaggg atttcctgta
      481 aggaatgtcc ggcctgtaca aaatgcaatg aatcaggttg ggattgtgct gaacgtacag
      541 caaggccaaa cggttagacc aattacacta gttccagccc caggtaccca gtttgttaag
      601 ccgacagttg gagttccaca agtgttctcc cagatgaccc ctgtgaggcc aggctccaca
      661 atgcctgtga ggcccaccac caacaccttc accaccgtca tcccggccac tcttaccatt
      721 cgaagcaccg tcccacagtc ccagtcccag cagaccaagt ccactcccag cacttctacc
      781 actcccactg ccacacagcc aacctcactg gggcaactag ctgttcagtc tccaggccag
      841 tcaaaccaga ccacgaatcc caagctagct ccctccttcc cctctccacc tgcagtgagc
      901 attgccagct ttgtcactgt gaagcgacct ggtgttacag gcgaaaatag caatgaagtg
      961 gccaaattgg tgaataccct taacaccatc ccttccctgg gccagagtcc tgggccagtg
     1021 gtggtgtcca acaacagctc tgctcatggc tctcaaagaa ccagcggacc tgagtcttca
     1081 atgaaagtga cctcttccat cccagtattt gacctccagg atggtggacg gaaaatatgt
     1141 ccacgatgta atgctcaatt tcgtgttact gaagctttga gaggtcacat gtgttactgt
     1201 tgcccagaaa tggttgaata ccagaagaaa ggaaagtccc tggattcaga acccagtgtc
     1261 ccatcagcag caaagccccc atcccctgag aaaacagctc ctgttgcttc cacaccctct
     1321 tctacaccta ttcctgctct gtcaccgcct accaaagtac cagaaccaaa tgagaacgtg
     1381 ggcgatgccg tccagaccaa actcattatg cttgtagatg acttctacta tggacgggat
     1441 ggtggcaaag tagcccagct cacaaatttc cctaaggtcg ccacatcttt ccgatgccca
     1501 cattgtacca aaaggctaaa aaacaatatt cgattcatga accatatgaa acaccacgta
     1561 gaactcgatc agcagaacgg tgaggtagat ggtcacacta tctgccagca ctgttaccgc
     1621 cagttttcca ctcccttcca gcttcagtgc cacttggaaa atgttcatag tccctatgaa
     1681 tctactacca agtgcaagat ctgtgaatgg gcgtttgaaa gtgagccact atttctccag
     1741 catatgaagg atactcataa gcctggagag atgccttatg tttgccaggt gtgtcaatat
     1801 cgctcctcac tctactctga ggtagatgtc cattttcgga tgatccatga ggatacccgg
     1861 catctgctct gcccttattg cctgaaggtc ttcaaaaatg gcaatgcatt ccaacagcat
     1921 tacatgaggc accagaagag aaatgtttat cactgcaaca aatgccggct gcagtttctc
     1981 tttgccaagg acaaaattga acacaagctt caacaccata aaaccttccg taaacccaag
     2041 cagctggagg gcttgaaacc aggcaccaag gtgacaatcc gggcttcccg agggcagcca
     2101 cgaactgttc ctgtatcctc taatgataca cctcccagcg ccttgcagga ggcagcaccg
     2161 ctgacctcct caatggaccc tctgcctgtc ttcctttatc cccctgtcca gcgcagcatc
     2221 cagaagagag ctgttaggaa aatgagtgtc atgggccggc agacatgcct ggagtgcagc
     2281 ttcgagatcc cagacttccc taatcatttc cctacttacg tacactgctc tctgtgtcgc
     2341 tatagcacct gctgttctcg agcttatgcc aaccacatga tcaacaatca tgttccacgg
     2401 aagagcccca agtatttggc tttgtttaaa aattctgtga gtggaatcaa gctggcctgc
     2461 acttcatgta cctttgttac ctctgtgggc gatgctatgg ccaagcattt ggtattcaac
     2521 ccctctcaca gatccagcag catcctgcca cggggactca cttggatagc tcactcaagg
     2581 catggccaga ctcgtgaccg agtgcatgac cggaacgtga agaatatgta ccctcctcct
     2641 tccttcccca ctaacaaagc tgccactgtg aaatctgcgg gggccacccc agctgagcct
     2701 gaagagctac taactccctt agccccagca ctcccatcac cagcctcaac tgcaacccca
     2761 ccaccaaccc ccactcaccc gcaggcttta gcccttccac cgctggctac agagggagcc
     2821 gaatgtctga atgttgatga tcaggatgaa gggagcccag tcacccaaga acctgagcta
     2881 gcatcaggtg gtggtggtag tggtggagtt ggcaaaaagg agcagctgtc tgtgaagaag
     2941 cttcgagtag tactgtttgc tctatgctgc aatacagaac aggcagctga acacttccga
     3001 aatccccagc gacgtattcg ccgttggctt cgacgtttcc aggcctccca gggggagaat
     3061 ctagagggca aatatctgag ctttgaggca gaagagaaac tggctgagtg ggtgctaacc
     3121 cagcgcgaac aacagctacc tgtaaatgag gagaccttgt tccagaaggc caccaaaata
     3181 ggacgttctt tggaaggggg gtttaagatc tcctatgagt gggctgtgcg tttcatgctg
     3241 cggcaccacc tgactcccca tgcccggcga gctgtggccc acaccctacc taaggatgta
     3301 gcagagaatg caggactctt cattgatttt gtacaacggc agattcacaa ccaggactta
     3361 cccttgtcta tgattgtggc tattgatgag atctctttgt tcctggatac agaggtgctg
     3421 agcagtgatg atcgaaagga gaatgccctg cagacagtgg gcacagggga accttggtgt
     3481 gatgtagtcc tagccattct ggcagatggc actgtccttc ccaccctggt tttctacaga
     3541 gggcagatgg atcagcctgc taacatgcca gactccatat tgctagaggc aaaggagagt
     3601 ggctacagtg atgacgagat catggagctg tggtcaactc gagtgtggca gaagcacaca
     3661 gcttgccagc gcagcaaagg catgcttgtg atggactgtc atcgcactca cttgtcagaa
     3721 gaggtactgg ctatgcttag tgcctctagc actttgcctg cagtggtccc agcaggctgt
     3781 agctccaaaa ttcagccatt agatgtatgc atcaaaagaa ctgtcaagaa cttcctgcat
     3841 aaaaaatgga aggaacaggc tcgggaaatg gcagatactg catgtgattc tgatgtcctg
     3901 cttcagctgg tgcttgtctg gctgggtgaa gtgctaggtg tcattgggga ctgtccagag
     3961 ctagttcagc gctccttcct ggtggctagt gttctgcctg gccccgatgg caacattaac
     4021 tcacctacaa gaaatgctga catgcaggag gagctaattg cctccctaga ggagcaactg
     4081 aagctgagtg gggaacattc tgagtcttcc actccacgac ccagatcatc tcctgaagag
     4141 acaattgagc ctgaaagtct tcaccagctc tttgagggtg aaagtgagac cgagtctttc
     4201 tatggctttg aagaagctga cctagatctg atggagattt agtaatgaac tagtccgtaa
     4261 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gttgccaata
     4321 ttgatgatcg cttt
//
